JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB184186

Anti-AGPS antibody [EPR13120] - N-terminal

4

(2 Reviews)

|

(3 Publications)

Rabbit Recombinant Monoclonal AGPS antibody. N-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

AAG5, AGPS, Alkyl-DHAP synthase, Aging-associated gene 5 protein, Alkylglycerone-phosphate synthase

5 Images
Flow Cytometry (Intracellular) - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling AGPS with ab184186 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling AGPS with ab184186 at a 1/100 dilution.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)
  • WB

Supplier Data

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)

All lanes:

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

SW480 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

true

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)
  • WB

Supplier Data

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)

All lanes:

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186) at 1/2000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

true

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)
  • WB

Supplier Data

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (AB184186)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling AGPS with ab184186 at a 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 diluiton.

All lanes:

Western blot - Anti-AGPS antibody [EPR13120] - N-terminal (ab184186)

Predicted band size: 73 kDa

false

  • Carrier free

    Anti-AGPS antibody [EPR13120] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13120

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB236621

Anti-AGPS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGPS antibody (AB236621)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75060

Anti-GNPAT/DAPAT antibody

Western blot - Anti-GNPAT/DAPAT antibody (AB75060)

  • 4
  • 1
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 18
Primary Antibodies

AB13840

Anti-DDX4 / MVH antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX4 / MVH antibody (AB13840)

  • 5
  • 22
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGPS also known as alkylglycerone phosphate synthase represents an important enzyme in lipid metabolism with a molecular mass of approximately 73 kDa. It catalyzes the formation of alkylglycerone phosphate from dihydroxyacetone phosphate and fatty alcohols a reaction that occurs in peroxisomes. AGPS is expressed in various tissues with high levels observed in the brain heart and reproductive organs. Its expression is important for proper plasmalogen synthesis an essential lipid component of cell membranes.
Biological function summary

AGPS functions as an important component in the synthesis of ether lipids specifically plasmalogens which play a role in membrane structure and signaling. Plasmalogens are phospholipids that are part of cellular membranes and influence membrane fluidity and dynamics. AGPS operates as a sole enzyme in the initial step of de novo plasmalogen biosynthesis engaging with other enzymes to complete this lipid complex. Its activity ensures the proper formation of plasmalogens which are particularly abundant in the heart and nervous tissues.

Pathways

AGPS plays a central role in the ether lipid biosynthesis pathway and interacts with proteins involved in lipid metabolism. Plasmalogen biosynthesis is a critical pathway where AGPS catalyzes the first step acting as a precursor in the synthesis of several ether lipids. Within the biosynthetic pathway AGPS works alongside enzymes such as fatty acyl-CoA reductase highlighting its collaboration with other proteins to regulate lipid homeostasis in cellular membranes.

Defects in AGPS can relate to conditions such as rhizomelic chondrodysplasia punctata (RCDP) and Zellweger spectrum disorders. RCDP presents severe skeletal malformations among other symptoms while Zellweger disorders involve multiple organ dysfunctions due to peroxisomal biogenesis issues. Mutations in AGPS often result in developmental delays and neurological impairment. Additionally AGPS dysfunction affects proteins like PEX7 which is important for targeting peroxisomal enzymes further exacerbating the disease phenotypes associated with these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl-dihydroxyacetonephosphate (alkyl-DHAP), in the pathway of ether lipid biosynthesis.
See full target information AGPS
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu9104 PubMed40601736

2025

FAF2 is a bifunctional regulator of peroxisomal homeostasis and saturated lipid responses.

Applications

Unspecified application

Species

Unspecified reactive species

Choah Kim,Katlyn R Gabriel,Dylan Boone,Matthew R Brown,Katherine Oppenheimer,Maria Kost-Alimova,Juan Lorenzo B Pablo,Anna Greka

The FEBS journal 291:4714-4731 PubMed39206622

2024

PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglan Hao,Guiqin Han,Xin Liang,Yongtong Ruan,Chen Huang,Naer Sa,Hang Hu,Bixi Hu,Zhongqi Li,Kai Zhang,Ping Gao,Xiaoming Dong

Journal of biomedical research 38:24-36 PubMed38062668

2023

Germ cell-specific deletion of reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yejin Yao,Baolu Shi,Xiangzheng Zhang,Xin Wang,Shuangyue Li,Ying Yao,Yueshuai Guo,Dingdong Chen,Bing Wang,Yan Yuan,Jiahao Sha,Xuejiang Guo
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com