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AB137616

Anti-AGK antibody

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(8 Publications)

Rabbit Polyclonal AGK antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGK aa 1-250.

View Alternative Names

MULK, AGK, hAGK, Multiple substrate lipid kinase, HsMuLK, MuLK, Multi-substrate lipid kinase

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-AGK antibody (AB137616)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AGK antibody (AB137616)

ab137616 at 1/500 dilution staining AGK in methanol fixed Hep3B cells by Immunofluorescence. Lower image costained with Hoerchst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGK antibody (AB137616)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGK antibody (AB137616)

ab137616 at 1/100 dilution, staining AGK in paraffin embedded H1299 xenograft tissue by Immunohistochemistry.

Western blot - Anti-AGK antibody (AB137616)
  • WB

Unknown

Western blot - Anti-AGK antibody (AB137616)

10% SDS PAGE

All lanes:

Western blot - Anti-AGK antibody (ab137616) at 1/500 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Western blot - Anti-AGK antibody (AB137616)
  • WB

Unknown

Western blot - Anti-AGK antibody (AB137616)

10% SDS-PAGE

All lanes:

Western blot - Anti-AGK antibody (ab137616) at 1/1000 dilution

All lanes:

Mouse heart lysate at 50 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AGK aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q53H12

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGK also known as acylglycerol kinase is an important enzyme involved in lipid metabolism. It is responsible for phosphorylating mono- and diacylglycerol to produce phosphatidic acid. The molecular mass of AGK is approximately 45 kDa. This enzyme is expressed in various human tissues with notable expression in tissues like the heart skeletal muscle and pancreas. The presence of AGK in these tissues indicates its significant role in energy metabolism and lipid regulation.
Biological function summary

AGK participates in the biosynthesis of phospholipids important for maintaining cell membrane integrity. It is also part of the TIM22 complex in the mitochondrial inner membrane which is important for the import and assembly of proteins. This role shows that AGK not only influences lipid metabolism but also affects mitochondrial function and structure. Its activity is critical in tissues that require high energy or have a high turnover of membrane lipids.

Pathways

AGK plays a role in both the glycerolipid and phosphatidylinositol signaling pathways. In the glycerolipid pathway AGK acts to regulate the levels of lipid intermediates which are essential for diverse cellular processes. In the phosphatidylinositol pathway AGK indirectly influences processes like cell growth and survival interacting with other proteins such as PI3K and mTOR. This connects AGK to a wider network of signals impacting cellular functions and health.

AGK is linked to mitochondrial disorders particularly Sengers syndrome which affects energy metabolism. Mutations in AGK can lead to problems in the mitochondrial function highlighting its significance in mitochondrial diseases. Furthermore AGK is associated with some cancers as its dysregulation can influence tumor progression by altering lipid signaling. The interaction of AGK with proteins such as BRAF in cancer shows how it can alter cellular proliferation and survival pathways impacting disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed : 15939762). Does not phosphorylate sphingosine (PubMed : 15939762). Phosphorylates ceramide (By similarity). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (By similarity). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed : 28712724, PubMed : 28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed : 28712724, PubMed : 28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed : 28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed : 15939762).
See full target information AGK
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:31 PubMed39820591

2025

Innovative treatment of age-related hearing loss using MSCs and EVs with Apelin.

Applications

Unspecified application

Species

Unspecified reactive species

Shengqun Xu,Dongliang Liu,Fang Zhang,Yuan Tian

Molecular & cellular proteomics : MCP 23:100709 PubMed38154691

2023

Interactome Analysis Identifies the Role of BZW2 in Promoting Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

George Maio,Mike Smith,Ruchika Bhawal,Sheng Zhang,Jeremy M Baskin,Jenny Li,Hening Lin

Cancer research 83:2034-2051 PubMed37078777

2023

ZDHHC2-Mediated AGK Palmitoylation Activates AKT-mTOR Signaling to Reduce Sunitinib Sensitivity in Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Liang Zhu,Pian Liu,Huan Zhang,Feng Guo,Xin Jin

EMBO reports 24:e56282 PubMed37009826

2023

LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Meng,Shaohua Lu,Yu-Ying Li,Li-Ling Hu,Jing Zhang,Yun Cao,Yang Wang,Chris Zhiyi Zhang,Qing-Yu He

Theranostics 12:5537-5550 PubMed35910796

2022

Inhibition of acylglycerol kinase sensitizes DLBCL to venetoclax via upregulation of FOXO1-mediated BCL-2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Na Ning,Si Zhang,Qi Wu,Xun Li,Dong Kuang,Yaqi Duan,Minghui Xia,Huicheng Liu,Junmei Weng,Hongping Ba,Zhaohui Tang,Xiang Cheng,Heng Mei,Liu Huang,Qilin Ao,Guoping Wang,Yu Hu,Arian Laurence,Jing Wang,Guihua Wang,Xiang-Ping Yang

Bioengineered 13:13055-13069 PubMed35635053

2022

Circ_0008068 facilitates the oral squamous cell carcinoma development by microRNA-153-3p/acylgycerol kinase (AGK) axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Long,Chenxing Li,Baoyu Zhu

Cytokine 148:155595 PubMed34116927

2021

Acylglycerol kinase promotes paclitaxel resistance in nasopharyngeal carcinoma cells by regulating FOXM1 via the JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhao,Hui-Ying Chen,Feng Zhao,Hua-Jun Feng,Ji-Ping Su

Environmental toxicology 35:1299-1307 PubMed32652857

2020

Acylglycerol kinase promotes the stemness of nasopharyngeal carcinoma cells by promoting β-catenin translocation to the nucleus through activating PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhao,Peng Sun,Songbing Qin,Jisheng Liu
View all publications
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