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AB67018

Anti-AGPAT1 antibody

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(3 Publications)

Mouse Polyclonal AGPAT1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AGPAT1.

View Alternative Names

G15, AGPAT1, 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha, 1-acylglycerol-3-phosphate O-acyltransferase 1, Lysophosphatidic acid acyltransferase alpha, Protein G15, 1-AGP acyltransferase 1, 1-AGPAT 1, LPAAT-alpha

1 Images
Western blot - Anti-AGPAT1 antibody (AB67018)
  • WB

Unknown

Western blot - Anti-AGPAT1 antibody (AB67018)

All lanes:

Western blot - Anti-AGPAT1 antibody (ab67018) at 1/500 dilution

Lane 1:

AGPAT1 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 32 kDa

Observed band size: 27 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human AGPAT1.

Q99943

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPAT1 also known as 1-acylglycerol-3-phosphate O-acyltransferase 1 performs an important role in lipid biosynthesis. It catalyzes the conversion of lysophosphatidic acid into phosphatidic acid a precursor for glycerolipid synthesis. The molecular mass of AGPAT1 is approximately 28 kDa. AGPAT1 expresses widely in human tissues with higher levels seen in adipose tissue liver and heart.
Biological function summary

AGPAT1 impacts lipid metabolism and energy homeostasis. It participates as a single enzyme and is not typically part of larger protein complexes. By facilitating the production of phosphatidic acid it influences the balance of lipid intermediates necessary for membrane and lipoprotein synthesis. This activity affects cellular processes like membrane dynamics and signal transduction.

Pathways

AGPAT1 plays an integral role in the glycerolipid metabolism pathway. It collaborates with enzymes like lipin-1 to regulate pathways that control lipid storage and release. Its action supports the phosphatidic acid pathway which connects to various cellular signaling cascades. AGPAT1 cooperates with other proteins that modulate lipid signals bringing about effects on cell proliferation and survival.

AGPAT1 associates notably with metabolic disorders and cardiovascular diseases. Dysregulation of AGPAT1 has implications for conditions like obesity and atherosclerosis as improper lipid metabolism can lead to excess lipid accumulation or energy imbalance. AGPAT1's interaction with proteins such as lipin-1 influences these disorders where lipid imbalance and chronic inflammation become important factors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
See full target information AGPAT1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 11: PubMed35052597

2021

Gallic Acid Improves Diabetic Steatosis by Downregulating MicroRNA-34a-5p through Targeting NFE2L2 Expression in High-Fat Diet-Fed db/db Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ang-Tse Lee,Mon-Yuan Yang,Yi-Ju Lee,Tzu-Wei Yang,Chi-Chih Wang,Chau-Jong Wang

Evidence-based complementary and alternative medicine : eCAM 2021:6658422 PubMed34035824

2021

Aqueous Mulberry Leaf Extract Ameliorates Alcoholic Liver Injury Associating with Upregulation of Ethanol Metabolism and Suppression of Hepatic Lipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ju Lee,Ming-Chang Tsai,Hui-Ting Lin,Chau-Jong Wang,Shao-Hsuan Kao

Cell death & disease 10:644 PubMed31501420

2019

CCT3 acts upstream of YAP and TFCP2 as a potential target and tumour biomarker in liver cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Liu,Xiao Zhang,Jiafei Lin,Yuxin Chen,Yongxia Qiao,Susu Guo,Yueyue Yang,Guoqing Zhu,Qiuhui Pan,Jiayi Wang,Fenyong Sun
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Product promise

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