JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB85077

Anti-AGO4 antibody

5

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal AGO4 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human AGO4.

View Alternative Names

EIF2C4, KIAA1567, AGO4, Protein argonaute-4, Argonaute4, hAgo4, Argonaute RISC catalytic component 4, Eukaryotic translation initiation factor 2C 4, eIF-2C 4, eIF2C 4

1 Images
Western blot - Anti-AGO4 antibody (AB85077)
  • WB

Unknown

Western blot - Anti-AGO4 antibody (AB85077)

All lanes:

Western blot - Anti-AGO4 antibody (ab85077) at 1/500 dilution

All lanes:

HeLa cell lysate

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AGO4. The exact immunogen used to generate this antibody is proprietary information.

Q9HCK5

style
left-column

Complementary Products

Primary Antibodies

AB154844

Anti-AGO3 antibody [EPR9576]

RabMAb|Recombinant|KO Validated

Western blot - Anti-AGO3 antibody [EPR9576] (AB154844)

  • 0
  • 0
Primary Antibodies

AB88904

Anti-CYP2C8 antibody

Western blot - Anti-CYP2C8 antibody (AB88904)

  • 0
  • 0
Primary Antibodies

AB76901

Anti-ZCCHC6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (AB76901)

  • 0
  • 0
Primary Antibodies

AB109591

Anti-DDX55 antibody

Immunoprecipitation - Anti-DDX55 antibody (AB109591)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Argonaute family member AGO4 also known as Argonaute RISC catalytic component 4 has a significant role in RNA-induced silencing complex (RISC) functions. AGO4's mass is approximately 95 kDa and it is primarily expressed in different tissues including the testes and placenta. AGO4 has been studied regarding its involvement in guiding microRNAs and short interfering RNAs in gene silencing through RNA interference.
Biological function summary

AGO4 participates in small RNA pathways. It is part of the RNA-induced silencing complex and works closely with DICER and TRBP to process and guide small RNA molecules. This process affects cellular mRNA levels by reducing the translation of target mRNA molecules helping regulate gene expression post-transcriptionally.

Pathways

AGO4 functions in the RNA interference and gene silencing by microRNAs pathways affecting post-transcriptional gene regulation. AGO4 interacts with proteins like DICER1 and other Argonaute proteins such as AGO1-3 within these pathways aiding in the effective downregulation of specific mRNA targets.

AGO4 has a connection to cancer and viral infections. Abnormalities in AGO4 expression or function may lead to disruption in gene silencing mechanisms which can contribute to tumor progression. Studies also link AGO4 to interactions with viral proteins highlighting its potential impact on viral replication and pathogenesis.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
See full target information AGO4
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 92:845-858 PubMed29067470

2017

Multiple microRNAs function as self-protective modules in acetaminophen-induced hepatotoxicity in humans.

Applications

EMSA

Species

Human

Dianke Yu,Leihong Wu,Pritmohinder Gill,William H Tolleson,Si Chen,Jinchun Sun,Bridgett Knox,Yaqiong Jin,Wenming Xiao,Huixiao Hong,Yong Wang,Zhen Ren,Lei Guo,Nan Mei,Yongli Guo,Xi Yang,Leming Shi,Yinting Chen,Linjuan Zeng,Kostiantyn Dreval,Volodymyr Tryndyak,Igor Pogribny,Hong Fang,Tieliu Shi,Sandra McCullough,Sudeepa Bhattacharyya,Laura Schnackenberg,William Mattes,Richard D Beger,Laura James,Weida Tong,Baitang Ning

Molecular therapy : the journal of the American So 25:2705-2714 PubMed28882451

2017

Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jon Voutila,Vikash Reebye,Thomas C Roberts,Pantelitsa Protopapa,Pinelopi Andrikakou,David C Blakey,Robert Habib,Hans Huber,Pal Saetrom,John J Rossi,Nagy A Habib

Nucleic acids research 43:10474-91 PubMed26405199

2015

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Applications

WB

Species

Unspecified reactive species

Fudi Zhong,Nan Zhou,Kang Wu,Yubiao Guo,Weiping Tan,Hong Zhang,Xue Zhang,Guannan Geng,Ting Pan,Haihua Luo,Yijun Zhang,Zhibin Xu,Jun Liu,Bingfeng Liu,Wenchao Gao,Chao Liu,Liangliang Ren,Jun Li,Jie Zhou,Hui Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com