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Rabbit Polyclonal AFM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AFM aa 350 to C-terminus.

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Images

Western blot - Anti-AFM antibody (AB152141), expandable thumbnail
  • Western blot - Anti-AFM antibody (AB152141), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AFM antibody (AB152141), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFM antibody (AB152141), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human AFM aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P43652

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Species

Human

Dilution info

1/1000.00000 - 1/10000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B (PubMed:26902720). Binds vitamin E (PubMed:12463752, PubMed:15952736). May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient (PubMed:15952736). May be involved in the transport of vitamin E across the blood-brain barrier (PubMed:19046407).

Alternative names

Recommended products

Rabbit Polyclonal AFM antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human AFM aa 350 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human AFM aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P43652
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

AFM also known as Afamin is a glycoprotein assigned to the albumin family. Its molecular mass is approximately 87 kDa. This protein is mainly expressed in the liver and secreted into the bloodstream. It possesses binding affinity for vitamin E which suggests a role in transport and metabolic processes. It shares structural similarities with other proteins in the albumin family contributing to its functional versatility.

Biological function summary

AFM plays a central role in the transport of lipids and vitamins particularly vitamin E throughout the body. It acts independently and does not participate in large protein complexes. AFM shows interactions with other lipid-binding proteins modulating their activities and ensuring efficient distribution of essential nutrients. Such interactions highlight the importance of AFM in maintaining cellular homeostasis and protecting cells from oxidative damage.

Pathways

AFM actively participates in metabolic and vitamin transport pathways collaborating with proteins like albumin and transthyretin. It notably influences lipid metabolism and antioxidative pathways thereby contributing to overall cellular defense mechanisms. AFM's functionality intertwines with the activities of these proteins indicating its essential role in finely tuned biological processes especially those involving lipid and vitamin management.

Associated diseases and disorders

AFM has associations with metabolic and neurodegenerative conditions. Altered levels of AFM can correlate with diseases like type 2 diabetes and Alzheimer's disease. It interacts with other relevant proteins such as apolipoproteins impacting disease progression and symptomatology. These connections suggest that AFM could potentially be a biomarker or therapeutic target for managing such disorders opening avenues for future research and treatment strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-AFM antibody (ab152141), expandable thumbnail

    Western blot - Anti-AFM antibody (ab152141)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-AFM antibody (ab152141) at 1/5000 dilution

    Lane 1: 293T whole cell lysate at 30 µg

    Lane 2: A431 whole cell lysate at 30 µg

    Lane 3: HeLa whole cell lysate at 30 µg

    Lane 4: HepG2 whole cell lysate at 30 µg

    Predicted band size: 69 kDa

  • Western blot - Anti-AFM antibody (ab152141), expandable thumbnail

    Western blot - Anti-AFM antibody (ab152141)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-AFM antibody (ab152141) at 1/5000 dilution

    Lane 1: NIH 3T3 whole cell lysate at 30 µg

    Lane 2: JC whole cell lysate at 30 µg

    Lane 3: BCL-1 whole cell lysate at 30 µg

    Predicted band size: 69 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AFM antibody (ab152141), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AFM antibody (ab152141)

    Immunofluorescent analysis of methanol-fixed HeLa cells labeling AFM with ab152141 at 1/500 dilution. Lower image co-stained with Hoerchst 33342.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFM antibody (ab152141), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFM antibody (ab152141)

    Immunohistochemical analysis of paraffin-embedded Cal27 (human) xenograft labeling AFM with ab152141 at 1/500 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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