Rabbit Polyclonal AGPAT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AGPAT3 aa 100-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human AGPAT3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRZ7
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:21173190). Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor (PubMed:21173190). Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (PubMed:21173190). Has a preference for arachidonoyl-CoA as a donor (By similarity). Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (By similarity).
Alternative names
LPAAT3, UNQ759/PRO1490, AGPAT3, 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma, 1-acylglycerol-3-phosphate O-acyltransferase 3, Lysophosphatidic acid acyltransferase gamma, 1-AGP acyltransferase 3, 1-AGPAT 3, LPAAT-gamma
Recommended products
Rabbit Polyclonal AGPAT3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AGPAT3 aa 100-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human AGPAT3 aa 100-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NRZ7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AGPAT3 also known as 1-acylglycerol-3-phosphate O-acyltransferase 3 is an enzyme that catalyzes the acylation of lysophosphatidic acid (LPA) to produce phosphatidic acid (PA). This enzyme performs its mechanical function in the endoplasmic reticulum and it plays a role in lipid metabolism. It has a mass of approximately 39 kDa. AGPAT3 expresses highly in tissues such as the heart skeletal muscle and adipose tissue suggesting its relevance in energy metabolism and fat storage.
- Biological function summary
AGPAT3 acts as an important mediator in lipid biosynthesis specifically in the triacylglycerol and glycerophospholipid pathways. It contributes to the synthesis of phospholipids which are critical for cell membrane integrity and function. AGPAT3 does not function within a complex but acts as an individual enzyme facilitating the elongation of acyl chains in phospholipids. The presence of AGPAT3 in the endoplasmic reticulum marks it as integral in cellular lipid assembly and regulation.
- Pathways
AGPAT3 is involved in the glycerolipid biosynthesis pathway and phospholipid biosynthesis. Its activity affects the production of phosphatidic acid a precursor for the synthesis of various lipids such as diacylglycerol and triacylglycerol. AGPAT3 works in concert with other AGPAT family members contributing to an intricate network that regulates lipid metabolism.
- Associated diseases and disorders
AGPAT3 associates with metabolic conditions like obesity and related cardiovascular diseases. The disruption of AGPAT3's function can lead to lipid accumulation and altered lipid signaling. AGPAT3's role in lipid metabolism closely connects it to other proteins such as lipoprotein lipase (LPL) influencing lipid clearance and energy balance. Understanding AGPAT3's function and regulation could provide insights into therapeutic approaches for these metabolic diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-AGPAT3 antibody (ab211435)
All lanes: Western blot - Anti-AGPAT3 antibody (ab211435) at 1/500 dilution
Lane 1: K562 cell lysate
Lane 2: K562 cell lysate with Synthetic peptide from Human AGPAT3 (aa121-170)
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT3 antibody (ab211435)
Immunohistchemical analysis of formalin fixed, paraffin embedded Human kidney tissue labeling AGPAT3 with ab211435 at 10 μg/ml.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com