Anti-Aggrecan antibody [6-B-4]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (AB3778)

Secreted staining of cartilage cells.

Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [6-B-4] (AB3778)

No staining in negative control tissue (human normal liver).

Staining performed on a Leica BOND RXTM system using the standard protocol F. The section was pre-treated using enzymatic retrieval, for 15mins. The section was then incubated with ab3778, 0.05μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

Supplier Data

Western blot - Anti-Aggrecan antibody [6-B-4] (AB3778)

ab3778 was shown to react with recombinant human Aggrecan protein in western blot.

Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab3778 at 1μg/ml overnight at 4°C. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye®800CW) preabsorbed (ab216772) at a 1/20000 dilution for 1 hour at RT.

All lanes:

Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778)

Predicted band size: 250 kDa

false

• WB

Unknown

Western blot - Anti-Aggrecan antibody [6-B-4] (AB3778)

Buffer and Concentration : 2% BSA/TBST

Exposure time : 180 seconds

All lanes:

Western blot - Anti-Aggrecan antibody [6-B-4] (ab3778) at 1/1000 dilution

All lanes:

C-terminal 10 His tagged Human Aggrecan recombinant protein (aa 20 to 675) 15ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 250 kDa

false