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AB122239

Anti-Aftiphilin antibody

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(1 Publication)

Rabbit Polyclonal Aftiphilin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AFTPH aa 250-350.

View Alternative Names

AFTH, AFTPH, Aftiphilin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aftiphilin antibody (AB122239)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aftiphilin antibody (AB122239)

ab122239, at 1/500 dilution, staining Aftiphilin in paraffin-embedded Human smooth muscle by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-Aftiphilin antibody (AB122239)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aftiphilin antibody (AB122239)

ab122239, at 4μg/ml, staining Aftiphilin in Human cell line U-2 OS by Immunofluorescence. Antibody staining is shown in green.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AFTPH aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q6ULP2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab122239 is mono-specific.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aftiphilin is a protein involved in the formation of vesicles and transport processes in the cell. Known alternatively as AFTPH it weighs around 80 kDa. Researchers identify its expression mainly in various tissues especially in the brain and some epithelial cells. Its primary role is associated with its function in clathrin-mediated endocytosis a process critical for the internalization of molecules at the cellular membrane.
Biological function summary

Aftiphilin operates by interacting with other proteins to facilitate vesicle trafficking. It is a component of a larger complex that includes p200 and epsinR where it plays a role in stabilizing clathrin-coated vesicles. These interactions suggest its importance in maintaining cellular transport dynamics and in various membrane-related processes. Its precise functioning influences several biological systems by ensuring that cellular materials are properly transported and sorted.

Pathways

Aftiphilin participates in the clathrin-mediated endocytosis pathway and the Golgi-endosomal transport pathway. Within these pathways aftiphilin interacts with clathrin and AP-1 both of which assist in ensuring that molecular cargo is accurately directed and delivered within the cell. These pathways are essential for maintaining cellular homeostasis and are key to the efficient recycling and redistribution of membrane components.

Altered function or expression of aftiphilin links to certain neurological conditions and cancer. For instance dysregulation in aftiphilin activity associates with Alzheimer’s disease where it may affect protein trafficking and clearance leading to toxic accumulation. Similarly aftiphilin can interact with cancer-related proteins like EGFR potentially affecting tumor progression through misregulated signaling and endocytosis processes. Understanding these connections highlights aftiphilin's role in both health and disease offering potential paths for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of clathrin-coated vesicles (PubMed : 15758025). Component of the aftiphilin/p200/gamma-synergin complex, which plays roles in AP1G1/AP-1-mediated protein trafficking including the trafficking of transferrin from early to recycling endosomes, and the membrane trafficking of furin and the lysosomal enzyme cathepsin D between the trans-Golgi network (TGN) and endosomes (PubMed : 15758025).
See full target information AFTPH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 45: PubMed33786634

2021

acts via the miR‑922/ axis to enhance malignant behavior of liver cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Liu,Hao Zhang,Pengcheng Zhou,Yaqun Yu,Haoye Zhang,Limin Chen,Jian Gong,Zhenguo Liu
View all publications
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Product promise

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