Anti-AFM antibody [EPR19383] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-AFM antibody [EPR19383] - BSA and Azide free (AB251332)

This data was developed using ab201034, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-5 and 7 : 30 seconds; Lane 6 : 3 seconds.

All lanes:

Western blot - Anti-AFM antibody [EPR19383] (<a href='/en-us/products/primary-antibodies/afm-antibody-epr19383-ab201034'>ab201034</a>) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human blood at 20 µg

Lane 3:

Mouse plasma at 20 µg

Lane 4:

Mouse serum at 20 µg

Lane 5:

Rat serum at 20 µg

Lane 6:

Rat plasma at 20 µg

Lane 7:

Human plasma at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 87 kDa

false

• WB

Supplier Data

Western blot - Anti-AFM antibody [EPR19383] - BSA and Azide free (AB251332)

This data was developed using ab201034, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

AFM is a glycoprotein, its apparent molecular weight on SDS-PAGE is 87 kDa due to glycosylation. AFM has 4 potential sites for N-Glycosylation and N-glycanase treatment reduced the apparent MW to 65 kDa (PMID : 7517938).

All lanes:

Western blot - Anti-AFM antibody [EPR19383] (<a href='/en-us/products/primary-antibodies/afm-antibody-epr19383-ab201034'>ab201034</a>) at 1/1000 dilution

Lane 1:

Untreated mouse serum at 10 µg

Lane 2:

Mouse serum PNGase F treated at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 65 kDa,87 kDa

false

Exposure time: 5s