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Anti-AGE antibody ab23722 is a rabbit polyclonal antibody that is used in AGE western blotting, IHC and immunofluorescence.

- Tried and trusted by researchers since 2005


Images

Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (AB23722), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.15
Preservative: 0.05% Sodium azide
Constituents: 0.85% Sodium chloride, 0.134% PBS

Form

Liquid

Clonality

Polyclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIHC-FrWBELISAICC/IF
Chemical
Expected
Expected
Expected
Not recommended
Tested
Purified native protein
Not recommended
Not recommended
Not recommended
Expected
Not recommended

Expected
Expected

Species

Chemical

Dilution info

1/10000

Notes

-

Not recommended
Not recommended

Species

Purified native protein

Dilution info

-

Notes

-

Expected
Expected

Species

Chemical

Dilution info

1/2000

Notes

-

Not recommended
Not recommended

Species

Purified native protein

Dilution info

-

Notes

-

Expected
Expected

Species

Chemical

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Purified native protein

Dilution info

-

Notes

-

Expected
Expected

Species

Purified native protein

Dilution info

1 µg/mL

Notes

-

Not recommended
Not recommended

Species

Chemical

Dilution info

-

Notes

-

Tested
Tested

Species

Chemical

Dilution info

5 µg/mL

Notes

-

Not recommended
Not recommended

Species

Purified native protein

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Alternative names

Recommended products

Anti-AGE antibody ab23722 is a rabbit polyclonal antibody that is used in AGE western blotting, IHC and immunofluorescence.

- Tried and trusted by researchers since 2005

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Advanced Glycation End-products (AGEs) are highly reactive compounds formed when reducing sugars non-enzymatically bind to proteins lipids or nucleic acids. They are often referred to as AGE products or AGE-BSA when in the form of bovine serum albumin (BSA). The molecular weight of AGE compounds can vary significantly due to the diversity of glycation products. AGEs express in various tissues but accumulate more in aging tissues and in certain pathological states. Detection of AGE products often utilizes AGE ELISA assays or fluorescence-based methods to measure AGE-BSA.

Biological function summary

AGE compounds affect cellular and tissue functions by altering structural proteins and increasing oxidative stress. While not a component of a specific complex AGEs catalyze cross-linking of proteins like collagen impacting tissue elasticity. The binding of AGEs to receptors such as RAGE (Receptor for Advanced Glycation End-products) triggers inflammatory pathways and oxidative stress. This interaction fuels the damaging effects of AGEs in biological systems which are assayed through AGE assays.

Pathways

AGE compounds influence several critical biological pathways particularly the NF-kB and MAPK pathways. These pathways mediate inflammation and cellular stress responses. The interaction with RAGE also affects signaling with related proteins like S100/calgranulins which further activate pro-inflammatory responses. The alteration of these pathways by AGE compounds highlights their significance in cellular dysfunction and age-related conditions.

Associated diseases and disorders

AGE compounds play significant roles in the progression of diabetes and cardiovascular disease. In diabetes AGEs contribute to complications by modifying vascular structure and function where they link with proteins such as HbA1c in glycation. In cardiovascular disease AGE accumulation in vascular tissues enhances vascular stiffness and atherosclerotic lesion formation. The AGE-RAGE interaction perpetuates chronic inflammation further exacerbating these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)

    ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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