AB154844

Anti-AGO3 antibody [EPR9576]

RabMAb
Recombinant
KO Validated
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal AGO3 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

EIF2C3, AGO3, Protein argonaute-3, Argonaute3, hAgo3, Argonaute RISC catalytic component 3, Eukaryotic translation initiation factor 2C 3, eIF-2C 3, eIF2C 3

2 Images

Lab

Western blot - Anti-AGO3 antibody [EPR9576] (AB154844)

ab154844 Anti-AGO3 antibody [EPR9576] was shown to specifically react with Argonaute RISC Catalytic Component 3 in HeLa wild-type cells (ab255928). Loss of signal was observed when knockout cell line ab265320 (knockout cell lysate ab257819) was used. Wild-type and Argonaute RISC Catalytic Component 3 knockout samples were subjected to SDS-PAGE. ab154844 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Western blot - Anti-AGO3 antibody [EPR9576] (ab154844) at 1/2000 dilution

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154844 at 1/2000 dilution

Lane 1:

Wild-type HeLa lysate at 40 µg

Lane 2:

Argonaute RISC Catalytic Component 3 knockout HeLa lysate at 40 µg

Lane 2:

Western blot - Human AGO3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ago3-knockout-hela-cell-line-ab265320'>ab265320</a>)

Lane 3:

Daudi lysate at 40 µg

Lane 4:

A549 lysate at 40 µg

Predicted band size: 97 kDa

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Western blot - Anti-AGO3 antibody [EPR9576] (AB154844)

All lanes:

Western blot - Anti-AGO3 antibody [EPR9576] (ab154844) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µL/mL Buffer

Lane 2:

Human fetal kidney lysate at 10 µL/mL Buffer

Predicted band size: 97 kDa

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Carrier free

Anti-AGO3 antibody [EPR9576] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9576

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGO3 also known as Argonaute protein 3 is an important component of the RNA-induced silencing complex (RISC). It is part of the Argonaute family which plays a role in RNA silencing processes. AGO3 usually has a molecular mass of around 97 kDa. It is mainly expressed in human tissues such as testes and is responsible for processing small non-coding RNAs including miRNAs and siRNAs. These small RNAs guide AGO3 to complementary RNA sequences facilitating the cleavage and degradation of target mRNA.

Biological function summary

AGO3 contributes to gene expression regulation by silencing specific RNA transcripts. This protein forms a complex with other proteins and RNA molecules to execute its functions. Part of the miRNA and RNA interference pathways AGO3 associates with Dicer and other Argonaute proteins creating an effective silencing machinery that modulates various cellular processes. Its activity is essential for maintaining cellular homeostasis through RNA interference-mediated gene silencing.

Pathways

AGO3 plays a significant role in the RNA interference (RNAi) and the microRNA (miRNA) pathways. These pathways are key in post-transcriptional gene regulation controlling gene expression patterns important for development and differentiation. AGO3 interacts with Dicer a pivotal enzyme in the RNAi pathway facilitating the processing of precursor miRNAs into mature miRNAs further guiding RISC to its target mRNA sequences. These interactions highlight AGO3's critical place in RNA silencing networks.

AGO3 has links to certain cancers and hereditary disorders. Aberrations in its expression or function can impact miRNA-mediated regulation contributing to tumorigenesis and disorders related to gene expression imbalances. For instance AGO3's dysfunction can relate to testicular cancer due to its significant expression in testicular tissue. Connections between AGO3 and other Argonaute family proteins like AGO1 and AGO2 may influence disease development highlighting its importance in understanding disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (PubMed : 29040713).

See full target information AGO3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American So 25:2705-2714 PubMed28882451

2017

Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jon Voutila,Vikash Reebye,Thomas C Roberts,Pantelitsa Protopapa,Pinelopi Andrikakou,David C Blakey,Robert Habib,Hans Huber,Pal Saetrom,John J Rossi,Nagy A Habib

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AGO3 antibody [EPR9576]

Western blot - Anti-AGO3 antibody [EPR9576] (AB154844)

Western blot - Anti-AGO3 antibody [EPR9576] (AB154844)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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