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AB113481

Anti-AGRP antibody

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(4 Publications)

Rabbit Polyclonal AGRP antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human AGRP.

View Alternative Names

AGRT, ART, AGRP, Agouti-related protein

1 Images
Western blot - Anti-AGRP antibody (AB113481)
  • WB

Unknown

Western blot - Anti-AGRP antibody (AB113481)

All lanes:

Western blot - Anti-AGRP antibody (ab113481) at 1/500 dilution

Lane 1:

Human fetal brain lysate

Lane 2:

Human fetal testis lysate

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AGRP. The exact immunogen used to generate this antibody is proprietary information.

O00253

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Immunogen
Purification notes
ab113481 is purified by a peptide affinity column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGRP also known as Agouti-related protein is a neuropeptide with a molecular weight of approximately 13.5 kDa. It is predominantly expressed in the arcuate nucleus of the hypothalamus in the brain and some regions of the adrenal gland. AGRP serves as an antagonist to melanocortin receptors particularly the melanocortin 3 and 4 receptors (MC3R and MC4R) which are involved in energy homeostasis. By binding to these receptors AGRP plays a critical role in the regulation of appetite and energy balance by inhibiting the action of alpha-melanocyte-stimulating hormone.
Biological function summary

AGRP is involved in the regulation of feeding behavior and body weight. It does not function as part of a larger complex but operates effectively as an antagonist in its own right. When expressed AGRP influences energy expenditure and food intake contributing to how the body regulates hunger and satiety. Its activity helps to modulate signaling pathways that can increase the desire to eat when energy stores are depleted.

Pathways

AGRP plays a notable role in the melanocortin pathway and influences the hypothalamic signaling pathways related to energy balance. It interacts with melanocortin receptors as part of this system modulating signals that maintain energy homeostasis. AGRP is closely related to orexigenic pathways working alongside other proteins like neuropeptide Y (NPY) which further stimulate appetite and food intake.

AGRP has a strong connection to obesity and metabolic disorders. It is implicated in conditions where energy balance is disrupted leading to excessive weight gain. AGRP levels can be elevated in individuals with obesity suggesting a link between its upregulation and increased appetite. Additionally the protein interacts with leptin a hormone involved in energy expenditure and appetite suppression highlighting AGRP's role in metabolic conditions where leptin signaling may be impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signaling protein that functions as an antagonist of melanocyte-stimulating-hormone receptors MC3R and MC4R by precluding agonist-induced signaling, thereby inhibiting cAMP production within the hypothalamus and adrenal gland (PubMed : 10371151, PubMed : 11145747, PubMed : 9311920, PubMed : 9892020). Involved in the control of feeding behavior via the central melanocortin system (PubMed : 15927146, PubMed : 9311920). Has very low activity towards MC5R (PubMed : 9311920, PubMed : 9892020). Also promotes endocytosis of MC3R and MC4R in an arrestin-dependent mechanism (PubMed : 17041250).
See full target information AGRP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:34 PubMed38448811

2024

Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1.

Applications

Unspecified application

Species

Unspecified reactive species

Hengru Guo,Ying Xin,Saifei Wang,Xiaoning Zhang,Yanqi Ren,Bo Qiao,Hongjiang Li,Jing Wu,Xiao Hao,Lijun Xu,Yushan Yan,Haohao Zhang

Frontiers in endocrinology 13:932761 PubMed36387867

2022

Involvement of POMC neurons in LEAP2 regulation of food intake and body weight.

Applications

Unspecified application

Species

Unspecified reactive species

Guangpin Chu,Hualing Peng,Nana Yu,Yuejin Zhang,Xueling Lin,Yisheng Lu

Molecular metabolism 3:441-51 PubMed24944903

2014

Hypothalamic KLF4 mediates leptin's effects on food intake via AgRP.

Applications

IHC-P

Species

Rat

Monica Imbernon,Estrella Sanchez-Rebordelo,Rosalia Gallego,Marina Gandara,Pamela Lear,Miguel Lopez,Carlos Dieguez,Ruben Nogueiras

PloS one 8:e79708 PubMed24260287

2013

Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR.

Applications

WB

Species

Human

Rebecca Lopez,Arunkumar Arumugam,Riya Joseph,Kanika Monga,Thiyagarajan Boopalan,Pamela Agullo,Christina Gutierrez,Sushmita Nandy,Ramadevi Subramani,Jose Manuel de la Rosa,Rajkumar Lakshmanaswamy
View all publications
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Product promise

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