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AB254558

Anti-AGRP antibody [EPR18155-110]

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(3 Publications )

Anti-AGRP antibody [EPR18155-110] (ab254558) is a rabbit monoclonal antibody detecting AGRP in IHC-P, IHC-Fr. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGRP antibody [EPR18155-110] (AB254558)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGRP antibody [EPR18155-110] (AB254558)

Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling AGRP with ab254558 at 1/100 dilution (5.58 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on paraventricular nucleus of mouse hypothalamus (PMID : 10433222; 9311920) is observed. The section was incubated with ab254558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemistry (Frozen sections) - Anti-AGRP antibody [EPR18155-110] (AB254558)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-AGRP antibody [EPR18155-110] (AB254558)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse hypothalamus tissue labeling ab254558 with AGRP at 1/500 (1.12 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Specific staining on arcuate hypothalamic nucleus (PMID : 23638108) is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGRP antibody [EPR18155-110] (AB254558)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGRP antibody [EPR18155-110] (AB254558)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling AGRP with ab254558 at 1/100 dilution (5.58 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on medullary cells of mouse adrenal gland (PMID : 28531318; 9311920) is observed. The section was incubated with ab254558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

  • Carrier free

    Anti-AGRP antibody [EPR18155-110] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18155-110

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>AGRP is synthesized in neuropeptide Y (NPY)-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. Please fix the tissue by perfusing the hypothalamus with freshly prepared 4% PFA and perform heat-mediated antigen retrieval using a sodium citrate buffer.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-AGRP antibody [EPR18155-110] (ab254558) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples.

Other related products
We have a range of other formats of antibody clone [EPR18155-110] also available for your convenience: ab254558, Carrier free - ab255686

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGRP also known as Agouti-related protein is a neuropeptide with a molecular weight of approximately 13.5 kDa. It is predominantly expressed in the arcuate nucleus of the hypothalamus in the brain and some regions of the adrenal gland. AGRP serves as an antagonist to melanocortin receptors particularly the melanocortin 3 and 4 receptors (MC3R and MC4R) which are involved in energy homeostasis. By binding to these receptors AGRP plays a critical role in the regulation of appetite and energy balance by inhibiting the action of alpha-melanocyte-stimulating hormone.
Biological function summary

AGRP is involved in the regulation of feeding behavior and body weight. It does not function as part of a larger complex but operates effectively as an antagonist in its own right. When expressed AGRP influences energy expenditure and food intake contributing to how the body regulates hunger and satiety. Its activity helps to modulate signaling pathways that can increase the desire to eat when energy stores are depleted.

Pathways

AGRP plays a notable role in the melanocortin pathway and influences the hypothalamic signaling pathways related to energy balance. It interacts with melanocortin receptors as part of this system modulating signals that maintain energy homeostasis. AGRP is closely related to orexigenic pathways working alongside other proteins like neuropeptide Y (NPY) which further stimulate appetite and food intake.

AGRP has a strong connection to obesity and metabolic disorders. It is implicated in conditions where energy balance is disrupted leading to excessive weight gain. AGRP levels can be elevated in individuals with obesity suggesting a link between its upregulation and increased appetite. Additionally the protein interacts with leptin a hormone involved in energy expenditure and appetite suppression highlighting AGRP's role in metabolic conditions where leptin signaling may be impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Signaling protein that functions as an antagonist of melanocyte-stimulating-hormone receptors MC3R and MC4R by precluding agonist-induced signaling, thereby inhibiting cAMP production within the hypothalamus and adrenal gland (PubMed : 9892020). Involved in the control of feeding behavior via the central melanocortin system (By similarity). Has very low activity towards MC5R (PubMed : 9892020). Also promotes endocytosis of MC3R and MC4R in an arrestin-dependent mechanism (By similarity).
See full target information Agrp

Alternative Names

Agrt, Art, Agrp, Agouti-related protein
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Publications (3)

Recent publications for all applications. Explore the full listand refine your search

Journal of lipid research 66:100736 PubMed39724960

2024

Microglial activation and hypothalamic structural plasticity in HFD obesity: insights from semaglutide and minocycline.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Rong,Fang Wei,Yuqi Jiang,Qintao Ma,Dongmei Wang,Jie Shen

Theranostics 14:6605-6624 PubMed39479445

2024

Hypothalamic P62 (SQSTM1) regulates energy balance by modulating leptin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Yang,Yang Li,Mingyuan Tian,Wuquan Deng,Dongfang Liu,Chen Chen,Zhiming Zhu,Hongting Zheng,Gangyi Yang,Ling Li,Mengliu Yang

Frontiers in oncology 11:650054 PubMed34277403

2021

Enzalutamide-Induced Upregulation of PCAT6 Promotes Prostate Cancer Neuroendocrine Differentiation by Regulating miR-326/HNRNPA2B1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Liu,Hui-Yang Jiang,Tao Yuan,Jie Luo,Wei-Dong Zhou,Qi-Quan Jiang,Denglong Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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