Rabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Recombinant fragment - Human, Human, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--immunocytochemistry-immunofluorescence-img48454.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)")
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img24951.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)")
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img9283.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)")
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img50963.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)")
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img9577.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Recombinant fragment - Human, Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).
Additional Targets
AGR3
Alternative names
AG2, UNQ515/PRO1030, AGR2, Anterior gradient protein 2 homolog, AG-2, hAG-2, HPC8, Secreted cement gland protein XAG-2 homolog
Recommended products
Rabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Recombinant fragment - Human, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20164-222
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251553 is the carrier-free version of Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AGR2 (anterior gradient protein 2) and AGR3 (anterior gradient protein 3) are small proteins with roles in cellular homeostasis. AGR2 has a mass of approximately 20 kDa while AGR3 is around 18 kDa. Both proteins localize in the endoplasmic reticulum (ER) and are expressed notably in epithelial tissues like the gastrointestinal tract and respiratory epithelium. AGR2 also known as PDIA17 functions as a protein disulfide isomerase which assists in protein folding. AGR3 shares structural similarities with AGR2 suggesting overlapping functions.
- Biological function summary
AGR2 and AGR3 influence processes such as cell growth and repair. They are necessary for maintaining the proper folding of secreted and membrane proteins. AGR2 interacts with mucin proteins and this complex formation is important for mucin production which supports protective mucus barriers. AGR3's specific biological partners are less well-defined but it complements AGR2's functions in similar tissue contexts suggesting it might share similar interactions.
- Pathways
AGR2 and AGR3 engage in pathways that link ER function to broader cellular activities. One important pathway involves the unfolded protein response (UPR) where AGR2 helps mitigate ER stress by ensuring proper protein assembly and folding. Additionally AGR2 participates in the ERK signaling pathway impacting cell proliferation and differentiation. Proteins such as ATF6 and GRP78 interact with AGR2 in these pathways indicating its role in stress response and growth signaling networks.
- Associated diseases and disorders
AGR2 and AGR3 gain attention in relation to cancer and cystic fibrosis. In cancer especially breast and prostate AGR2 overexpression correlates with tumor progression and metastasis. It interacts with proteins like MUC1 and HER2 facilitating oncogenic pathways. AGR3 while less studied shows similar trends suggesting potential involvement. In cystic fibrosis AGR2 associates with compromised mucin production and secretion causing exacerbated lung conditions. CXCL8 a mucin-related protein is seen alongside AGR2 activity in these inflammatory scenarios.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--immunocytochemistry-immunofluorescence-img48454.jpg "Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cell line.Negative control: HeLa (PMID: 16551856). The nuclear counterstain is DAPI (blue). Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img24951.jpg "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds; Lane 2: 3 seconds.
All lanes: Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071) at 1/10000 dilution
Lane 1: Human AGR2 His-tagged recombinant protein (aa21-175) at 0.01 µg
Lane 2: Human AGR3 GST-tagged recombinant protein (aa21-166) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 19 kDa, 20 kDa
Observed band size: 17 kDa, 42 kDa
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img9283.jpg "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
According to the literature AGR2 is not expressed in HeLa (PMID: 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.
All lanes: Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071) at 1/1000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2: HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3: PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 19 kDa, 20 kDa
Observed band size: 17 kDa
Exposure time: 1min
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img50963.jpg "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071) at 1/1000 dilution
Lane 1: Mouse stomach tissue lysate at 10 µg
Lane 2: Rat stomach tissue lysate at 10 µg
Lane 3: Human lung tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 19 kDa, 20 kDa
Observed band size: 17 kDa
Exposure time: 3min
![Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--western-blot-img9577.jpg "Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071) at 1/2000 dilution
All lanes: Human stomach lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 19 kDa, 20 kDa
Observed band size: 17 kDa, 18 kDa
Exposure time: 1s
![Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--flow-cytometry-intracellular-img26731.jpg "Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--flow-cytometry-intracellular-img25131.jpg "Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)
This data was developed using Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with Anti-AGR2 + AGR3 antibody [EPR20164-222] ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--flow-cytometry-intracellular-img26731.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
![Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553), expandable thumbnail](https://content.abcam.com/products/images/agr2-agr3-antibody-epr20164-222-bsa-and-azide-free-ab251553--flow-cytometry-intracellular-img25131.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (ab251553)")
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