AB1240

Agarose Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Name: Anti-DDDDK tag (Binds to FLAG® tag sequence) Agarose antibody (ab1240) | Abcam
Brand: Abcam Limited
SKU: AB1240
Price: 535 USD
Availability: InStock

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(9 Publications)

Goat Polyclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Agarose. Suitable for IP and reacts with Tag samples. Cited in 9 publications.

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Agarose

Excitation/Emission

Carrier free

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }

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Product details

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.58% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1109 PubMed39875384

2025

Activation of lysophagy by a TBK1-SCF-TMEM192-TAX1BP1 axis in response to lysosomal damage.

Applications

Unspecified application

Species

Unspecified reactive species

Na Yeon Park,Doo Sin Jo,Jae-Yoon Yang,Ji-Eun Bae,Joon Bum Kim,Yong Hwan Kim,Seong Hyun Kim,Pansoo Kim,Dong-Seok Lee,Tamotsu Yoshimori,Eun-Kyeong Jo,Eunbyul Yeom,Dong-Hyung Cho

Autophagy 21:664-673 PubMed39477686

2024

Deciphering melanophagy: role of the PTK2-ITCH-MLANA-OPTN cascade on melanophagy in melanocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Na Yeon Park,Doo Sin Jo,Hyun Jun Park,Ji-Eun Bae,Yong Hwan Kim,Joon Bum Kim,Ha Jung Lee,Sung Hyun Kim,Hyunjung Choi,Hyun-Shik Lee,Tamotsu Yoshimori,Dong-Seok Lee,Jin-A Lee,Pansoo Kim,Dong-Hyung Cho

Plant biotechnology journal 21:943-960 PubMed36632734

2023

The C H -type zinc finger transcription factor OSIC1 positively regulates stomatal closure under osmotic stress in poplar.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuxian Bai,Zhimin Niu,Qingyuan Chen,Chengyu Gao,Mingjia Zhu,Jiexian Bai,Meijun Liu,Ling He,Jianquan Liu,Yuanzhong Jiang,Dongshi Wan

The New phytologist : PubMed33492673

2021

CHYR1 ubiquitinates the phosphorylated WRKY70 for degradation to balance immunity in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Liu,Bao Liu,Shangling Lou,Hao Bi,Hu Tang,Shaofei Tong,Song Yan,Ningning Chen,Han Zhang,Yuanzhong Jiang,Jianquan Liu

The New phytologist 229:106-125 PubMed33098101

2020

WRKY33 interacts with WRKY12 protein to up-regulate RAP2.2 during submergence induced hypoxia response in Arabidopsis thaliana.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Tang,Hao Bi,Bao Liu,Shangling Lou,Yan Song,Shaofei Tong,Ningning Chen,Yuanzhong Jiang,Jianquan Liu,Huanhuan Liu

Scientific reports 9:12437 PubMed31455821

2019

PIDD interaction with KEAP1 as a new mutation-independent mechanism to promote NRF2 stabilization and chemoresistance in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Ji,Rui Zhang,Jie Chen,Qun Xue,Nadeem Moghal,Ming-Sound Tsao

PLoS pathogens 11:e1004771 PubMed25794275

2015

NEDDylation is essential for Kaposi's sarcoma-associated herpesvirus latency and lytic reactivation and represents a novel anti-KSHV target.

Applications

Species

Human

David J Hughes,Jennifer J Wood,Brian R Jackson,Belinda Baquero-Pérez,Adrian Whitehouse

The Journal of cell biology 207:717-33 PubMed25533843

2014

Opposing ISWI- and CHD-class chromatin remodeling activities orchestrate heterochromatic DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Karolin Klement,Martijn S Luijsterburg,Jordan B Pinder,Chad S Cena,Victor Del Nero,Christopher M Wintersinger,Graham Dellaire,Haico van Attikum,Aaron A Goodarzi

Oncogene 31:5038-44 PubMed22286768

2012

The ShcA SH2 domain engages a 14-3-3/PI3'K signaling complex and promotes breast cancer cell survival.

Applications

Species

Unspecified reactive species

J Ursini-Siegel,W R Hardy,Y Zheng,C Ling,D Zuo,C Zhang,L Podmore,T Pawson,W J Muller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Agarose Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (9)

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