AB1229

Agarose Anti-V5 tag antibody

Name: Agarose Anti-V5 tag antibody (ab1229) | Abcam
Brand: Abcam Limited
SKU: AB1229
Rating: 5 (1 reviews)

(1 Review)

(23 Publications)

Goat Polyclonal V5 tag antibody - conjugated to Agarose. Suitable for IP and reacts with Tag samples. Cited in 23 publications.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Agarose

Excitation/Emission

Carrier free

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use at a concentration of 15 - 20 µl of gel slurry per 0.1 to 1 mg of protein lysate or extract.</p>" } } }

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Product details

Provided as 400µl of slurry (200µl gel + 200µl buffer). 100µg of the affinity purified antibody is attached to the gel at a ratio of 500µg antibody/ml of gel or 250µg IgG/ml of 50% slurry.Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.58% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The V5 tag is an epitope tag derived from the P and V fusion proteins of the paramyxovirus. It typically measures around 14 amino acids in length and is often used in molecular biology for tagging and protein expression studies. In terms of mechanical function the V5 tag facilitates the detection characterization and purification of recombinant proteins. Scientists commonly use it in various hosts such as bacteria yeast and mammalian cells due to its convenient integration into larger protein complexes. Unlike native proteins it serves as a non-disruptive label for identifying proteins without affecting their functionality.

Biological function summary

Researchers employ the V5 tag to decipher protein localization expression and role within cellular environments. It allows easy tracking in situ by using antibodies that target the V5 epitope such as the anti-V5 antibody. The V5 tag proves effective in dual-tagging systems useful in elucidating protein interactions and complex formations for instance when combined with other tags like biotin tag or APC tag. This assists in isolating and identifying components of complex cellular machinery.

Pathways

The tag is frequently utilized in studies exploring functional pathways of various proteins across metabolic and signaling pathways. It aids in examining the relationship between proteins within signaling cascades by tagging proteins that interact with others such as those in the PK tag-related signaling pathways. Researchers often track proteins across intricate networks to understand cellular processes intricately using V5-tagged proteins as indicators or reporters.

Scientists harness the V5 tag to investigate the expression and interactions of proteins associated with certain conditions such as cancer and neurodegenerative diseases. Through its integration scientists aim to uncover alterations in protein interactions or localizations that contribute to disease progression. For example they may tag proteins linked to cancer pathways observing how disruptions in proteins related to anti-V5 targets could provide insights into tumor biology or pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information V5 tag

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38950322

2024

CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Nunziata Maio,Rotem Orbach,Irina T Zaharieva,Ana Töpf,Sandra Donkervoort,Pinki Munot,Juliane Mueller,Tracey Willis,Sumit Verma,Stojan Peric,Deepa Krishnakumar,Sniya Sudhakar,A Reghan Foley,Sarah Silverstein,Ganka Douglas,Lynn Pais,Stephanie DiTroia,Christopher Grunseich,Ying Hu,Caroline Sewry,Anna Sarkozy,Volker Straub,Francesco Muntoni,Tracey A Rouault,Carsten G Bönnemann

Nature communications 15:371 PubMed38191531

2024

Deletion of Aurora kinase A prevents the development of polycystic kidney disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Shen Tham,Denny L Cottle,Allara K Zylberberg,Kieran M Short,Lynelle K Jones,Perkin Chan,Sarah E Conduit,Jennifer M Dyson,Christina A Mitchell,Ian M Smyth

Cell reports 42:112455 PubMed37149867

2023

A tug of war between DCC and ROBO1 signaling during commissural axon guidance.

Applications

Unspecified application

Species

Unspecified reactive species

Brianna Dailey-Krempel,Andrew L Martin,Ha-Neul Jo,Harald J Junge,Zhe Chen

Nature communications 13:4188 PubMed35858913

2022

Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Noemi Gioelli,Lisa J Neilson,Na Wei,Giulia Villari,Wenqian Chen,Bernhard Kuhle,Manuel Ehling,Federica Maione,Sander Willox,Serena Brundu,Daniele Avanzato,Grigorios Koulouras,Massimiliano Mazzone,Enrico Giraudo,Xiang-Lei Yang,Donatella Valdembri,Sara Zanivan,Guido Serini

Journal of genetics and genomics = Yi chuan xue ba 47:187-199 PubMed32499180

2020

Drosophila P75 safeguards oogenesis by preventing H3K9me2 spreading.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Dou,Yanchao Liu,Yingpei Zhang,Chenhui Wang,Ying Huang,Zz Zhao Zhang

Nature biotechnology 37:1493-1501 PubMed31548729

2019

Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adrian Pickar-Oliver,Joshua B Black,Mae M Lewis,Kevin J Mutchnick,Tyler S Klann,Kylie A Gilcrest,Madeleine J Sitton,Christopher E Nelson,Alejandro Barrera,Luke C Bartelt,Timothy E Reddy,Chase L Beisel,Rodolphe Barrangou,Charles A Gersbach

Cell 178:1421-1436.e24 PubMed31491386

2019

Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel S Greenberg,Hannah K Long,Tomek Swigut,Joanna Wysocka

RNA biology 16:1682-1696 PubMed31432740

2019

Identification of the Selenoprotein S Positive UGA Recoding (SPUR) element and its position-dependent activity.

Applications

Unspecified application

Species

Unspecified reactive species

Eric M Cockman,Vivek Narayan,Belinda Willard,Sumangala P Shetty,Paul R Copeland,Donna M Driscoll

Neuroscience 404:282-296 PubMed30742966

2019

A Species-Correlated Transitional Residue D132 on Human FMRP Plays a Role in Nuclear Localization via an RNA-Dependent Interaction With PABP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Ting Zhou,Jing-Yi Long,Jun-Yi Fu,Wei-Wen Sun,Fei Hu,Hao-Ying Huang,Wei Li,Mei-Mei Gao,Yousheng Shu,Yong-Hong Yi,Yue-Sheng Long

The EMBO journal 37: PubMed29925518

2018

Cilia-localized LKB1 regulates chemokine signaling, macrophage recruitment, and tissue homeostasis in the kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Viau,Frank Bienaimé,Kamile Lukas,Abhijeet P Todkar,Manuel Knoll,Toma A Yakulov,Alexis Hofherr,Oliver Kretz,Martin Helmstädter,Wilfried Reichardt,Simone Braeg,Tom Aschman,Annette Merkle,Dietmar Pfeifer,Verónica I Dumit,Marie-Claire Gubler,Roland Nitschke,Tobias B Huber,Fabiola Terzi,Jörn Dengjel,Florian Grahammer,Michael Köttgen,Hauke Busch,Melanie Boerries,Gerd Walz,Antigoni Triantafyllopoulou,E Wolfgang Kuehn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Agarose Anti-V5 tag antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise