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Goat Polyclonal V5 tag antibody - conjugated to Agarose. Suitable for IP and reacts with Tag samples. Cited in 18 publications.


Key facts

Isotype

IgG

Host species

Goat

Conjugation

Agarose

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.58% Sodium chloride, 0.0268% PBS

Form

Liquid

Clonality

Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IP
Tag
Expected

Expected
Expected

Species

Tag

Dilution info

-

Notes

Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.

Alternative names

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Goat Polyclonal V5 tag antibody - conjugated to Agarose. Suitable for IP and reacts with Tag samples. Cited in 18 publications.

Alternative names

Key facts

Isotype

IgG

Conjugation

Agarose

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Activity summary

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Associated diseases and disorders

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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