Rabbit Monoclonal AGO1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--immunoprecipitation-img176903.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)")
![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--immunoprecipitation-img165734.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)")
![Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--western-blot-img173422.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)")
![Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--western-blot-img169661.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | Flow Cyt (Intra) | ChIP | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Expected | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
Alternative names
EIF2C1, AGO1, Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Putative RNA-binding protein Q99, eIF-2C 1, eIF2C 1
Recommended products
Rabbit Monoclonal AGO1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25441-8
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Argonaute 1 (Ago1) also known as "agoone" is a protein that plays a central role in RNA silencing processes. It weighs around 97 kDa and is a component of the RNA-induced silencing complex (RISC). Ago1 is expressed in various tissues where it binds to small RNAs such as microRNAs (miRNAs) and small interfering RNAs (siRNAs) to guide these molecules in targeting complementary RNA sequences for gene silencing. Ago1's ability to slice or inhibit translation of target RNAs makes it important in gene expression regulation.
- Biological function summary
Ago1 is essential in the regulation of gene expression at the post-transcriptional level. As part of the RISC complex it binds to small regulatory RNAs facilitating their role in either cleaving target RNAs or repressing their translation. This function affects numerous cellular processes including cell differentiation proliferation and development. Ago1 also interacts with other Argonaute family members like Ago2 which is directly involved in RNA cleavage. Together these proteins ensure precise regulation of gene expression.
- Pathways
The functional activities of Ago1 are significant in the RNA interference (RNAi) and microRNA (miRNA) pathways. In the RNAi pathway Ago1 participates by associating with siRNAs to silence target genes. In the miRNA pathway it works with miRNAs to repress translation or degrade mRNA transcripts. Ago1 collaborates with proteins like Dicer which assists in processing miRNAs and siRNAs highlighting its integrated role within these regulatory systems.
- Associated diseases and disorders
Disruptions in Ago1 activity have implications in cancers and neurodegenerative diseases. In cancer altered expression of Ago1 can lead to improper regulation of gene silencing affecting tumor suppressor genes and oncogenes. In neurodegenerative diseases the dysregulation of miRNA pathways mediated by Ago1 links it to conditions like Alzheimer's disease. Other proteins like Ago2 also play roles in these diseases often working in concert with Ago1 to maintain cellular homeostasis and prevent disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--immunoprecipitation-img176903.jpg "Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)")
Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)
Ago1 was immunoprecipitated from mouse lung tissue lysate 10 μg with Anti-Ago1 antibody [EPR25441-8] ab300152 at 1/30 dilution. VeriBlot for IP Detection Reagent (HRP) ab131366 was used as a secondary antibody at 1/5000 dilution.
Lane 1(Input)
Mouse lung tissue lysate 10 μg
Lane 2(+)
Mouse lung tissue lysate
Lane 3(-)
Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Ago1 antibody [EPR25441-8] ab300152 in mouse lung tissue lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (Anti-Ago1 antibody [EPR25441-8] ab300152)
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--immunoprecipitation-img165734.jpg "Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)")
Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)
This data was developed using Anti-Ago1 antibody [EPR25441-8] ab300152, the same antibody clone in a different buffer formulation.
Ago1 was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with Anti-Ago1 antibody [EPR25441-8] ab300152 at 1/30 dilution. VeriBlot for IP Detection Reagent (HRP) ab131366 was used as a secondary antibody at 1/5000 dilution.
Lane 1(Input)
HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2(+)
HeLa whole cell lysate
Lane 3(-)
Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Ago1 antibody [EPR25441-8] ab300152 in HeLa whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (Anti-Ago1 antibody [EPR25441-8] ab300152)
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 3min
![Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--western-blot-img173422.jpg "Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)")
Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)
This data was developed using Anti-Ago1 antibody [EPR25441-8] ab300152, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time:
Lane 1: 3 minutes
Lane 2: 92 seconds
Lane 3: 15 seconds
Lane 4: 37 secondsThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487329).
The identity of the lower MW band at approximately 37kDa is unknown.All lanes: Western blot - Anti-Ago1 antibody [EPR25441-8] (Anti-Ago1 antibody [EPR25441-8] ab300152) at 1/1000 dilution
Lane 1: Human lung tissue lysate at 20 µg
Lane 2: Mouse lung tissue lysate at 20 µg
Lane 3: Mouse heart tissue lysate at 20 µg
Lane 4: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
![Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153), expandable thumbnail](https://content.abcam.com/products/images/ago1-antibody-epr25441-8-bsa-and-azide-free-ab300153--western-blot-img169661.jpg "Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)")
Western blot - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (ab300153)
This data was developed using Anti-Ago1 antibody [EPR25441-8] ab300152, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 29487329).
All lanes: Western blot - Anti-Ago1 antibody [EPR25441-8] (Anti-Ago1 antibody [EPR25441-8] ab300152) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA1 specifically targeting AGO1, whole cell lysate at 20 µg
Lane 3: HeLa transfected with siRNA2 specifically targeting AGO1, whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Exposure time: 3min
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