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AB211435

Anti-AGPAT3 antibody

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(2 Publications)

Rabbit Polyclonal AGPAT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AGPAT3 aa 100-200.

View Alternative Names

LPAAT3, UNQ759/PRO1490, AGPAT3, 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, 1-acylglycerol-3-phosphate O-acyltransferase 3, Lysophosphatidic acid acyltransferase gamma, 1-AGP acyltransferase 3, 1-AGPAT 3, LPAAT-gamma

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT3 antibody (AB211435)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT3 antibody (AB211435)

Immunohistchemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling AGPAT3 with ab211435 at 10 μg/ml.

Western blot - Anti-AGPAT3 antibody (AB211435)
  • WB

Supplier Data

Western blot - Anti-AGPAT3 antibody (AB211435)

All lanes:

Western blot - Anti-AGPAT3 antibody (ab211435) at 1/500 dilution

Lane 1:

K562 cell lysate

Lane 2:

K562 cell lysate with Synthetic peptide from Human AGPAT3 (aa121-170)

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human AGPAT3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NRZ7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPAT3 also known as 1-acylglycerol-3-phosphate O-acyltransferase 3 is an enzyme that catalyzes the acylation of lysophosphatidic acid (LPA) to produce phosphatidic acid (PA). This enzyme performs its mechanical function in the endoplasmic reticulum and it plays a role in lipid metabolism. It has a mass of approximately 39 kDa. AGPAT3 expresses highly in tissues such as the heart skeletal muscle and adipose tissue suggesting its relevance in energy metabolism and fat storage.
Biological function summary

AGPAT3 acts as an important mediator in lipid biosynthesis specifically in the triacylglycerol and glycerophospholipid pathways. It contributes to the synthesis of phospholipids which are critical for cell membrane integrity and function. AGPAT3 does not function within a complex but acts as an individual enzyme facilitating the elongation of acyl chains in phospholipids. The presence of AGPAT3 in the endoplasmic reticulum marks it as integral in cellular lipid assembly and regulation.

Pathways

AGPAT3 is involved in the glycerolipid biosynthesis pathway and phospholipid biosynthesis. Its activity affects the production of phosphatidic acid a precursor for the synthesis of various lipids such as diacylglycerol and triacylglycerol. AGPAT3 works in concert with other AGPAT family members contributing to an intricate network that regulates lipid metabolism.

AGPAT3 associates with metabolic conditions like obesity and related cardiovascular diseases. The disruption of AGPAT3's function can lead to lipid accumulation and altered lipid signaling. AGPAT3's role in lipid metabolism closely connects it to other proteins such as lipoprotein lipase (LPL) influencing lipid clearance and energy balance. Understanding AGPAT3's function and regulation could provide insights into therapeutic approaches for these metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed : 21173190). Acts on LPA containing saturated or unsaturated fatty acids C16 : 0-C20 : 4 at the sn-1 position using C18 : 1, C20 : 4 or C18 : 2-CoA as the acyl donor (PubMed : 21173190). Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18 : 1 or C20 : 4-CoA (PubMed : 21173190). Has a preference for arachidonoyl-CoA as a donor (By similarity). Also has a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (By similarity).
See full target information AGPAT3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9550 PubMed39500869

2024

Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M T Herrera-Abreu,J Guan,U Khalid,J Ning,M R Costa,J Chan,Q Li,J-P Fortin,W R Wong,P Perampalam,A Biton,W Sandoval,J Vijay,M Hafner,R Cutts,G Wilson,J Frankum,T I Roumeliotis,J Alexander,O Hickman,R Brough,S Haider,J Choudhary,C J Lord,A Swain,C Metcalfe,N C Turner

Nucleic acids research 50:2872-2888 PubMed35150276

2022

Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Dörner,Lukas Badertscher,Bianka Horváth,Réka Hollandi,Csaba Molnár,Tobias Fuhrer,Roger Meier,Marie Sárazová,Jasmin van den Heuvel,Nicola Zamboni,Peter Horvath,Ulrike Kutay
View all publications
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Product promise

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