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Rabbit Recombinant Multiclonal AGRIN antibody. Suitable for WB, IHC-Fr, IP and reacts with Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (AB324022), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (AB324022), expandable thumbnail
  • Western blot - Anti-Agrin antibody [RM2082] (AB324022), expandable thumbnail
  • Western blot - Anti-Agrin antibody [RM2082] (AB324022), expandable thumbnail
  • Immunoprecipitation - Anti-Agrin antibody [RM2082] (AB324022), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIPIHC-PICC/IFFlow Cyt
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Isoform 1. Heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases. Isoform 2 and isoform 3: these isoforms lacking the 'z' insert (z0) are muscle-specific, have no AChR clustering ability and may be involved in nervous system endothelial cell differentiation. Agrin N-terminal 110 kDa subunit. Involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling (By similarity). Agrin C-terminal 22 kDa fragment. This released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.

Alternative names

Agrin

Rabbit Recombinant Multiclonal AGRIN antibody. Suitable for WB, IHC-Fr, IP and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM2082
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Immunohistochemistry (Frozen sections) staining of Rat lung (fresh frozen) using rabbit Anti-Agrin antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung (fresh frozen) tissue labeling Agrin with ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining in endothelial cells on rat lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab324022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Immunohistochemistry (Frozen sections) staining of Rat kidney (fresh frozen) using rabbit Anti-Agrin antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh frozen) tissue labeling Agrin with ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining in glomeruli of rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab324022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Western blot - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Western blot - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Western blot staining using rabbit Anti-Agrin antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18230682, 17586728).

    The identity of the band at approximately 50 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Agrin antibody [RM2082] (ab324022) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 80 µg

    Lane 2: Mouse ovary tissue lysate at 80 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 22 kDa, 90 kDa, 250-300 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Western blot - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Western blot staining using rabbit Anti-Agrin antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: RAW 264.7, A20.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18230682, 17586728).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Agrin antibody [RM2082] (ab324022) at 1/1000 dilution

    Lane 1: mIMCD3 (mouse inner medullary collecting duct epithelial cell) whole cell lysate at 80 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 80 µg

    Lane 3: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 80 µg

    Lane 4: A20 (mouse reticulum sarcoma B lymphocyte) whole cell lysate at 80 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 22 kDa, 90 kDa, 250-300 kDa, 36 kDa

    Exposure time: 114s

  • Immunoprecipitation - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Immunoprecipitation - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin was immunoprecipitated from 0.35 mg ab324022 at 1/30 IP in Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab324022 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324022 at 1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-Agrin antibody [RM2082] (ab324022) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 10 µg

    Lane 2: ab324022 at 1/30 IP in Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324022 in Neuro-2a whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 250-300 kDa

    Exposure time: 180s

  • Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Immunohistochemistry (Frozen sections) staining of Mouse lung (fresh frozen) using rabbit Anti-Agrin antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lung (fresh frozen) tissue labeling Agrin with ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining in endothelial cells on mouse lung. The nuclear counterstain was DAPI (Blue). The section was incubated with ab324022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Immunohistochemistry (Frozen sections) staining of Mouse cerebrum (fresh frozen) using rabbit Anti-Agrin antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh frozen) tissue labeling Agrin with ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Panel A: merged staining of anti-AGRN (ab324022, green), anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, white) and anti-GFAP (Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732, magenta) on mouse cerebrum.

    Panel B: anti-AGRN stained in endothelial cells on mouse cerebrum.

    Panel C: anti-NeuN stained in neurons of mouse cerebrum.

    Panel D: anti-GFAP stained in astrocytes of mouse cerebrum.

    The section was incubated in two rounds of staining: in the order of ab324022 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, Alexa Fluor® 594 Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Agrin antibody [RM2082] (ab324022)

    Agrin Immunohistochemistry (Frozen sections) staining of Mouse kidney (fresh frozen) using rabbit Anti-Agrin antibody

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney (fresh frozen) tissue labeling Agrin with ab324022 at 1/50 (9.98 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining in glomeruli of mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab324022 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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Product protocols

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