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AB283845

Anti-AGRP antibody [EPR22984-34] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal AGRP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

AGRT, ART, AGRP, Agouti-related protein

1 Images
Sandwich ELISA - Anti-AGRP antibody [EPR22984-34] - BSA and Azide free (Detector) (AB283845)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-AGRP antibody [EPR22984-34] - BSA and Azide free (Detector) (AB283845)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab282295).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22984-34

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab283845 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human AGRP. The recommended pair for sandwich ELISA is:
Capture: ab283844, Human AGRP Capture Antibody (unconjugated)
Detector: ab283845, Human AGRP Detector Antibody (unconjugated)
The reference range value is 15.63 - 1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AGRP also known as Agouti-related protein is a neuropeptide with a molecular weight of approximately 13.5 kDa. It is predominantly expressed in the arcuate nucleus of the hypothalamus in the brain and some regions of the adrenal gland. AGRP serves as an antagonist to melanocortin receptors particularly the melanocortin 3 and 4 receptors (MC3R and MC4R) which are involved in energy homeostasis. By binding to these receptors AGRP plays a critical role in the regulation of appetite and energy balance by inhibiting the action of alpha-melanocyte-stimulating hormone.
Biological function summary

AGRP is involved in the regulation of feeding behavior and body weight. It does not function as part of a larger complex but operates effectively as an antagonist in its own right. When expressed AGRP influences energy expenditure and food intake contributing to how the body regulates hunger and satiety. Its activity helps to modulate signaling pathways that can increase the desire to eat when energy stores are depleted.

Pathways

AGRP plays a notable role in the melanocortin pathway and influences the hypothalamic signaling pathways related to energy balance. It interacts with melanocortin receptors as part of this system modulating signals that maintain energy homeostasis. AGRP is closely related to orexigenic pathways working alongside other proteins like neuropeptide Y (NPY) which further stimulate appetite and food intake.

AGRP has a strong connection to obesity and metabolic disorders. It is implicated in conditions where energy balance is disrupted leading to excessive weight gain. AGRP levels can be elevated in individuals with obesity suggesting a link between its upregulation and increased appetite. Additionally the protein interacts with leptin a hormone involved in energy expenditure and appetite suppression highlighting AGRP's role in metabolic conditions where leptin signaling may be impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner.
See full target information AGRP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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