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AB220039

Anti-AFF4 antibody

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(1 Publication)

Rabbit Polyclonal AFF4 antibody. Suitable for ICC and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AFF4 aa 50-200.

View Alternative Names

AF5Q31, MCEF, HSPC092, AFF4, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, Major CDK9 elongation factor-associated protein, Protein AF-5q31

1 Images
Immunocytochemistry - Anti-AFF4 antibody (AB220039)
  • ICC

Supplier Data

Immunocytochemistry - Anti-AFF4 antibody (AB220039)

Immunocytochemical analysis of formalin fixed, triton X-100 permeabilized CACO-2 cells labeling AFF4 using ab220039 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC

applications

Immunogen

Recombinant Fragment Protein within Human AFF4 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UHB7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AFF4 protein also known as AF4/FMR2 family member 4 is a transcriptional cofactor with a molecular weight of approximately 140 kDa. It plays a role in regulating transcriptional elongation by RNA polymerase II. This protein shows expression across diverse tissues with higher levels in the brain and testis. AFF4 contains domains that enable interactions with other proteins facilitating its role in transcriptional regulation.
Biological function summary

AFF4 functions as a component of the super elongation complex (SEC) which is essential for transcriptional elongation of specific genes. This complex ensures efficient transcription after the initiation phase. Through its association with SEC AFF4 influences the expression of genes involved in cellular differentiation and development. Its activity is particularly impactful on genes associated with oncogenesis and neural development affecting cellular growth and specialized cell formation.

Pathways

The function of AFF4 integrates into the transcription elongation and gene regulation pathways. AFF4 interacts with proteins like ELL2 and CDK9 which are critical components of the positive transcription elongation factor b (P-TEFb) complex. Together these proteins regulate polymerase activity and gene expression fundamental in cell cycle control and response to growth signals.

AFF4 has connections to cancer and neurological disorders. Mutations or altered expressions of AFF4 relate to acute lymphoblastic leukemia where it may form fusion proteins that disrupt normal gene expression. In neurological contexts aberrant AFF4 function can contribute to disorders such as developmental delay. Through these diseases AFF4 interacts with disease-related proteins like MLL and AF9 involved in oncogenic transformations and neurological development respectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
See full target information AFF4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:78 PubMed40000966

2025

A novel mechanism of FTO modulating the progression of endometriosis through mediating the m6A methylation of GEF-H1 in a YTHDF1-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Ding,Hua-Ying Zhang,Jia-Hao Chen,Meng-Jie Yang,Zhi-Xiong Huang,Yi-Hong Lei,Qin-Kun Sun,Jian-Bin Bai,Dian-Chao Lin,Jian-Fa Lan,Lu-Lu Ren,Zheng-Yi Chen,Wei-Dong Zhou,Qiong-Hua Chen
View all publications
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