Rabbit Polyclonal AGO1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Drosophila melanogaster samples. Cited in 53 publications.
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Publications
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- iScience 26:1078372023Nuclear miR-150 enhances hepatic lipid accumulation by targeting RNA transcripts overlapping the promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiao Luo,Yanan Ji,Ningning Chen,Ge Song,Shuyue Zhou,Xuan Niu,Dianke YuPubMed 37736048
- Nature communications 14:21082023Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeike Sheng,Lu Li,Tianqi Li,Yuzhi Wang,Nicholas M Hiers,Jennifer S Mejia,Jossie S Sanchez,Lei Zhou,Mingyi XiePubMed 37055443
- Molecular systems biology 19:e111482023Stress-sensitive dynamics of miRNAs and Elba1 in Drosophila embryogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLovisa Örkenby,Signe Skog,Helen Ekman,Alessandro Gozzo,Unn Kugelberg,Rashmi Ramesh,Srivathsa Magadi,Gianluca Zambanini,Anna Nordin,Claudio Cantú,Daniel Nätt,Anita ÖstPubMed 36938679
- Molecular cell 82:3872-3884.e92022Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena R Kingston,Lianne W Blodgett,David P BartelPubMed 36150386
- STAR protocols 3:1015962022Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYajie Gao,Yuanxiang Zhu,Qinmiao Sun,Dahua ChenPubMed 35928005
- Frontiers in cell and developmental biology 10:8798772022Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis the Transfer of let-7f-5p and miR-210-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanpan Liu,Lihong Qin,Chang Liu,Jun Mi,Qun Zhang,Shuangshuang Wang,Dexuan Zhuang,Qiuping Xu,Wenqian Chen,Jing Guo,Xunwei WuPubMed 35557954
- Frontiers in molecular biosciences 9:8183022022The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Xenia Lim,Wakana Isshiki,Taichiro Iki,Shinichi Kawaguchi,Toshie KaiPubMed 35425810
- RNA (New York, N.Y.) 27:710-7242021Ago2 protects siRNAs and microRNAs from target-directed degradation, even in the absence of 2'--methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena R Kingston,David P BartelPubMed 33853897
- Cell host & microbe 29:834-848.e132021Argonaute-CLIP delineates versatile, functional RNAi networks in Aedes aegypti, a major vector of human viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryn Rozen-Gagnon,Meigang Gu,Joseph M Luna,Ji-Dung Luo,Soon Yi,Sasha Novack,Eliana Jacobson,Wei Wang,Matthew R Paul,Troels K H Scheel,Thomas Carroll,Charles M RicePubMed 33794184
- Scientific reports 11:7362021A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathryn Rozen-Gagnon et. al.PubMed 33436886
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Mouse | Expected | Predicted | Expected |
| Drosophila melanogaster | Expected | Tested | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Drosophila melanogaster | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
Alternative names
EIF2C1, AGO1, Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Putative RNA-binding protein Q99, eIF-2C 1, eIF2C 1
Recommended products
Rabbit Polyclonal AGO1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Drosophila melanogaster samples. Cited in 53 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Argonaute 1 (Ago1) also known as "agoone" is a protein that plays a central role in RNA silencing processes. It weighs around 97 kDa and is a component of the RNA-induced silencing complex (RISC). Ago1 is expressed in various tissues where it binds to small RNAs such as microRNAs (miRNAs) and small interfering RNAs (siRNAs) to guide these molecules in targeting complementary RNA sequences for gene silencing. Ago1's ability to slice or inhibit translation of target RNAs makes it important in gene expression regulation.
- Biological function summary
Ago1 is essential in the regulation of gene expression at the post-transcriptional level. As part of the RISC complex it binds to small regulatory RNAs facilitating their role in either cleaving target RNAs or repressing their translation. This function affects numerous cellular processes including cell differentiation proliferation and development. Ago1 also interacts with other Argonaute family members like Ago2 which is directly involved in RNA cleavage. Together these proteins ensure precise regulation of gene expression.
- Pathways
The functional activities of Ago1 are significant in the RNA interference (RNAi) and microRNA (miRNA) pathways. In the RNAi pathway Ago1 participates by associating with siRNAs to silence target genes. In the miRNA pathway it works with miRNAs to repress translation or degrade mRNA transcripts. Ago1 collaborates with proteins like Dicer which assists in processing miRNAs and siRNAs highlighting its integrated role within these regulatory systems.
- Associated diseases and disorders
Disruptions in Ago1 activity have implications in cancers and neurodegenerative diseases. In cancer altered expression of Ago1 can lead to improper regulation of gene silencing affecting tumor suppressor genes and oncogenes. In neurodegenerative diseases the dysregulation of miRNA pathways mediated by Ago1 links it to conditions like Alzheimer's disease. Other proteins like Ago2 also play roles in these diseases often working in concert with Ago1 to maintain cellular homeostasis and prevent disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Ago1 antibody (ab5070)
All lanes: Western blot - Anti-Ago1 antibody (ab5070) at 1 µg/mL
All lanes: Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDa, 48 kDa
Exposure time: 30s
This image is courtesy of an anonymous customer reviewWestern blot - Anti-Ago1 antibody (ab5070)
Blot was blocked with 5% Milk for 1 hour at room temperature.
All lanes: Western blot - Anti-Ago1 antibody (ab5070)
All lanes: Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
SecondaryAll lanes: Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDa
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