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AB279392

Anti-Ago1 antibody [OTI4G1]

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(2 Publications)

Mouse Monoclonal AGO1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGO1 aa 200-500.

View Alternative Names

EIF2C1, AGO1, Protein argonaute-1, Argonaute1, hAgo1, Argonaute RISC catalytic component 1, Eukaryotic translation initiation factor 2C 1, Putative RNA-binding protein Q99, eIF-2C 1, eIF2C 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (AB279392)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (AB279392)

Paraffin embedded human embryonic cerebellum tissue stained for AGO1 using ab279392 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer, pH 8.5, at 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (AB279392)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (AB279392)

Paraffin embedded human placenta tissue stained for AGO1 using ab279392 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer, pH8.5, at 120°C for 3 minutes.

Western blot - Anti-Ago1 antibody [OTI4G1] (AB279392)
  • WB

Supplier Data

Western blot - Anti-Ago1 antibody [OTI4G1] (AB279392)

All lanes:

Western blot - Anti-Ago1 antibody [OTI4G1] (ab279392) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg

Lane 2:

Human brain tissue lysate at 35 µg

Predicted band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4G1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AGO1 aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

Q9UL18

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Argonaute 1 (Ago1) also known as "agoone" is a protein that plays a central role in RNA silencing processes. It weighs around 97 kDa and is a component of the RNA-induced silencing complex (RISC). Ago1 is expressed in various tissues where it binds to small RNAs such as microRNAs (miRNAs) and small interfering RNAs (siRNAs) to guide these molecules in targeting complementary RNA sequences for gene silencing. Ago1's ability to slice or inhibit translation of target RNAs makes it important in gene expression regulation.
Biological function summary

Ago1 is essential in the regulation of gene expression at the post-transcriptional level. As part of the RISC complex it binds to small regulatory RNAs facilitating their role in either cleaving target RNAs or repressing their translation. This function affects numerous cellular processes including cell differentiation proliferation and development. Ago1 also interacts with other Argonaute family members like Ago2 which is directly involved in RNA cleavage. Together these proteins ensure precise regulation of gene expression.

Pathways

The functional activities of Ago1 are significant in the RNA interference (RNAi) and microRNA (miRNA) pathways. In the RNAi pathway Ago1 participates by associating with siRNAs to silence target genes. In the miRNA pathway it works with miRNAs to repress translation or degrade mRNA transcripts. Ago1 collaborates with proteins like Dicer which assists in processing miRNAs and siRNAs highlighting its integrated role within these regulatory systems.

Disruptions in Ago1 activity have implications in cancers and neurodegenerative diseases. In cancer altered expression of Ago1 can lead to improper regulation of gene silencing affecting tumor suppressor genes and oncogenes. In neurodegenerative diseases the dysregulation of miRNA pathways mediated by Ago1 links it to conditions like Alzheimer's disease. Other proteins like Ago2 also play roles in these diseases often working in concert with Ago1 to maintain cellular homeostasis and prevent disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).
See full target information AGO1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 48: PubMed36222298

2022

Glutathione peroxidase 2 overexpression promotes malignant progression and cisplatin resistance of KRAS‑mutated lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Wang,Xu Chen,Guang Fu,Mingjian Ge

International journal of oncology 59: PubMed34278448

2021

Exosomal transfer of miR‑25‑3p promotes the proliferation and temozolomide resistance of glioblastoma cells by targeting FBXW7.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxin Wang,Tianxiao Li,Bin Wang
View all publications
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Product promise

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