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Mouse Recombinant Monoclonal Aggrecan antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 199 publications.


Images

Western blot - Anti-Aggrecan antibody [6-B-4] (AB3778), expandable thumbnail
  • Western blot - Anti-Aggrecan antibody [6-B-4] (AB3778), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (AB3778), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (AB3778), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
0.05 µg/mL
Notes

Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Alternative names

AGC1, CSPG1, MSK16, ACAN, Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, Chondroitin sulfate proteoglycan 1

Recommended products

Mouse Recombinant Monoclonal Aggrecan antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 199 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
6-B-4
Purification technique
Affinity purification Protein A
Specificity

This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with this antibody. However, there are many citations indicating this antibody is able to detect Aggrecan in endogenous sample lysates.

Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 08th October 2020.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aggrecan also known as aggrecan core protein or chondroitin sulfate proteoglycan 1 is a large proteoglycan vital for cartilage structure and function. It has a mass of approximately 250 kDa. Aggrecan is expressed mostly in cartilage regions such as articular cartilage but it might also be found in other connective tissues. The structure of aggrecan consists of a core protein that attaches to glycosaminoglycan chains allowing it to create hydrated gel structures essential for the resilience and load-bearing properties of cartilage.

Biological function summary

Aggrecan plays a significant role in maintaining the structural integrity of cartilage by providing resistance to compression. This proteoglycan is a central component of the extracellular matrix forming large aggregates with hyaluronic acid and link proteins. These aggregates contribute to the cartilage's osmotic properties helping resist compression during joint movement. Aggrecan's structural properties make it necessary for normal joint function and health.

Pathways

Aggrecan interacts within the cartilage development and maintenance frameworks. Specifically it participates in the regulation of the ECM-receptor interaction pathway and the proteoglycans in cancer pathway. Aggrecan functions alongside proteins such as type II collagen to ensure biomechanical properties of the cartilage. Additionally its enzymatic breakdown by aggrecanases like ADAMTS can impact these pathways leading to various physiological effects.

Associated diseases and disorders

Aggrecan is closely associated with osteoarthritis and intervertebral disc degeneration. In osteoarthritis the loss of aggrecan through enzymatic degradation leads to reduced joint function and increased pain. The breakdown involves aggrecanases and other matrix metalloproteinases resulting in cartilage degradation. Diseases such as intervertebral disc degeneration also link to the reduced presence or altered structure of aggrecan impacting spine health and mobility.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778), expandable thumbnail

    Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

    Buffer and Concentration: 2% BSA/TBST

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778) at 1/1000 dilution

    All lanes: C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675) 15ng

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 250 kDa

  • Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778), expandable thumbnail

    Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

    ab3778 was shown to react with recombinant human Aggrecan protein in western blot.

    Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1μg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at a 1/20000 dilution for 1 hour at RT.

    All lanes: Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

    Predicted band size: 250 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

    Secreted staining of cartilage cells.

    Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (ab3778)

    No staining in negative control tissue (human normal liver).

    Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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