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AB235328

Anti-AGPAT1 antibody

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(1 Publication)

Rabbit Polyclonal AGPAT1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGPAT1 aa 200 to C-terminus.

View Alternative Names

G15, AGPAT1, 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha, 1-acylglycerol-3-phosphate O-acyltransferase 1, Lysophosphatidic acid acyltransferase alpha, Protein G15, 1-AGP acyltransferase 1, 1-AGPAT 1, LPAAT-alpha

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT1 antibody (AB235328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT1 antibody (AB235328)

Paraffin-embedded human kidney tissue stained for AGPAT1 with ab235328 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT1 antibody (AB235328)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT1 antibody (AB235328)

Paraffin-embedded human placenta tissue stained for AGPAT1 with ab235328 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-AGPAT1 antibody (AB235328)
  • WB

Supplier Data

Western blot - Anti-AGPAT1 antibody (AB235328)

All lanes:

Western blot - Anti-AGPAT1 antibody (ab235328) at 1/200 dilution

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AGPAT1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99943

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPAT1 also known as 1-acylglycerol-3-phosphate O-acyltransferase 1 performs an important role in lipid biosynthesis. It catalyzes the conversion of lysophosphatidic acid into phosphatidic acid a precursor for glycerolipid synthesis. The molecular mass of AGPAT1 is approximately 28 kDa. AGPAT1 expresses widely in human tissues with higher levels seen in adipose tissue liver and heart.
Biological function summary

AGPAT1 impacts lipid metabolism and energy homeostasis. It participates as a single enzyme and is not typically part of larger protein complexes. By facilitating the production of phosphatidic acid it influences the balance of lipid intermediates necessary for membrane and lipoprotein synthesis. This activity affects cellular processes like membrane dynamics and signal transduction.

Pathways

AGPAT1 plays an integral role in the glycerolipid metabolism pathway. It collaborates with enzymes like lipin-1 to regulate pathways that control lipid storage and release. Its action supports the phosphatidic acid pathway which connects to various cellular signaling cascades. AGPAT1 cooperates with other proteins that modulate lipid signals bringing about effects on cell proliferation and survival.

AGPAT1 associates notably with metabolic disorders and cardiovascular diseases. Dysregulation of AGPAT1 has implications for conditions like obesity and atherosclerosis as improper lipid metabolism can lead to excess lipid accumulation or energy imbalance. AGPAT1's interaction with proteins such as lipin-1 influences these disorders where lipid imbalance and chronic inflammation become important factors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
See full target information AGPAT1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 10:644 PubMed31501420

2019

CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Liu,Xiao Zhang,Jiafei Lin,Yuxin Chen,Yongxia Qiao,Susu Guo,Yueyue Yang,Guoqing Zhu,Qiuhui Pan,Jiayi Wang,Fenyong Sun
View all publications
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