JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB323834

Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Multiclonal AGO1 antibody. Suitable for WB and reacts with Transfected cell lysate, Human, Mouse, Rat samples.

View Alternative Names

EIF2C1, AGO1, Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Putative RNA-binding protein Q99, eIF-2C 1, eIF2C 1, EIF2C2, AGO2, Protein argonaute-2, Argonaute2, hAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, eIF-2C 2, eIF2C 2, PPD, EIF2C3, AGO3, Protein argonaute-3, Argonaute3, hAgo3, Argonaute RISC catalytic component 3, Eukaryotic translation initiation factor 2C 3, eIF-2C 3, eIF2C 3, EIF2C4, KIAA1567, AGO4, Protein argonaute-4, Argonaute4, hAgo4, Argonaute RISC catalytic component 4, Eukaryotic translation initiation factor 2C 4, eIF-2C 4, eIF2C 4, Eif2c2, Kiaa4215, Protein argonaute-2, Argonaute2, mAgo2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, Piwi/argonaute family protein meIF2C2, Protein slicer, eIF-2C 2, eIF2C 2, Eif2c2, Protein argonaute-2, Argonaute2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, Golgi ER protein 95 kDa, Protein slicer, eIF-2C 2, eIF2C 2, GERp95, Eif2c3, Protein argonaute-3, Argonaute3, mAgo3, Argonaute RISC catalytic component 3, Eukaryotic translation initiation factor 2C 3, Piwi/argonaute family protein meIF2C3, eIF-2C 3, eIF2C 3, Eif2c1, Protein argonaute-1, Argonaute1, mAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Piwi/argonaute family protein meIF2C1, eIF-2C 1, eIF2C 1, Eif2c4, Kiaa1567, Protein argonaute-4, Argonaute4, mAgo4, Argonaute RISC catalytic component 4, Eukaryotic translation initiation factor 2C 4, Piwi/argonaute family protein meIF2C4, eIF-2C 4, eIF2C 4

3 Images
Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)
  • WB

Supplier Data

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1-4 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 and lanes 5-7 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000.

Exposure time : Lanes 1-4 : 81 seconds; Lanes 5-9 : 180 seconds

All lanes:

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Human spleen tissue lysate at 20 µg

Lane 6:

Human colon tissue lysate at 20 µg

Lane 7:

Human lung tissue lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 5 - 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 97 kDa

false

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)
  • WB

Supplier Data

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Mouse kidney tissue lysate at 20 µg

Lane 8:

Mouse spleen tissue lysate at 20 µg

Lane 9:

Rat brain tissue lysate at 20 µg

Lane 10:

Rat kidney tissue lysate at 20 µg

Lane 11:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 97 kDa

false

Exposure time: 180s

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)
  • WB

Supplier Data

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-GST antibody [EPR4236] (ab111947) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag® whole cell lysate at 10 µg

Lane 2:

293T transfected with AGO1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T transfected with AGO2 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 4:

293T transfected with AGO3 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 5:

293T transfected with AGO4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa,36 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1196

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
See full target information AGO1

Additional targets

AGO2,AGO3,AGO4,,,,,,,,
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com