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AB236541

Anti-AGPAT5 antibody

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(1 Publication)

Rabbit Polyclonal AGPAT5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGPAT5 aa 150-350.

View Alternative Names

1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon, 1-acylglycerol-3-phosphate O-acyltransferase 5, Lysophosphatidic acid acyltransferase epsilon, 1-AGP acyltransferase 5, 1-AGPAT 5, LPAAT-epsilon, AGPAT5

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT5 antibody (AB236541)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT5 antibody (AB236541)

Paraffin-embedded human pancreas tissue stained for AGPAT5 using ab236541 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT5 antibody (AB236541)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPAT5 antibody (AB236541)

Paraffin-embedded human endometrial cancer tissue stained for AGPAT5 using ab236541 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AGPAT5 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

Q9NUQ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPAT5 also known as 1-acylglycerol-3-phosphate O-acyltransferase 5 is an enzyme involved in lipid metabolism. It has a mass of approximately 47 kDa. AGPAT5 catalyzes the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA) by adding an acyl group. This enzyme is expressed in various tissues with higher levels in adipose tissue and liver. Despite its wide expression its specific regulation mechanisms remain under investigation.
Biological function summary

AGPAT5 plays a role in the biosynthesis of phospholipids and triglycerides. It is not commonly part of a large protein complex but it contributes significantly to the synthesis of important lipid molecules. These molecules are important for cell membrane formation and energy storage. AGPAT5 activity influences cellular lipid composition impacting membrane fluidity and cell signaling.

Pathways

The enzyme AGPAT5 functions within the glycerolipid metabolism pathway. It interacts directly with intermediates like lysophosphatidic acid and phosphatidic acid which are key molecules in this pathway. The enzyme also links to the phosphatidic acid biosynthesis pathway sharing functional similarities with other AGPAT family members such as AGPAT1. These interactions highlight its role in maintaining cellular lipid balance.

AGPAT5 has associations with metabolic conditions like obesity and type 2 diabetes. Changes in lipid metabolism mediated by AGPAT5 can lead to altered adipose tissue function. Through its effects on lipid pathways AGPAT5 has indirect connections with other metabolic dysregulation proteins such as hormone-sensitive lipase (HSL). Further research into AGPAT5 could provide insights into therapeutic targets for these metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed : 21173190). Acts on LPA containing saturated or unsaturated fatty acids C15 : 0-C20 : 4 at the sn-1 position using C18 : 1-CoA as the acyl donor (PubMed : 21173190). Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA (PubMed : 21173190). Activity toward lysophosphatidylglycerol not detectable (PubMed : 21173190).
See full target information AGPAT5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatric research 97:1209-1219 PubMed39103629

2024

Slow rewarming after hypothermia does not ameliorate white matter injury after hypoxia-ischemia in near-term fetal sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Alice McDouall,Kelly Q Zhou,Anthony Davies,Guido Wassink,Timothy L M Jones,Laura Bennet,Alistair J Gunn,Joanne O Davidson
View all publications
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Product promise

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