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AB242087

Anti-AGPAT7 antibody

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(1 Publication)

Rabbit Polyclonal AGPAT7 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LPCAT4 aa 450 to C-terminus.

View Alternative Names

AGPAT7, AYTL3, LPEAT2, LPCAT4, Lysophospholipid acyltransferase LPCAT4, 1-acylglycerol-3-phosphate O-acyltransferase 7, 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphoserine O-acyltransferase, 1-alkenylglycerophosphoethanolamine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acyltransferase-like 3, Lysophosphatidylcholine acyltransferase 4, Lysophosphatidylethanolamine acyltransferase 2, Plasmalogen synthase, 1-AGP acyltransferase 7, 1-AGPAT 7

1 Images
Immunoprecipitation - Anti-AGPAT7 antibody (AB242087)
  • IP

Supplier Data

Immunoprecipitation - Anti-AGPAT7 antibody (AB242087)

AGPAT7 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242087 at 8 μl/reaction. Western blot was performed from the immunoprecipitate using ab242087 at 1/400 dilution.

Lane 1 : ab242087 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-AGPAT7 antibody (ab242087)

Predicted band size: 57 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human LPCAT4 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q643R3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to AGPAT7 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPAT7 also known as 1-acylglycerol-3-phosphate O-acyltransferase 7 is an enzyme that plays a role in lipid biosynthesis by converting lysophosphatidic acid (LPA) into phosphatidic acid (PA). This enzyme has a molecular mass of approximately 37 kilodaltons (kDa). In terms of its expression profile AGPAT7 can be found in various tissues including the liver and adipose tissue suggesting its involvement in lipid metabolism across different biological systems.
Biological function summary

This protein is associated with the membrane of the endoplasmic reticulum where it is responsible for the acylation step in the glycerolipid biosynthesis pathway. AGPAT7 does not form part of a larger complex but acts in concert with other enzymes involved in lipid production. Its ability to catalyze the acylation process marks its significance in synthesizing a wide range of lipid molecules impacting membrane composition and signaling pathways.

Pathways

AGPAT7 is directly involved in the glycerophospholipid metabolism pathway which is a component of the broader lipid metabolic process. It also contributes to the phosphatidic acid biosynthesis pathway linking it to other key players such as AGPAT2 and LPAAT which function together in regulating lipid intermediates and ensuring the steady production of phosphatidic acid a precursor for triglycerides and phospholipids.

AGPAT7's role in lipid biosynthesis has implications for metabolic diseases such as obesity and metabolic syndrome. Alterations in AGPAT7 expression might disrupt lipid homeostasis potentially leading to these conditions. Moreover its connection to AGPAT2 known for its involvement in congenital generalized lipodystrophy highlights the enzyme's importance in maintaining lipid balance. Understanding AGPAT7's function in these contexts might offer new insights into therapeutic approaches for lipid-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Displays acyl-CoA-dependent lysophospholipid acyltransferase activity with a subset of lysophospholipids as substrates; converts lysophosphatidylethanolamine to phosphatidylethanolamine, lysophosphatidylcholine to phosphatidycholine, 1-alkenyl-lysophatidylethanolamine to 1-alkenyl-phosphatidylethanolamine, lysophosphatidylglycerol and alkyl-lysophosphatidylcholine to phosphatidylglycerol and alkyl-phosphatidylcholine, respectively. In contrast, has no lysophosphatidylinositol, glycerol-3-phosphate, diacylglycerol or lysophosphatidic acid acyltransferase activity. Prefers long chain acyl-CoAs (C16, C18) as acyl donors.
See full target information LPCAT4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:3 PubMed34815755

2021

Hirudin, a thrombin inhibitor, attenuates TGF-β-induced fibrosis in renal proximal tubular epithelial cells by inhibition of protease-activated receptor 1 expression via S1P/S1PR2/S1PR3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Lin,Chunli Long,Zhengang Wang,Ronghui Wang,Wei Shi,Jiwei Qiu,Junlin Mo,Yongxiang Xie
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Product promise

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