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Goat Polyclonal AFM antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AFM aa 300-350.

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Images

Western blot - Anti-AFM antibody (AB106931), expandable thumbnail

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human AFM aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P43652

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Rat
Predicted
Cow
Predicted
Dog
Predicted

Tested
Tested

Species

Human

Dilution info

0.3-1 µg/mL

Notes

1 hour primary incubation is recommended for this product.

Predicted
Predicted

Species

Rat, Cow, Dog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed:26902720). Binds vitamin E (PubMed:12463752, PubMed:15952736). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed:15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed:19046407).

Alternative names

Recommended products

Goat Polyclonal AFM antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AFM aa 300-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human AFM aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P43652
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab106931 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

AFM also known as Afamin is a glycoprotein assigned to the albumin family. Its molecular mass is approximately 87 kDa. This protein is mainly expressed in the liver and secreted into the bloodstream. It possesses binding affinity for vitamin E which suggests a role in transport and metabolic processes. It shares structural similarities with other proteins in the albumin family contributing to its functional versatility.

Biological function summary

AFM plays a central role in the transport of lipids and vitamins particularly vitamin E throughout the body. It acts independently and does not participate in large protein complexes. AFM shows interactions with other lipid-binding proteins modulating their activities and ensuring efficient distribution of essential nutrients. Such interactions highlight the importance of AFM in maintaining cellular homeostasis and protecting cells from oxidative damage.

Pathways

AFM actively participates in metabolic and vitamin transport pathways collaborating with proteins like albumin and transthyretin. It notably influences lipid metabolism and antioxidative pathways thereby contributing to overall cellular defense mechanisms. AFM's functionality intertwines with the activities of these proteins indicating its essential role in finely tuned biological processes especially those involving lipid and vitamin management.

Associated diseases and disorders

AFM has associations with metabolic and neurodegenerative conditions. Altered levels of AFM can correlate with diseases like type 2 diabetes and Alzheimer's disease. It interacts with other relevant proteins such as apolipoproteins impacting disease progression and symptomatology. These connections suggest that AFM could potentially be a biomarker or therapeutic target for managing such disorders opening avenues for future research and treatment strategies.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Western blot - Anti-AFM antibody (ab106931), expandable thumbnail

    Western blot - Anti-AFM antibody (ab106931)

    All lanes: Western blot - Anti-AFM antibody (ab106931) at 0.3 µg/mL

    All lanes: Human Cerebellum lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 69 kDa

    Observed band size: 80 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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