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AB135909

Anti-AGPAT4 antibody

4

(1 Review)

|

(1 Publication)

Rabbit Polyclonal AGPAT4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AGPAT4.

View Alternative Names

UNQ499/PRO1016, AGPAT4, 1-acyl-sn-glycerol-3-phosphate acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, Lysophosphatidic acid acyltransferase delta, 1-AGP acyltransferase 4, 1-AGPAT 4, LPAAT-delta

1 Images
Western blot - Anti-AGPAT4 antibody (AB135909)
  • WB

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Western blot - Anti-AGPAT4 antibody (AB135909)

All lanes:

Western blot - Anti-AGPAT4 antibody (ab135909) at 1/500 dilution

All lanes:

HeLa cell lysate at 30 µg

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AGPAT4. The exact immunogen used to generate this antibody is proprietary information.

Q9NRZ5

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPAT4 also known as 1-acylglycerol-3-phosphate O-acyltransferase 4 plays a mechanical role in glycerolipid biosynthesis. It catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by adding an acyl group. AGPAT4 has a mass of approximately 47 kDa. The highest expression of this enzyme occurs in tissues such as the liver kidney and brain indicating its importance in lipid metabolism in these areas.
Biological function summary

1-acylglycerol-3-phosphate O-acyltransferase enzymes like AGPAT4 are key in the synthesis of triacylglycerol and phospholipids components of cell membranes. AGPAT4 is not part of a larger complex but operates independently to perform its enzymatic function within the cell's lipid production pathways. This enzyme is essential for maintaining the balance of energy storage and membrane structure.

Pathways

AGPAT4 is a critical component in the glycerolipid metabolism pathway affecting lipid biosynthesis and energy homeostasis. It links with proteins such as AGPAT1 sharing overlapping roles in the production of phosphatidic acid which serves as a precursor for other lipids. AGPAT4 also engages in the phosphatidylinositol signaling pathway influencing downstream cellular functions and signaling processes.

AGPAT4 has connections to metabolic syndromes like obesity and liver disorders. Its altered function can disrupt normal lipid homeostasis leading to accumulation of lipids a characteristic of metabolic disease. Additionally AGPAT4 links with proteins like AGPAT2 in the context of congenital generalized lipodystrophy where defective lipid storage results in severe metabolic complications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Exhibits high acyl-CoA specificity for polyunsaturated fatty acyl-CoA, especially docosahexaenoyl-CoA (22 : 6-CoA, DHA-CoA) (By similarity).
See full target information AGPAT4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochimica et biophysica acta. Molecular and cell 1863:700-711 PubMed29627383

2018

Lpaatδ/Agpat4 deficiency impairs maximal force contractility in soleus and alters fibre type in extensor digitorum longus muscle.

Applications

WB

Species

Unspecified reactive species

Ryan M Bradley,Darin Bloemberg,Juan J Aristizabal Henao,Ashkan Hashemi,Andrew S Mitchell,Val A Fajardo,Catherine Bellissimo,Emily B Mardian,Eric Bombardier,Marie-France Paré,Katherine A Moes,Ken D Stark,A Russell Tupling,Joe Quadrilatero,Robin E Duncan
View all publications
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