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Rabbit Recombinant Monoclonal Aggrecan antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

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Images

Sandwich ELISA - Anti-Aggrecan antibody [EPR28019-8] - BSA and Azide free (Capture) (AB318295), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

Alternative names

AGC1, CSPG1, MSK16, ACAN, Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, Chondroitin sulfate proteoglycan 1

Recommended products

Rabbit Recombinant Monoclonal Aggrecan antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28019-8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab318295 is a BSA and Azide free antibody supplied in an unconjugated
format and it is suitable for sandwich ELISAs to quantify Aggrecan.
The recommended pair for sandwich ELISA is:

Capture: ab318295, Aggrecan Capture Antibody (unconjugated)

Detector: Anti-Aggrecan antibody [EPR28019-18] - BSA and Azide free (Detector) ab318296, Aggrecan Detector Antibody (unconjugated)

The reference range value is 7.81-2000 pg/mL.

The recommended antibody orientation is based on internal optimization
for ELISAbased assays. Antibody orientation is assay dependent and
needs to be optimized for each assay type. Please note that the
range provided for this antibody is only an estimation based on the
performance of the product using the recommended antibody pair.
Performance of the antibody pair will depend on the specific
characteristics of your assay. We guarantee the product works in
sandwich ELISA, but we do not guarantee the sensitivity or dynamic
range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA,
sodium azide and glycerol for higher conjugation efficiency.
The antibodies are provided at an approximate concentration of
1 mg/ml as measured by the protein A280 method. Use our conjugation
kits for antibody conjugates that are ready-to-use in as
little as 20 minutes with <1 minute hands-on-time and 100% antibody
recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aggrecan also known as aggrecan core protein or chondroitin sulfate proteoglycan 1 is a large proteoglycan vital for cartilage structure and function. It has a mass of approximately 250 kDa. Aggrecan is expressed mostly in cartilage regions such as articular cartilage but it might also be found in other connective tissues. The structure of aggrecan consists of a core protein that attaches to glycosaminoglycan chains allowing it to create hydrated gel structures essential for the resilience and load-bearing properties of cartilage.

Biological function summary

Aggrecan plays a significant role in maintaining the structural integrity of cartilage by providing resistance to compression. This proteoglycan is a central component of the extracellular matrix forming large aggregates with hyaluronic acid and link proteins. These aggregates contribute to the cartilage's osmotic properties helping resist compression during joint movement. Aggrecan's structural properties make it necessary for normal joint function and health.

Pathways

Aggrecan interacts within the cartilage development and maintenance frameworks. Specifically it participates in the regulation of the ECM-receptor interaction pathway and the proteoglycans in cancer pathway. Aggrecan functions alongside proteins such as type II collagen to ensure biomechanical properties of the cartilage. Additionally its enzymatic breakdown by aggrecanases like ADAMTS can impact these pathways leading to various physiological effects.

Associated diseases and disorders

Aggrecan is closely associated with osteoarthritis and intervertebral disc degeneration. In osteoarthritis the loss of aggrecan through enzymatic degradation leads to reduced joint function and increased pain. The breakdown involves aggrecanases and other matrix metalloproteinases resulting in cartilage degradation. Diseases such as intervertebral disc degeneration also link to the reduced presence or altered structure of aggrecan impacting spine health and mobility.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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