Guinea pig Polyclonal Agrp antibody. Suitable for IHC-Fr and reacts with Rat, Sheep samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Agrp aa 50 to C-terminus.
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- Nutrients 13:2021A Role for Peripheral Anandamide and 2-Arachidonoylglycerol in Short-Term Food Intake and Orexigenic Hypothalamic Responses in a Species with Continuous Nutrient Delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel van Ackern,Angela Kuhla,Björn KuhlaPubMed 34684588
- Domestic animal endocrinology 65:71-792018Influences of maternal nutrient restriction and arginine supplementation on visceral metabolism and hypothalamic circuitry of offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLigia D Prezotto et. al.PubMed 30007131
Key facts
- Host species
- Guinea pig
- Storage buffer
pH: 7 - 7.4
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Agrp aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56473
Reactivity data
Select an application| IHC-Fr | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
| Cow | Predicted |
| Pig | Predicted |
| Sheep | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000.00000 - 1/2000.00000 | Notes Permeabilization suggested: 0.1% Triton X-100 in blocking buffer. |
Species Sheep | Dilution info 1/1000.00000 - 1/2000.00000 | Notes Permeabilization suggested: 0.1% Triton X-100 in blocking buffer. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Human, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Alternative names
AGRP_HUMAN, AGRT, ART, ASIP2, Agouti Related Protein Homolog, Agouti related neuropeptide, Agouti-related protein
Recommended products
Guinea pig Polyclonal Agrp antibody. Suitable for IHC-Fr and reacts with Rat, Sheep samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Agrp aa 50 to C-terminus.
Key facts
- Host species
- Guinea pig
- Storage buffer
pH: 7 - 7.4
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Agrp aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P56473
- Purity
- Whole antiserum
- Reconstitution
- Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. It is recommended that a thawed sample is stored at 4ºC for no longer than 2 weeks. Allocation of appropriate anti-bacterial agent can increase shelf life by several weeks. Diluted serum should be prepared as required. Long term stability requires storage, preferably in small aliquots at -20ºC or lower. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AGRP also known as Agouti-related protein is a neuropeptide with a molecular weight of approximately 13.5 kDa. It is predominantly expressed in the arcuate nucleus of the hypothalamus in the brain and some regions of the adrenal gland. AGRP serves as an antagonist to melanocortin receptors particularly the melanocortin 3 and 4 receptors (MC3R and MC4R) which are involved in energy homeostasis. By binding to these receptors AGRP plays a critical role in the regulation of appetite and energy balance by inhibiting the action of alpha-melanocyte-stimulating hormone.
- Biological function summary
AGRP is involved in the regulation of feeding behavior and body weight. It does not function as part of a larger complex but operates effectively as an antagonist in its own right. When expressed AGRP influences energy expenditure and food intake contributing to how the body regulates hunger and satiety. Its activity helps to modulate signaling pathways that can increase the desire to eat when energy stores are depleted.
- Pathways
AGRP plays a notable role in the melanocortin pathway and influences the hypothalamic signaling pathways related to energy balance. It interacts with melanocortin receptors as part of this system modulating signals that maintain energy homeostasis. AGRP is closely related to orexigenic pathways working alongside other proteins like neuropeptide Y (NPY) which further stimulate appetite and food intake.
- Associated diseases and disorders
AGRP has a strong connection to obesity and metabolic disorders. It is implicated in conditions where energy balance is disrupted leading to excessive weight gain. AGRP levels can be elevated in individuals with obesity suggesting a link between its upregulation and increased appetite. Additionally the protein interacts with leptin a hormone involved in energy expenditure and appetite suppression highlighting AGRP's role in metabolic conditions where leptin signaling may be impaired.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Frozen sections) - Anti-AGRP antibody (ab228495)
4% paraformaldehyde-fixed, frozen section of sheep hypothalamus tissue stained for AGRP using ab228495 at 1/1000 dilution in immunohistochemical analysis, followed by detection with a biotinylated secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-AGRP antibody (ab228495)
4% paraformaldehyde-fixed, frozen section of rat brain tissue stained for AGRP using ab228495 at 1/1000 dilution in immunohistochemical analysis, followed by detection with Alexa Fluor® 568 Goat anti-Guinea pig at 1/500 dilution.
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