Anti-AGE antibody ab23722 is a rabbit polyclonal antibody that is used in AGE western blotting, IHC and immunofluorescence.
- Tried and trusted by researchers since 2005
IgG
Rabbit
pH: 7.15
Preservative: 0.05% Sodium azide
Constituents: 0.85% Sodium chloride, 0.134% PBS
Liquid
Polyclonal
IHC-P | IHC-Fr | WB | ELISA | ICC/IF | |
---|---|---|---|---|---|
Chemical | Expected | Expected | Expected | Not recommended | Tested |
Purified native protein | Not recommended | Not recommended | Not recommended | Expected | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Purified native protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Purified native protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Purified native protein | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Purified native protein | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chemical | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Purified native protein | Dilution info - | Notes - |
Select an associated product type
AGEs, Advanced glycation end products
Anti-AGE antibody ab23722 is a rabbit polyclonal antibody that is used in AGE western blotting, IHC and immunofluorescence.
- Tried and trusted by researchers since 2005
IgG
Rabbit
pH: 7.15
Preservative: 0.05% Sodium azide
Constituents: 0.85% Sodium chloride, 0.134% PBS
Liquid
Polyclonal
Affinity purification Protein A
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
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This supplementary information is collated from multiple sources and compiled automatically.
Advanced Glycation End-products (AGEs) are highly reactive compounds formed when reducing sugars non-enzymatically bind to proteins lipids or nucleic acids. They are often referred to as AGE products or AGE-BSA when in the form of bovine serum albumin (BSA). The molecular weight of AGE compounds can vary significantly due to the diversity of glycation products. AGEs express in various tissues but accumulate more in aging tissues and in certain pathological states. Detection of AGE products often utilizes AGE ELISA assays or fluorescence-based methods to measure AGE-BSA.
AGE compounds affect cellular and tissue functions by altering structural proteins and increasing oxidative stress. While not a component of a specific complex AGEs catalyze cross-linking of proteins like collagen impacting tissue elasticity. The binding of AGEs to receptors such as RAGE (Receptor for Advanced Glycation End-products) triggers inflammatory pathways and oxidative stress. This interaction fuels the damaging effects of AGEs in biological systems which are assayed through AGE assays.
AGE compounds influence several critical biological pathways particularly the NF-kB and MAPK pathways. These pathways mediate inflammation and cellular stress responses. The interaction with RAGE also affects signaling with related proteins like S100/calgranulins which further activate pro-inflammatory responses. The alteration of these pathways by AGE compounds highlights their significance in cellular dysfunction and age-related conditions.
AGE compounds play significant roles in the progression of diabetes and cardiovascular disease. In diabetes AGEs contribute to complications by modifying vascular structure and function where they link with proteins such as HbA1c in glycation. In cardiovascular disease AGE accumulation in vascular tissues enhances vascular stiffness and atherosclerotic lesion formation. The AGE-RAGE interaction perpetuates chronic inflammation further exacerbating these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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