Goat Polyclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Agarose. Suitable for IP and reacts with Tag samples. Cited in 6 publications.
pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0268% PBS
| IP | |
|---|---|
| Tag | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
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DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
Goat Polyclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Agarose. Suitable for IP and reacts with Tag samples. Cited in 6 publications.
pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0268% PBS
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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