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AB201464

Anti-AG-3 antibody [EPR19606]

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(1 Publication)

Rabbit Recombinant Monoclonal AG-3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BCMP11, PDIA18, UNQ642/PRO1272, AGR3, Anterior gradient protein 3, AG-3, AG3, hAG-3, Anterior gradient 3 homolog, Breast cancer membrane protein 11

3 Images
Flow Cytometry (Intracellular) - Anti-AG-3 antibody [EPR19606] (AB201464)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AG-3 antibody [EPR19606] (AB201464)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] (AB201464)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] (AB201464)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab201464 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-AG-3 antibody [EPR19606] (AB201464)
  • WB

Supplier Data

Western blot - Anti-AG-3 antibody [EPR19606] (AB201464)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1/5 : 15 seconds; Lane 2-4 : 3 minutes.

Lanes 1 and 5:

Western blot - Anti-AG-3 antibody [EPR19606] (ab201464) at 1/2000 dilution

Lane 2:

Western blot - Anti-AG-3 antibody [EPR19606] (ab201464) at 1/10000 dilution

Lanes 3 - 4:

Western blot - Anti-AG-3 antibody [EPR19606] (ab201464) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 2:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

Lane 3:

Human colon cancer tissue lysate at 10 µg

Lane 4:

Human breast cancer tissue lysate at 10 µg

Lane 5:

Human ovary cancer tissue lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 5:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

  • Carrier free

    Anti-AG-3 antibody [EPR19606] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19606

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AG-3 also known as protein AG is a protein of about 45 kDa. This target is predominantly expressed in the cytoplasm of liver cells though it can also be found in other tissues in lower amounts. AG-3 has been identified as a protein that plays an important role in various cellular processes. Its structural domains allow it to interact with a range of biomolecules which can contribute to its diverse functions in the cell.
Biological function summary

AG-3 enables essential cellular processes such as signal transduction and metabolic regulation. As a component of a larger protein complex AG-3 helps maintain cellular homeostasis by modulating enzymatic activities. Its presence in the complex allows for precise control and fine-tuning of cellular responses to external stimuli. Moreover AG-3 facilitates the transportation of important metabolites and ions ensuring proper cellular functionality.

Pathways

AG-3 significantly contributes to the MAPK signaling pathway and the insulin signaling pathway. Within these pathways AG-3 interacts with proteins like ERK1/2 and IRS proteins playing a role in cellular proliferation differentiation and metabolism. The involvement of AG-3 in these pathways exemplifies its importance in controlling cellular growth and metabolic processes making it a focal point for studies related to these functions.

AG-3 is linked to conditions like type 2 diabetes and liver diseases. Alterations in AG-3 expression or function can disrupt normal cellular processes leading to the development or progression of these diseases. AG-3 interacts with insulin signaling proteins such as IRS-1 and any imbalance can contribute to insulin resistance and metabolic syndrome. Similarly aberrant levels of AG-3 in liver cells are associated with liver dysfunction suggesting a critical role for AG-3 in maintaining liver health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.
See full target information AGR3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research and treatment : official journal o 52:218-245 PubMed31291711

2019

Anterior Gradient 3 Promotes Breast Cancer Development and Chemotherapy Response.

Applications

Unspecified application

Species

Unspecified reactive species

Qiao Xu,Ying Shao,Jinman Zhang,Huikun Zhang,Yawen Zhao,Xiaoli Liu,Zhifang Guo,Wei Chong,Feng Gu,Yongjie Ma
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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