Rabbit Recombinant Multiclonal AGO1 antibody. Suitable for WB and reacts with Transfected cell lysate, Human, Mouse, Rat samples.
Images
![Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834), expandable thumbnail](https://content.abcam.com/products/images/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834--western-blot-img446175.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)")
![Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834), expandable thumbnail](https://content.abcam.com/products/images/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834--western-blot-img446174.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)")
![Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834), expandable thumbnail](https://content.abcam.com/products/images/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834--western-blot-img446173.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (AB323834)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | ICC/IF | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell lysate | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell lysate | Dilution info - | Notes - |
Target data
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
Additional Targets
AGO2, AGO3, AGO4, Ago1, Ago2, Ago3, Ago4, AGO1_RAT, Ago2, AGO3_RAT, AGO4_RAT
Alternative names
EIF2C1, AGO1, Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Putative RNA-binding protein Q99, eIF-2C 1, eIF2C 1
Rabbit Recombinant Multiclonal AGO1 antibody. Suitable for WB and reacts with Transfected cell lysate, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- RM1196
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834), expandable thumbnail](https://content.abcam.com/products/images/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834--western-blot-img446175.jpg "Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834)")
Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834)
AGO1 + AGO2 + AGO3 + AGO4 Western blot staining using rabbit Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 6: Mouse brain tissue lysate at 20 µg
Lane 7: Mouse kidney tissue lysate at 20 µg
Lane 8: Mouse spleen tissue lysate at 20 µg
Lane 9: Rat brain tissue lysate at 20 µg
Lane 10: Rat kidney tissue lysate at 20 µg
Lane 11: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 97 kDa
Exposure time: 180s
![Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834), expandable thumbnail](https://content.abcam.com/products/images/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834--western-blot-img446174.jpg "Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834)")
Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834)
AGO1 + AGO2 + AGO3 + AGO4 Western blot staining using rabbit Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lanes 1-4 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 and lanes 5-7 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000.
Exposure time: Lanes 1-4: 81 seconds; Lanes 5-9: 180 seconds
All lanes: Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 4: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: Human spleen tissue lysate at 20 µg
Lane 6: Human colon tissue lysate at 20 µg
Lane 7: Human lung tissue lysate at 20 µg
SecondaryLanes 1 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Lanes 5 - 7: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 97 kDa
![Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834), expandable thumbnail](https://content.abcam.com/products/images/ago1-ago2-ago3-ago4-antibody-rm1196-ab323834--western-blot-img446173.jpg "Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834)")
Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834)
AGO1 + AGO2 + AGO3 + AGO4 Western blot staining using rabbit Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
In Western blot, Anti-GST antibody [EPR4236] (Anti-GST antibody [EPR4236] ab111947) staining at 1/1000 dilution.
All lanes: Western blot - Anti-AGO1 + AGO2 + AGO3 + AGO4 antibody [RM1196] (ab323834) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector (vector control) containing a myc-His-tag® whole cell lysate at 10 µg
Lane 2: 293T transfected with AGO1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 3: 293T transfected with AGO2 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 4: 293T transfected with AGO3 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 5: 293T transfected with AGO4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 125 kDa, 36 kDa
Exposure time: 6s
Downloads
Product protocols
- Visit the general protocols
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