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AB228495

Anti-AGRP antibody

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(2 Publications)

Guinea pig Polyclonal Agrp antibody. Suitable for IHC-Fr and reacts with Rat, Sheep samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Agrp aa 50 to C-terminus.

View Alternative Names

AGRP_HUMAN, AGRT, ART, ASIP2, Agouti Related Protein Homolog, Agouti related neuropeptide, Agouti-related protein

2 Images
Immunohistochemistry (Frozen sections) - Anti-AGRP antibody (AB228495)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-AGRP antibody (AB228495)

4% paraformaldehyde-fixed, frozen section of rat brain tissue stained for AGRP using ab228495 at 1/1000 dilution in immunohistochemical analysis, followed by detection with Alexa Fluor® 568 Goat anti-Guinea pig at 1/500 dilution.

Immunohistochemistry (Frozen sections) - Anti-AGRP antibody (AB228495)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-AGRP antibody (AB228495)

4% paraformaldehyde-fixed, frozen section of sheep hypothalamus tissue stained for AGRP using ab228495 at 1/1000 dilution in immunohistochemical analysis, followed by detection with a biotinylated secondary antibody.

Key facts

Host species

Guinea pig

Clonality

Polyclonal

Carrier free

No

Reacts with

Rat, Sheep

Applications

IHC-Fr

applications

Immunogen

Synthetic Peptide within Mouse Agrp aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P56473

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000 - 1/2000", "IHCFr-species-notes": "<p>Permeabilization suggested: 0.1% Triton X-100 in blocking buffer.</p>" }, "Cow": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Sheep": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000 - 1/2000", "IHCFr-species-notes": "<p>Permeabilization suggested: 0.1% Triton X-100 in blocking buffer.</p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 50µL
Purity
Whole antiserum
Storage buffer
pH: 7 - 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGRP also known as Agouti-related protein is a neuropeptide with a molecular weight of approximately 13.5 kDa. It is predominantly expressed in the arcuate nucleus of the hypothalamus in the brain and some regions of the adrenal gland. AGRP serves as an antagonist to melanocortin receptors particularly the melanocortin 3 and 4 receptors (MC3R and MC4R) which are involved in energy homeostasis. By binding to these receptors AGRP plays a critical role in the regulation of appetite and energy balance by inhibiting the action of alpha-melanocyte-stimulating hormone.
Biological function summary

AGRP is involved in the regulation of feeding behavior and body weight. It does not function as part of a larger complex but operates effectively as an antagonist in its own right. When expressed AGRP influences energy expenditure and food intake contributing to how the body regulates hunger and satiety. Its activity helps to modulate signaling pathways that can increase the desire to eat when energy stores are depleted.

Pathways

AGRP plays a notable role in the melanocortin pathway and influences the hypothalamic signaling pathways related to energy balance. It interacts with melanocortin receptors as part of this system modulating signals that maintain energy homeostasis. AGRP is closely related to orexigenic pathways working alongside other proteins like neuropeptide Y (NPY) which further stimulate appetite and food intake.

AGRP has a strong connection to obesity and metabolic disorders. It is implicated in conditions where energy balance is disrupted leading to excessive weight gain. AGRP levels can be elevated in individuals with obesity suggesting a link between its upregulation and increased appetite. Additionally the protein interacts with leptin a hormone involved in energy expenditure and appetite suppression highlighting AGRP's role in metabolic conditions where leptin signaling may be impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Agrp
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 13: PubMed34684588

2021

A Role for Peripheral Anandamide and 2-Arachidonoylglycerol in Short-Term Food Intake and Orexigenic Hypothalamic Responses in a Species with Continuous Nutrient Delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel van Ackern,Angela Kuhla,Björn Kuhla

Domestic animal endocrinology 65:71-79 PubMed30007131

2018

Influences of maternal nutrient restriction and arginine supplementation on visceral metabolism and hypothalamic circuitry of offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Ligia D Prezotto,Jennifer F Thorson,Pawel P Borowicz,Jena L Peine,Michelle Bedenbaugh,Stanley M Hileman,Clay A Lents,Joel S Caton,Kendall C Swanson
View all publications
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