Anti-AGMAT antibody [EPR30229-20]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AGMAT with ab325541 at 1/2000 (0.252 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Mitochondrion staining on human kidney (PMID : 11804860; PMID : 11914032).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma tissue labeling AGMAT with ab325541 at 1/2000 (0.252'μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Mitochondrion staining on human clear cell carcinoma.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGMAT with ab325541 at 1/2000 (0.252 μg/mll) dilution, followed by a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Mitochondrion staining on human liver (PMID : 11804860; PMID : 11914032).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Immunohistochemical analysis of paraffin-embedded Panel A : HEK-293T cells transfected with a human AGMAT expression vector containing a His-tag cell pellet Panel B : HEK-293T cells transfected with an empty vector containing a His-tag cell pellet tissue labeling AGMAT with ab325541 at 1/2000 (0.252μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on 293T cells transfected with a human AGMAT expression vector containing a His-tag (Panel A), negative staining on 293T cells transfected with an empty vector containing a His-tag (Panel B).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling AGMAT with ab325541 at 1/2000 (0.252μg/ml ) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : No staining on human cardiac muscle (PMID : 11804860; PMID : 11914032).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling AGMAT with ab325541 at 1/2000 (0.252 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : No staining on human spleen.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Lab

Western blot - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, testis(PMID : 11804860; PMID : 11914032)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lane 1 : 180 seconds; Lanes 2-4 : 59 seconds

All lanes:

Western blot - Anti-AGMAT antibody [EPR30229-20] (ab325541) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab186735)(1 : 1000)((~16KDa)).

All lanes:

Western blot - Anti-AGMAT antibody [EPR30229-20] (ab325541) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) non-mitochondrial fraction at 20 µg

Lane 2:

HepG2 mitochondria fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,36 kDa,16 kDa

false

Exposure time: 136s

• WB

Lab

Western blot - Anti-AGMAT antibody [EPR30229-20] (AB325541)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Jurkat, U-2 OS

The identity of the bands higher than 30 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lane 1 : 180 seconds; Lanes 2-4 : 59 seconds

All lanes:

Western blot - Anti-AGMAT antibody [EPR30229-20] (ab325541) at 1/1000 dilution

Lane 1:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 4:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,36 kDa

false