AB236621

Anti-AGPS antibody

Name: Anti-AGPS antibody (ab236621) | Abcam
Brand: Abcam Limited
SKU: AB236621

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(6 Publications)

Rabbit Polyclonal AGPS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGPS aa 250-400.

View Alternative Names

AAG5, AGPS, Alkyl-DHAP synthase, Aging-associated gene 5 protein, Alkylglycerone-phosphate synthase

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody (AB236621)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody (AB236621)

Paraffin-embedded human pancreatic cancer tissue stained for AGPS with ab236621 at a 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-AGPS antibody (AB236621)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AGPS antibody (AB236621)

NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for AGPS using ab236621 at a dilution of 1/166 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human AGPS aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

O00116

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPS also known as alkylglycerone phosphate synthase represents an important enzyme in lipid metabolism with a molecular mass of approximately 73 kDa. It catalyzes the formation of alkylglycerone phosphate from dihydroxyacetone phosphate and fatty alcohols a reaction that occurs in peroxisomes. AGPS is expressed in various tissues with high levels observed in the brain heart and reproductive organs. Its expression is important for proper plasmalogen synthesis an essential lipid component of cell membranes.

Biological function summary

AGPS functions as an important component in the synthesis of ether lipids specifically plasmalogens which play a role in membrane structure and signaling. Plasmalogens are phospholipids that are part of cellular membranes and influence membrane fluidity and dynamics. AGPS operates as a sole enzyme in the initial step of de novo plasmalogen biosynthesis engaging with other enzymes to complete this lipid complex. Its activity ensures the proper formation of plasmalogens which are particularly abundant in the heart and nervous tissues.

Pathways

AGPS plays a central role in the ether lipid biosynthesis pathway and interacts with proteins involved in lipid metabolism. Plasmalogen biosynthesis is a critical pathway where AGPS catalyzes the first step acting as a precursor in the synthesis of several ether lipids. Within the biosynthetic pathway AGPS works alongside enzymes such as fatty acyl-CoA reductase highlighting its collaboration with other proteins to regulate lipid homeostasis in cellular membranes.

Defects in AGPS can relate to conditions such as rhizomelic chondrodysplasia punctata (RCDP) and Zellweger spectrum disorders. RCDP presents severe skeletal malformations among other symptoms while Zellweger disorders involve multiple organ dysfunctions due to peroxisomal biogenesis issues. Mutations in AGPS often result in developmental delays and neurological impairment. Additionally AGPS dysfunction affects proteins like PEX7 which is important for targeting peroxisomal enzymes further exacerbating the disease phenotypes associated with these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl-dihydroxyacetonephosphate (alkyl-DHAP), in the pathway of ether lipid biosynthesis.

See full target information AGPS

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Biological procedures online 27:33 PubMed40836218

2025

Refining Flow Cytometry-based Sorting of Plasma-derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Reverberi,Maria Chiara Ciferri,Nicole Rosenwasser,Alessandro Catino,Giuseppina Poppa,Ilaria Giusti,Vincenza Dolo,Rodolfo Quarto,Sara Santamaria,Monica Colombo,Simona Coco,Roberta Tasso

Nature 645:1011-1019 PubMed40804518

2025

Multiple oestradiol functions inhibit ferroptosis and acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wulf Tonnus,Francesca Maremonti,Shubhangi Gavali,Marlena Nastassja Schlecht,Florian Gembardt,Alexia Belavgeni,Nadja Leinung,Karolin Flade,Natalie Bethe,Sofia Traikov,Anne Haag,Danny Schilling,Sider Penkov,Melodie Mallais,Christine Gaillet,Claudia Meyer,Melika Katebi,Anushka Ray,Louisa M S Gerhardt,Anne Brucker,Jorunn Naila Becker,Mirela Tmava,Lisa Schlicker,Almut Schulze,Nina Himmerkus,Andrej Shevchenko,Mirko Peitzsch,Uladzimir Barayeu,Sonia Nasi,Juliane Putz,Kenneth S Korach,Joel Neugarten,Ladan Golestaneh,Christian Hugo,Jan Ulrich Becker,Joel M Weinberg,Svenja Lorenz,Bettina Proneth,Marcus Conrad,Eckhard Wolf,Bernd Plietker,Raphaël Rodriguez,Derek A Pratt,Tobias P Dick,Maria Fedorova,Stefan R Bornstein,Andreas Linkermann

Science advances 11:eadu9104 PubMed40601736

2025

FAF2 is a bifunctional regulator of peroxisomal homeostasis and saturated lipid responses.

Applications

Unspecified application

Species

Unspecified reactive species

Choah Kim,Katlyn R Gabriel,Dylan Boone,Matthew R Brown,Katherine Oppenheimer,Maria Kost-Alimova,Juan Lorenzo B Pablo,Anna Greka

Experimental & molecular medicine 56:441-452 PubMed38383581

2024

Helicobacter pylori CagA-mediated ether lipid biosynthesis promotes ferroptosis susceptibility in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmei Peng,Xuetao Lei,Qingbin Yang,Guofan Zhang,Sixiao He,Minghao Wang,Ruoyu Ling,Boyang Zheng,Jiayong He,Xinhua Chen,Fengping Li,Qiming Zhou,Liying Zhao,Gengtai Ye,Guoxin Li

Respiratory research 24:301 PubMed38041059

2023

CSE triggers ferroptosis via SIRT4-mediated GNPAT deacetylation in the pathogenesis of COPD.

Applications

Unspecified application

Species

Unspecified reactive species

Congping Li,Fei Chen,Liangfen Lin,Jiwei Li,Yamei Zheng,Qingyun Chen

Cell chemical biology 29:157-170.e6 PubMed34813762

2021

PALP: A rapid imaging technique for stratifying ferroptosis sensitivity in normal and tumor tissues in situ.

Applications

Unspecified application

Species

Unspecified reactive species

Fengxiang Wang,Emily T Graham,Nathchar Naowarojna,Zhennan Shi,Yuqi Wang,Guanglei Xie,Lili Zhou,Wendy Salmon,Jie-Min Jia,Xi Wang,Yuwei Huang,Stuart L Schreiber,Yilong Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AGPS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody (AB236621)

Immunocytochemistry/ Immunofluorescence - Anti-AGPS antibody (AB236621)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise