AB251553

Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Anterior Gradient 2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Recombinant fragment - Human, Human, Rat samples. Cited in 1 publication.

View Alternative Names

AG2, UNQ515/PRO1030, AGR2, Anterior gradient protein 2 homolog, AG-2, hAG-2, HPC8, Secreted cement gland protein XAG-2 homolog, BCMP11, PDIA18, UNQ642/PRO1272, AGR3, Anterior gradient protein 3, AG-3, AG3, hAG-3, Anterior gradient 3 homolog, Breast cancer membrane protein 11

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF7 cell line.

Negative control : HeLa (PMID : 16551856).

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AGR2 + AGR3 with ab216071 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AGR2 + AGR3 with ab216071 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (<a href='/en-us/products/primary-antibodies/agr2-agr3-antibody-epr20164-222-ab216071'>ab216071</a>) at 1/1000 dilution

Lane 1:

Mouse stomach tissue lysate at 10 µg

Lane 2:

Rat stomach tissue lysate at 10 µg

Lane 3:

Human lung tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (<a href='/en-us/products/primary-antibodies/agr2-agr3-antibody-epr20164-222-ab216071'>ab216071</a>) at 1/2000 dilution

All lanes:

Human stomach lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa,18 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

According to the literature AGR2 is not expressed in HeLa (PMID : 16551856). Currently no published data is available regarding the expression of AGR3 in HeLa cells.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (<a href='/en-us/products/primary-antibodies/agr2-agr3-antibody-epr20164-222-ab216071'>ab216071</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

This data was developed using ab216071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 seconds; Lane 2 : 3 seconds.

All lanes:

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] (<a href='/en-us/products/primary-antibodies/agr2-agr3-antibody-epr20164-222-ab216071'>ab216071</a>) at 1/10000 dilution

Lane 1:

Human AGR2 His-tagged recombinant protein (aa21-175) at 0.01 µg

Lane 2:

Human AGR3 GST-tagged recombinant protein (aa21-166) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 19 kDa,20 kDa

Observed band size: 17 kDa,42 kDa

false

Unconjugated

Anti-AGR2 + AGR3 antibody [EPR20164-222]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20164-222

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab251553 is the carrier-free version of ab216071.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGR2 (anterior gradient protein 2) and AGR3 (anterior gradient protein 3) are small proteins with roles in cellular homeostasis. AGR2 has a mass of approximately 20 kDa while AGR3 is around 18 kDa. Both proteins localize in the endoplasmic reticulum (ER) and are expressed notably in epithelial tissues like the gastrointestinal tract and respiratory epithelium. AGR2 also known as PDIA17 functions as a protein disulfide isomerase which assists in protein folding. AGR3 shares structural similarities with AGR2 suggesting overlapping functions.

Biological function summary

AGR2 and AGR3 influence processes such as cell growth and repair. They are necessary for maintaining the proper folding of secreted and membrane proteins. AGR2 interacts with mucin proteins and this complex formation is important for mucin production which supports protective mucus barriers. AGR3's specific biological partners are less well-defined but it complements AGR2's functions in similar tissue contexts suggesting it might share similar interactions.

Pathways

AGR2 and AGR3 engage in pathways that link ER function to broader cellular activities. One important pathway involves the unfolded protein response (UPR) where AGR2 helps mitigate ER stress by ensuring proper protein assembly and folding. Additionally AGR2 participates in the ERK signaling pathway impacting cell proliferation and differentiation. Proteins such as ATF6 and GRP78 interact with AGR2 in these pathways indicating its role in stress response and growth signaling networks.

AGR2 and AGR3 gain attention in relation to cancer and cystic fibrosis. In cancer especially breast and prostate AGR2 overexpression correlates with tumor progression and metastasis. It interacts with proteins like MUC1 and HER2 facilitating oncogenic pathways. AGR3 while less studied shows similar trends suggesting potential involvement. In cystic fibrosis AGR2 associates with compromised mucin production and secretion causing exacerbated lung conditions. CXCL8 a mucin-related protein is seen alongside AGR2 activity in these inflammatory scenarios.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed : 23274113).

See full target information AGR2

Additional targets

AGR3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 39:1479-1496.e18 PubMed34653364

2021

Signatures of plasticity, metastasis, and immunosuppression in an atlas of human small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph M Chan,Álvaro Quintanal-Villalonga,Vianne Ran Gao,Yubin Xie,Viola Allaj,Ojasvi Chaudhary,Ignas Masilionis,Jacklynn Egger,Andrew Chow,Thomas Walle,Marissa Mattar,Dig V K Yarlagadda,James L Wang,Fathema Uddin,Michael Offin,Metamia Ciampricotti,Besnik Qeriqi,Amber Bahr,Elisa de Stanchina,Umesh K Bhanot,W Victoria Lai,Matthew J Bott,David R Jones,Arvin Ruiz,Marina K Baine,Yanyun Li,Natasha Rekhtman,John T Poirier,Tal Nawy,Triparna Sen,Linas Mazutis,Travis J Hollmann,Dana Pe'er,Charles M Rudin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Flow Cytometry (Intracellular) - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Western blot - Anti-AGR2 + AGR3 antibody [EPR20164-222] - BSA and Azide free (AB251553)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise