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Rabbit Recombinant Monoclonal AGL/Alpha-glucosidase antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (AB230798), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (AB230798), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (AB230798), expandable thumbnail
  • Western blot - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (AB230798), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (AB230798), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

Target data

Function

Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.

Alternative names

GDE, AGL, Glycogen debranching enzyme, Glycogen debrancher

Recommended products

Rabbit Recombinant Monoclonal AGL/Alpha-glucosidase antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8880
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Dissociation constant
6.9 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab230798 is the carrier-free version of Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha-glucosidase also known as AGL is an enzyme that breaks down carbohydrates by hydrolyzing alpha-14 glycosidic bonds in glycogen converting them into glucose molecules. This enzyme is important for the body's ability to derive glucose from stored glycogen a necessary process for maintaining energy levels. AGL is predominantly expressed in liver tissue where it plays an important role in glycogen metabolism. The AGL enzyme has an approximate mass of around 104 kDa. Additionally AGL can be referred to as alpha-16-glucosidase due to its specific hydrolytic capabilities on branching points in glycogen.

Biological function summary

AGL functions as part of the glycogen debranching enzyme complex which is important for glycogen catabolism. It cooperates with other enzymes to effectively mobilize glucose from glycogen stores. AGL's debranching activity ensures the smooth continuation of glycogenolysis providing glucose to meet energy demands during fasting or intense physical activity. This highlights the enzyme's importance in glucose homeostasis and metabolic processes that require rapid energy availability.

Pathways

AGL participates in the glycogenolysis and gluconeogenesis pathways. It partners with phosphorylase to release glucose-1-phosphate from glycogen ensuring energy supply during fasting conditions. The glycogenolysis pathway relies significantly on AGL for breaking down glycogen's branched structures while glucose-6-phosphatase assists in converting glucose-6-phosphate into free glucose. AGL's role in these pathways illustrates its importance in energy metabolism and glucose regulation.

Associated diseases and disorders

AGL's dysfunction is linked to glycogen storage disease type III known as Cori disease. This genetic disorder results from mutations in the AGL gene causing a build-up of abnormally structured glycogen in the liver and muscles leading to symptoms such as hepatomegaly muscular weakness and hypoglycemia. AGL along with other enzymes involved in glycogen metabolism like phosphorylase kinase is a target for therapeutic interventions aimed at managing such glycogen storage diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling AGL/Alpha-glucosidase with purified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 at 1/80 dilution (10�g/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor� 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

  • Immunocytochemistry/ Immunofluorescence - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling AGL/Alpha-glucosidase with Purified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor? 594) 1:200. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling AGL/Alpha-glucosidase with Purified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 at 1:2000 dilution (0.4 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP)
    secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

  • Western blot - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Western blot - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    This IHC data was generated using the same anti-AGL/Alpha-glucosidase antibody clone, EPR8880, in a different buffer formulation (cat# Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: AGL/Alpha-glucosidase knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HepG2 whole cell lysate (20 μg)
    Lane 4: Hek293 whole cell lysate (20 μg
    Lanes 1 - 4: Merged signal (red and green). Green - Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 observed at 170 kDa. Red - loading control, ab18058, observed at 130 kDa.

    Unpurified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 was shown to specifically react with AGL/Alpha-glucosidase when AGL/Alpha-glucosidase knockout samples were used. Wild-type and AGL/Alpha-glucosidase knockout samples were subjected to SDS-PAGE.
    Unpurified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-AGL/Alpha-glucosidase antibody [EPR8880] (Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720)

    Predicted band size: 175 kDa

    Observed band size: 170 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling AGL/Alpha-glucosidase with purified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 at 1/250 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor®488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    This IHC data was generated using the same anti-AGL/Alpha-glucosidase antibody clone, EPR8880, in a different buffer formulation (cat# Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelled with unpurified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 at 1/100 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    Immunohistochemical analysis of paraffin-embedded Human muscle tissue labelled with unpurified Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGL/Alpha-glucosidase antibody [EPR8880] ab133720).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798), expandable thumbnail

    OI-RD Scanning - Anti-AGL/Alpha-glucosidase antibody [EPR8880] - BSA and Azide free (ab230798)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

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