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AB235778

Anti-AGBL2 antibody

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(1 Publication)

Rabbit Polyclonal AGBL2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGBL2 aa 1-300.

View Alternative Names

CCP2, AGBL2, Cytosolic carboxypeptidase 2, ATP/GTP-binding protein-like 2, Protein deglutamylase CCP2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGBL2 antibody (AB235778)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGBL2 antibody (AB235778)

Paraffin-embedded human breast cancer tissue stained for AGBL2 using ab235778 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGBL2 antibody (AB235778)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGBL2 antibody (AB235778)

Paraffin-embedded human colon cancer tissue stained for AGBL2 using ab235778 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-AGBL2 antibody (AB235778)
  • WB

Supplier Data

Western blot - Anti-AGBL2 antibody (AB235778)

All lanes:

Western blot - Anti-AGBL2 antibody (ab235778) at 1/500 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AGBL2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q5U5Z8

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB191866

VHH Single Domain Anti-Rabbit IgG Fc (HRP)

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (AB191866)

  • 5
  • 2
Secondary Antibodies

AB150171

Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (AB150171)

  • 5
  • 2
Proteins & Peptides

AB127139

Recombinant Human AGBL2 protein

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as Metalloprotease Three and ATP/GTP Binding Protein-Like 2 or AGBL2 is part of the cytoplasmic carboxypeptidase family. This enzyme functions by removing glutamate residues from tubulin modifying microtubule properties and dynamics. AGBL2 has a molecular mass of around 83 kilodaltons. Researchers have identified its expression in several tissues including the brain liver and heart suggesting a broad role in the human body.
Biological function summary

AGBL2 participates in the regulation of neuronal microtubule stability. It forms a part of the cytosolic complex that influences cellular functions including maintaining the integrity of the cytoskeleton. AGBL2's activity is essential for the post-translational modification of tubulin which affects cell cycle progression and neuronal signaling. By modulating microtubule behavior AGBL2 contributes importantly to processes like intracellular transport and cell differentiation.

Pathways

AGBL2 actively engages in the regulation of the cytoskeleton. It is involved in the tubulin detyrosination/tyrosination cycle and interacts with other proteins like tubulin and MAP (microtubule-associated proteins) to stabilize or destabilize microtubules as needed. AGBL2 also plays roles in influencing the Wnt signaling pathway critical for developmental processes and cellular communication. By doing so it links with other regulatory proteins to modulate cellular responses.

Researchers have connected AGBL2 to neurodegenerative diseases and cancer. Abnormal expression or activity of AGBL2 can lead to disrupted microtubule dynamics causing neurological conditions such as Alzheimer's disease. AGBL2 interacts with proteins like tau whose hyperphosphorylation is a hallmark of Alzheimer's. Moreover in some cancers altered AGBL2 activity may affect cell proliferation by impacting microtubule stability indicating potential therapeutic targets for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein. Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate. Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK.
See full target information AGBL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:405 PubMed40393967

2025

Cellular crosstalk mediated by Meteorin-like regulating hepatic stellate cell activation during hepatic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxia Zhou,Guifang Wang,Lingling Liu,Lingyu Song,Laying Hu,Lu Liu,Lifen Xu,Tuanlao Wang,Lirong Liu,Yuanyuan Wang,Tian Zhang,Bing Guo
View all publications
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Product promise

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