Rabbit Polyclonal AGPAT5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGPAT5 aa 1-250.
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- Journal of animal science 91:4692-7042013Transcriptome changes favoring intramuscular fat deposition in the longissimus muscle following castration of bulls.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AGPAT5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUQ2
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:21173190). Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor (PubMed:21173190). Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA (PubMed:21173190). Activity toward lysophosphatidylglycerol not detectable (PubMed:21173190).
Alternative names
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-acylglycerol-3-phosphate O-acyltransferase 5, Lysophosphatidic acid acyltransferase epsilon, 1-AGP acyltransferase 5, 1-AGPAT 5, LPAAT-epsilon, AGPAT5
Recommended products
Rabbit Polyclonal AGPAT5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGPAT5 aa 1-250.
Alternative names
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-acylglycerol-3-phosphate O-acyltransferase 5, Lysophosphatidic acid acyltransferase epsilon, 1-AGP acyltransferase 5, 1-AGPAT 5, LPAAT-epsilon, AGPAT5
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AGPAT5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUQ2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AGPAT5 also known as 1-acylglycerol-3-phosphate O-acyltransferase 5 is an enzyme involved in lipid metabolism. It has a mass of approximately 47 kDa. AGPAT5 catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by adding an acyl group. This enzyme is expressed in various tissues with higher levels in adipose tissue and liver. Despite its wide expression its specific regulation mechanisms remain under investigation.
- Biological function summary
AGPAT5 plays a role in the biosynthesis of phospholipids and triglycerides. It is not commonly part of a large protein complex but it contributes significantly to the synthesis of important lipid molecules. These molecules are important for cell membrane formation and energy storage. AGPAT5 activity influences cellular lipid composition impacting membrane fluidity and cell signaling.
- Pathways
The enzyme AGPAT5 functions within the glycerolipid metabolism pathway. It interacts directly with intermediates like lysophosphatidic acid and phosphatidic acid which are key molecules in this pathway. The enzyme also links to the phosphatidic acid biosynthesis pathway sharing functional similarities with other AGPAT family members such as AGPAT1. These interactions highlight its role in maintaining cellular lipid balance.
- Associated diseases and disorders
AGPAT5 has associations with metabolic conditions like obesity and type 2 diabetes. Changes in lipid metabolism mediated by AGPAT5 can lead to altered adipose tissue function. Through its effects on lipid pathways AGPAT5 has indirect connections with other metabolic dysregulation proteins such as hormone-sensitive lipase (HSL). Further research into AGPAT5 could provide insights into therapeutic targets for these metabolic disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-AGPAT5 antibody (ab127749)
All lanes: Western blot - Anti-AGPAT5 antibody (ab127749) at 1/500 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 42 kDa
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