Anti-AGE (Advanced Glycation End-products) antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-AGE (Advanced Glycation End-products) antibody (AB23722)

ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

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Western blot - Anti-AGE (Advanced Glycation End-products) antibody (AB23722)

AGE western blot using Anti-AGE antibody ab23722. Publication image and figure legend from Sakellariou, S., Fragkou, P., et al., 2016, BMC Cancer, PubMed 26931562.

Western immunoblot indicating AGEs, RAGE and GLO-I protein expression in two CRC and adjacent normal tissues a. The densitometric quantification of AGEs and GLO-I expression (normalized to the actin levels) is shown in the graph b. Western immunoblot indicating AGEs, RAGE and GLO-I protein expression in tree colon cancer cell lines SW480, HT29 and HCT116 c All experiments have been performed at least three times and representative results of one experiment are shown

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• IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-AGE (Advanced Glycation End-products) antibody (AB23722)

AGE immunohistochemistry-immunofluorescence using Anti-AGE antibody ab23722. Publication image and figure legend from Byun, K., Bayarsaikhan, D., et al., 2014, PLoS One, PubMed 25140518.

Distribution of AGE-albumin and activated microglial cells in the brains of human alcoholics and control people.(A) Triple-labeled confocal microscopic image analyses were used to study the distribution and relative levels of AGE (blue), albumin (green), and Iba-1 (red) in the cerebral cortex of human brains from normal people or alcoholic individuals. (B) Densitometry analyses of AGE, albumin, and Iba-1 colocalization were evaluated using Zeiss Zen software. Scale bar = 50 µm. **p<0.001.