AB216965

Anti-Aggrecan antibody

Name: Anti-Aggrecan antibody (ab216965) | Abcam
Brand: Abcam Limited
SKU: AB216965
Price: 525 USD
Availability: InStock

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(12 Publications )

Rabbit Polyclonal Aggrecan antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human ACAN aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

AGC1, CSPG1, MSK16, ACAN, Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, Chondroitin sulfate proteoglycan 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (AB216965)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (AB216965)

Immunohistochemical analysis of formalin fixed, paraffin embedded Mouse embryo tissue labeling Aggrecan with ab216965 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody (AB216965)

Immunohistochemical analysis of formalin fixed, paraffin embedded Rat synovial tissue labeling Aggrecan with ab216965 at 1/100 dilution, followed by secondary detection and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ACAN aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P16112

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aggrecan also known as aggrecan core protein or chondroitin sulfate proteoglycan 1 is a large proteoglycan vital for cartilage structure and function. It has a mass of approximately 250 kDa. Aggrecan is expressed mostly in cartilage regions such as articular cartilage but it might also be found in other connective tissues. The structure of aggrecan consists of a core protein that attaches to glycosaminoglycan chains allowing it to create hydrated gel structures essential for the resilience and load-bearing properties of cartilage.

Biological function summary

Aggrecan plays a significant role in maintaining the structural integrity of cartilage by providing resistance to compression. This proteoglycan is a central component of the extracellular matrix forming large aggregates with hyaluronic acid and link proteins. These aggregates contribute to the cartilage's osmotic properties helping resist compression during joint movement. Aggrecan's structural properties make it necessary for normal joint function and health.

Pathways

Aggrecan interacts within the cartilage development and maintenance frameworks. Specifically it participates in the regulation of the ECM-receptor interaction pathway and the proteoglycans in cancer pathway. Aggrecan functions alongside proteins such as type II collagen to ensure biomechanical properties of the cartilage. Additionally its enzymatic breakdown by aggrecanases like ADAMTS can impact these pathways leading to various physiological effects.

Aggrecan is closely associated with osteoarthritis and intervertebral disc degeneration. In osteoarthritis the loss of aggrecan through enzymatic degradation leads to reduced joint function and increased pain. The breakdown involves aggrecanases and other matrix metalloproteinases resulting in cartilage degradation. Diseases such as intervertebral disc degeneration also link to the reduced presence or altered structure of aggrecan impacting spine health and mobility.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

See full target information ACAN

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:79 PubMed39844325

2025

Mechanism of hyperbaric oxygen therapy downregulating H-type angiogenesis in subchondral bone of knee osteoarthritis through the PHD2/HIF-1α pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjian Wang,Wen Yu,Yuxin Zhang,Bo Chen,Zhaoxiang Meng

Bone & joint research 13:4-18 PubMed38163445

2024

The CREB1 inhibitor 666-15 maintains cartilage homeostasis and mitigates osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Zhimin Wu,Guoqiang Yan,Shan Li,Yanzhuo Zhang,Guangping Li,Chengai Wu

Science advances 9:eadf4683 PubMed37352350

2023

Optogenetic-induced muscle loading leads to mechanical adaptation of the Achilles tendon enthesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Elahe Ganji,Syeda N Lamia,Matthew Stepanovich,Noelle Whyte,Robert W Goulet,Adam C Abraham,Megan L Killian

Frontiers in pharmacology 14:1159139 PubMed37361231

2023

Intra-articular injection of kartogenin promotes fibrocartilage stem cell chondrogenesis and attenuates temporomandibular joint osteoarthritis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Qiu,Bingqiang Hua,Xiaoping Ye,Xianwen Liu

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 41:2405-2417 PubMed37186383

2023

Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2/NF-κB and SIRT6/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyi Xia,Jian Xiao,ChengLin Tong,Jiajie Lu,Yurong Tu,Sunlong Li,Libing Ni,Yifeng Shi,Peng Luo,Xiaolei Zhang,Xiangyang Wang

Molecular medicine (Cambridge, Mass.) 29:30 PubMed36858954

2023

BMP7 ameliorates intervertebral disc degeneration in type 1 diabetic rats by inhibiting pyroptosis of nucleus pulposus cells and NLRP3 inflammasome activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jun Yu,Ying-Guang Wang,Rui Lu,Xin-Zhen Guo,Yun-Kun Qu,Shan-Xi Wang,Hao-Ran Xu,Hao Kang,Hong-Bo You,Yong Xu

Bioactive materials 21:69-85 PubMed36017070

2022

An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Shun Xia,Dong-Dong Li,Cheng-Gui Wang,Li-Wei Ying,Jing-Kai Wang,Biao Yang,Jia-Wei Shu,Xian-Peng Huang,Yu-Ang Zhang,Chao Yu,Xiao-Peng Zhou,Fang-Cai Li,Nigel K H Slater,Jian-Bin Tang,Qi-Xin Chen,Cheng-Zhen Liang

Experimental and therapeutic medicine 23:337 PubMed35411209

2022

Ketorolac tromethamine alleviates IL-1β-induced chondrocyte injury by inhibiting COX-2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Yizhi Chen

Frontiers in endocrinology 12:749449 PubMed34925230

2021

The Role of Estrogen Receptor α in Response to Longitudinal Bone Growth in ob/ob Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Yu Jin,Chen Guo,Shuai Xu,Hai-Ying Liu,Xin-Feng Li

BioMed research international 2021:3371091 PubMed34395611

2021

A Study on COMP and CTX-II as Molecular Markers for the Diagnosis of Intervertebral Disc Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Duo Qi,Zhong-Han Liu,De-Sheng Wu,Yu-Feng Huang

View all publications

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