Anti-AGTR1 antibody [HL2526] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

ab325838 detects AGTR1 protein at cell membrane by immunofluorescent analysis. Sample : Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : AGTR1 stained by ab325838 diluted at 1 : 500. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

ab325838 detects AGTR1 protein by immunofluorescent analysis. Sample : Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : AGTR1 stained by ab325838 diluted at 1 : 500 and competitor's antibody diluted at 1 : 500. Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1 : 1000. Blue : Fluoroshield with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

ab325838 detects AGTR1 protein by immunohistochemical analysis. Sample : Paraffin-embedded human Huh-7 xenograft. AGTR1 stained by ab325838 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

ab325838 detects AGTR1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse kidney. AGTR1 stained by ab325838 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

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Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325838 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (ab325838) at 1/1000 dilution

All lanes:

PC-3 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

Huh-7 cytoplasma extracts and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325838 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (CE : cytoplasma extract; ME : membrane extract)

All lanes:

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (ab325838) at 1/500 dilution

Lane 1:

Huh-7 cytoplasma extracts at 30 µg

Lane 2:

Huh-7 membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325838 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (ab325838) at 1/1000 dilution

Lane 1:

HCT116 whole cell extracts at 30 µg

Lane 2:

A549 whole cell extracts at 30 µg

Lane 3:

MCF-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (AB325838)

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325838 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-AGTR1 antibody [HL2526] - BSA and Azide free (ab325838) at 1/1000 dilution

All lanes:

CT26 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true