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AB178699

Anti-AGXT antibody [EPR13232-59]

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(2 Publications)

Rabbit Recombinant Monoclonal AGXT antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

AGT1, SPAT, AGXT, Alanine--glyoxylate aminotransferase, AGT, Serine--pyruvate aminotransferase, SPT

1 Images
Western blot - Anti-AGXT antibody [EPR13232-59] (AB178699)
  • WB

Supplier Data

Western blot - Anti-AGXT antibody [EPR13232-59] (AB178699)

All lanes:

Western blot - Anti-AGXT antibody [EPR13232-59] (ab178699) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Predicted band size: 43 kDa

false

  • Carrier free

    Anti-AGXT antibody [EPR13232-59] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13232-59

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AGXT also known as alanine-glyoxylate aminotransferase plays an important role in human metabolism. It is a liver-specific enzyme with an approximate molecular weight of 43 kDa. AGXT is expressed primarily in liver peroxisomes where it catalyzes the transamination reaction that converts glyoxylate to glycine. This enzymatic activity is important for detoxifying glyoxylate which otherwise could accumulate and result in harmful effects. The expression level and activity of AGXT can be influenced by various metabolic conditions.
Biological function summary

The conversion of glyoxylate to glycine mediated by AGXT helps in maintaining cellular homeostasis. AGXT is not part of large protein complexes but it directly influences the pathways leading to amino acid metabolism. By converting glyoxylate into glycine this enzyme plays a part in managing the precursors necessary for the biosynthesis of other important biomolecules including serine and pyruvate. As glyoxylate is an important intermediary its conversion is essential for metabolic flux.

Pathways

AGXT has a significant role in the glyoxylate metabolic pathway and the glycine serine and threonine metabolism pathway. In these pathways it works closely with other enzymes like glycine decarboxylase and serine hydroxymethyltransferase. The proper functioning of these pathways ensures efficient energy utilization and detoxification in the human body. Any disruption can affect the balance of amino acid concentrations leading to metabolic disturbances.

AGXT is most prominently linked to primary hyperoxaluria type 1 (PH1) which is a rare genetic condition. In PH1 mutations in the AGXT gene lead to its dysfunctional enzyme causing the accumulation of oxalate and subsequent kidney stone formation and renal failure. The disease connection places AGXT in relation to key proteins involved in oxalate metabolism and renal function such as glycolate oxidase. Understanding AGXT's role in these disorders opens pathways for therapeutic interventions and advanced research into metabolic regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification (PubMed : 10960483, PubMed : 12777626, PubMed : 23229545, PubMed : 24055001, PubMed : 26149463). Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism (PubMed : 10347152).
See full target information AGXT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The CRISPR journal 5:422-434 PubMed35686982

2022

Efficient and Fast Generation of Relevant Disease Mouse Models by and Gene Editing of Zygotes.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Sanchez-Baltasar,Aida Garcia-Torralba,Virginia Nieto-Romero,Angustias Page,Andrea Molinos-Vicente,Sergio López-Manzaneda,Isabel Ojeda-Pérez,Angel Ramirez,Manuel Navarro,José Carlos Segovia,María García-Bravo

Cell transplantation 25:2221-2243 PubMed27512979

2016

Human Urinary Epithelial Cells as a Source of Engraftable Hepatocyte-Like Cells Using Stem Cell Technology.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Sauer,Tatyana Tchaikovskaya,Xia Wang,Yanfeng Li,Wei Zhang,Krisztina Tar,Zsuzsanna Polgar,Jianqiang Ding,Chandan Guha,Ira J Fox,Namita Roy-Chowdhury,Jayanta Roy-Chowdhury
View all publications
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Product promise

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