AB5070

Anti-Ago1 antibody

Name: Anti-Ago1 antibody (ab5070) | Abcam
Brand: Abcam Limited
SKU: AB5070
Price: 450 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(57 Publications)

Rabbit Polyclonal AGO1 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Drosophila melanogaster samples. Cited in 57 publications.

View Alternative Names

EIF2C1, AGO1, Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Putative RNA-binding protein Q99, eIF-2C 1, eIF2C 1

2 Images

AbReview29654****

Western blot - Anti-Ago1 antibody (AB5070)

Blot was blocked with 5% Milk for 1 hour at room temperature.

All lanes:

Western blot - Anti-Ago1 antibody (ab5070) at 1/500 dilution

All lanes:

Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg

Secondary

All lanes:

Sheep anti-rabbit HRP conjugated IgG Polyclonal at 1/2000 dilution

Predicted band size: 97 kDa

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This image is courtesy of an anonymous Abreview

Lab

Western blot - Anti-Ago1 antibody (AB5070)

All lanes:

Western blot - Anti-Ago1 antibody (ab5070) at 1 µg/mL

All lanes:

Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 110 kDa,48 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster, Mouse

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Argonaute 1 (Ago1) also known as "agoone" is a protein that plays a central role in RNA silencing processes. It weighs around 97 kDa and is a component of the RNA-induced silencing complex (RISC). Ago1 is expressed in various tissues where it binds to small RNAs such as microRNAs (miRNAs) and small interfering RNAs (siRNAs) to guide these molecules in targeting complementary RNA sequences for gene silencing. Ago1's ability to slice or inhibit translation of target RNAs makes it important in gene expression regulation.

Biological function summary

Ago1 is essential in the regulation of gene expression at the post-transcriptional level. As part of the RISC complex it binds to small regulatory RNAs facilitating their role in either cleaving target RNAs or repressing their translation. This function affects numerous cellular processes including cell differentiation proliferation and development. Ago1 also interacts with other Argonaute family members like Ago2 which is directly involved in RNA cleavage. Together these proteins ensure precise regulation of gene expression.

Pathways

The functional activities of Ago1 are significant in the RNA interference (RNAi) and microRNA (miRNA) pathways. In the RNAi pathway Ago1 participates by associating with siRNAs to silence target genes. In the miRNA pathway it works with miRNAs to repress translation or degrade mRNA transcripts. Ago1 collaborates with proteins like Dicer which assists in processing miRNAs and siRNAs highlighting its integrated role within these regulatory systems.

Disruptions in Ago1 activity have implications in cancers and neurodegenerative diseases. In cancer altered expression of Ago1 can lead to improper regulation of gene silencing affecting tumor suppressor genes and oncogenes. In neurodegenerative diseases the dysregulation of miRNA pathways mediated by Ago1 links it to conditions like Alzheimer's disease. Other proteins like Ago2 also play roles in these diseases often working in concert with Ago1 to maintain cellular homeostasis and prevent disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

See full target information AGO1

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113272 PubMed40894916

2025

Gene expression dynamics before and after zygotic gene activation in early embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongwoo Na,Yeon Choi,Thi Thanh My Nguyen,Hoang-Anh Pham-Bui,Jeesoo Kim,V Narry Kim,Mihye Lee,Jong-Seo Kim

Genome research 33:2119-2132 PubMed37973194

2023

tRNA-derived small RNAs are embedded in the gene regulatory network instructing metamorphosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junling Shi,Jiaqi Xu,Jun Ma,Feng He

iScience 26:107837 PubMed37736048

2023

Nuclear miR-150 enhances hepatic lipid accumulation by targeting RNA transcripts overlapping the promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Luo,Yanan Ji,Ningning Chen,Ge Song,Shuyue Zhou,Xuan Niu,Dianke Yu

Nature communications 14:2108 PubMed37055443

2023

Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999.

Applications

Unspecified application

Species

Unspecified reactive species

Peike Sheng,Lu Li,Tianqi Li,Yuzhi Wang,Nicholas M Hiers,Jennifer S Mejia,Jossie S Sanchez,Lei Zhou,Mingyi Xie

Molecular systems biology 19:e11148 PubMed36938679

2023

Stress-sensitive dynamics of miRNAs and Elba1 in Drosophila embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lovisa Örkenby,Signe Skog,Helen Ekman,Alessandro Gozzo,Unn Kugelberg,Rashmi Ramesh,Srivathsa Magadi,Gianluca Zambanini,Anna Nordin,Claudio Cantú,Daniel Nätt,Anita Öst

Molecular cell 82:3872-3884.e9 PubMed36150386

2022

Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development.

Applications

Unspecified application

Species

Unspecified reactive species

Elena R Kingston,Lianne W Blodgett,David P Bartel

STAR protocols 3:101596 PubMed35928005

2022

Optimized protocols for RNA-induced silencing complex assembly and cleavage in cultured cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yajie Gao,Yuanxiang Zhu,Qinmiao Sun,Dahua Chen

Frontiers in cell and developmental biology 10:879877 PubMed35557954

2022

Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis the Transfer of let-7f-5p and miR-210-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Panpan Liu,Lihong Qin,Chang Liu,Jun Mi,Qun Zhang,Shuangshuang Wang,Dexuan Zhuang,Qiuping Xu,Wenqian Chen,Jing Guo,Xunwei Wu

Frontiers in molecular biosciences 9:818302 PubMed35425810

2022

The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis in .

Applications

Unspecified application

Species

Unspecified reactive species

Lin-Xenia Lim,Wakana Isshiki,Taichiro Iki,Shinichi Kawaguchi,Toshie Kai

RNA (New York, N.Y.) 27:710-724 PubMed33853897

2021

Ago2 protects siRNAs and microRNAs from target-directed degradation, even in the absence of 2'--methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Elena R Kingston,David P Bartel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ago1 antibody

Western blot - Anti-Ago1 antibody (AB5070)

Western blot - Anti-Ago1 antibody (AB5070)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (57)

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