Anti-Aggrecan antibody [EPR28034-86]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Aggrecan with ab313636 at 1/2000 (0.273 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a ACAN expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab313636 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Aggrecan with ab313636 at 1/2000 (0.273 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse spleen.The section was incubated with ab313636 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Aggrecan with ab313636 at 1/2000 (0.273 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse liver.The section was incubated with ab313636 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Immunohistochemical analysis of paraffin-embedded Mouse cartilage tissue labeling Aggrecan with ab313636 at 1/2000 (0.273 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cartilage.The section was incubated with ab313636 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, spleen. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11425904, 16188468). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-Aggrecan antibody [EPR28034-86] (ab313636) at 1/1000 dilution

Lane 1:

Mouse articar cartilage tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-150 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11425904, 16188468). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 6 seconds

All lanes:

Western blot - Anti-Aggrecan antibody [EPR28034-86] (ab313636) at 1/1000 dilution

Lane 1:

Rat articar cartilage tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50-150 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-Aggrecan antibody [EPR28034-86] (AB313636)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 1 second

All lanes:

Western blot - Anti-Aggrecan antibody [EPR28034-86] (ab313636) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Human ACAN expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 1s