Rabbit Polyclonal AGPAT5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGPAT5 aa 1-250.
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- Journal of animal science 91:4692-7042013Transcriptome changes favoring intramuscular fat deposition in the longissimus muscle following castration of bulls.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AGPAT5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUQ2
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:21173190). Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor (PubMed:21173190). Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA (PubMed:21173190). Activity toward lysophosphatidylglycerol not detectable (PubMed:21173190).
Alternative names
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-acylglycerol-3-phosphate O-acyltransferase 5, Lysophosphatidic acid acyltransferase epsilon, 1-AGP acyltransferase 5, 1-AGPAT 5, LPAAT-epsilon, AGPAT5
Recommended products
Rabbit Polyclonal AGPAT5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGPAT5 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AGPAT5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUQ2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AGPAT5 also known as 1-acylglycerol-3-phosphate O-acyltransferase 5 is an enzyme involved in lipid metabolism. It has a mass of approximately 47 kDa. AGPAT5 catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by adding an acyl group. This enzyme is expressed in various tissues with higher levels in adipose tissue and liver. Despite its wide expression its specific regulation mechanisms remain under investigation.
- Biological function summary
AGPAT5 plays a role in the biosynthesis of phospholipids and triglycerides. It is not commonly part of a large protein complex but it contributes significantly to the synthesis of important lipid molecules. These molecules are important for cell membrane formation and energy storage. AGPAT5 activity influences cellular lipid composition impacting membrane fluidity and cell signaling.
- Pathways
The enzyme AGPAT5 functions within the glycerolipid metabolism pathway. It interacts directly with intermediates like lysophosphatidic acid and phosphatidic acid which are key molecules in this pathway. The enzyme also links to the phosphatidic acid biosynthesis pathway sharing functional similarities with other AGPAT family members such as AGPAT1. These interactions highlight its role in maintaining cellular lipid balance.
- Associated diseases and disorders
AGPAT5 has associations with metabolic conditions like obesity and type 2 diabetes. Changes in lipid metabolism mediated by AGPAT5 can lead to altered adipose tissue function. Through its effects on lipid pathways AGPAT5 has indirect connections with other metabolic dysregulation proteins such as hormone-sensitive lipase (HSL). Further research into AGPAT5 could provide insights into therapeutic targets for these metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-AGPAT5 antibody (ab127749)
All lanes: Western blot - Anti-AGPAT5 antibody (ab127749) at 1/500 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 42 kDa
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