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AB178708

Anti-AGXT antibody [EPR13232]

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(4 Publications)

Rabbit Recombinant Monoclonal AGXT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications.

View Alternative Names

AGT1, SPAT, AGXT, Alanine--glyoxylate aminotransferase, AGT, Serine--pyruvate aminotransferase, SPT

4 Images
Immunoprecipitation - Anti-AGXT antibody [EPR13232] (AB178708)
  • IP

Supplier Data

Immunoprecipitation - Anti-AGXT antibody [EPR13232] (AB178708)

Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-AGXT antibody [EPR13232] (ab178708)

Predicted band size: 43 kDa

false

Western blot - Anti-AGXT antibody [EPR13232] (AB178708)
  • WB

Supplier Data

Western blot - Anti-AGXT antibody [EPR13232] (AB178708)

All lanes:

Western blot - Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 43 kDa

false

Western blot - Anti-AGXT antibody [EPR13232] (AB178708)
  • WB

Lab

Western blot - Anti-AGXT antibody [EPR13232] (AB178708)

Western blot analysis of Mouse liver (20μg) labelling AGXT with ab178708 at 1/2000 in 5% NFDM /TBST. A Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L), (ab97051) (1/20000) was used as the secondary antibody.

All lanes:

Western blot - Anti-AGXT antibody [EPR13232] (ab178708)

Predicted band size: 43 kDa

Observed band size: 40 kDa

false

Exposure time: 3min

Western blot - Anti-AGXT antibody [EPR13232] (AB178708)
  • WB

Lab

Western blot - Anti-AGXT antibody [EPR13232] (AB178708)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 70 kDa is unknown. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Agxt-KO homozygous mice (Strain ID : T030354).

All lanes:

Western blot - Anti-AGXT antibody [EPR13232] (ab178708) at 1/1000 dilution

Lane 1:

Wild-type mouse liver tissue lysate (male) at 20 µg

Lane 2:

Wild-type mouse liver tissue lysate (female) at 20 µg

Lane 3:

Agxt knockout mouse liver tissue lysate (male) at 20 µg

Lane 4:

Agxt knockout mouse liver tissue lysate (female) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 43 kDa,37 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-AGXT antibody [EPR13232] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13232

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target AGXT also known as alanine-glyoxylate aminotransferase plays an important role in human metabolism. It is a liver-specific enzyme with an approximate molecular weight of 43 kDa. AGXT is expressed primarily in liver peroxisomes where it catalyzes the transamination reaction that converts glyoxylate to glycine. This enzymatic activity is important for detoxifying glyoxylate which otherwise could accumulate and result in harmful effects. The expression level and activity of AGXT can be influenced by various metabolic conditions.
Biological function summary

The conversion of glyoxylate to glycine mediated by AGXT helps in maintaining cellular homeostasis. AGXT is not part of large protein complexes but it directly influences the pathways leading to amino acid metabolism. By converting glyoxylate into glycine this enzyme plays a part in managing the precursors necessary for the biosynthesis of other important biomolecules including serine and pyruvate. As glyoxylate is an important intermediary its conversion is essential for metabolic flux.

Pathways

AGXT has a significant role in the glyoxylate metabolic pathway and the glycine serine and threonine metabolism pathway. In these pathways it works closely with other enzymes like glycine decarboxylase and serine hydroxymethyltransferase. The proper functioning of these pathways ensures efficient energy utilization and detoxification in the human body. Any disruption can affect the balance of amino acid concentrations leading to metabolic disturbances.

AGXT is most prominently linked to primary hyperoxaluria type 1 (PH1) which is a rare genetic condition. In PH1 mutations in the AGXT gene lead to its dysfunctional enzyme causing the accumulation of oxalate and subsequent kidney stone formation and renal failure. The disease connection places AGXT in relation to key proteins involved in oxalate metabolism and renal function such as glycolate oxidase. Understanding AGXT's role in these disorders opens pathways for therapeutic interventions and advanced research into metabolic regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification (PubMed : 10960483, PubMed : 12777626, PubMed : 23229545, PubMed : 24055001, PubMed : 26149463). Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism (PubMed : 10347152).
See full target information AGXT
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26035 PubMed40676134

2025

Shift in the urinary metabolome associated with 2,3,7,8-tetrachlorodibenzo-p-dioxin activation of the hepatic aryl hydrocarbon receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Warren J Sink,Russell Fling,Ali Yilmaz,Rance Nault,Delanie Goniwiecha,Jack R Harkema,Stewart F Graham,Tim Zacharewski

Cell death discovery 11:106 PubMed40091075

2025

Investigating synthetic lethality and PARP inhibitor resistance in pancreatic cancer through enantiomer differential activity.

Applications

Unspecified application

Species

Unspecified reactive species

Mirco Masi,Laura Poppi,Viola Previtali,Shannon R Nelson,Kieran Wynne,Giulia Varignani,Federico Falchi,Marina Veronesi,Ennio Albanesi,Daniele Tedesco,Francesca De Franco,Andrea Ciamarone,Samuel H Myers,Jose Antonio Ortega,Greta Bagnolini,Giovanni Ferrandi,Fulvia Farabegoli,Nicola Tirelli,Giuseppina Di Stefano,Giorgio Oliviero,Naomi Walsh,Marinella Roberti,Stefania Girotto,Andrea Cavalli

iScience 27:109530 PubMed38577102

2024

Restored glyoxylate metabolism after AGXT gene correction and direct reprogramming of primary hyperoxaluria type 1 fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Virginia Nieto-Romero,Aida García-Torralba,Andrea Molinos-Vicente,Francisco José Moya,Sandra Rodríguez-Perales,Ramón García-Escudero,Eduardo Salido,José-Carlos Segovia,María García-Bravo

Nature communications 11:6127 PubMed33257663

2020

Lkb1 suppresses amino acid-driven gluconeogenesis in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre-Alexandre Just,Sara Charawi,Raphaël G P Denis,Mathilde Savall,Massiré Traore,Marc Foretz,Sultan Bastu,Salimata Magassa,Nadia Senni,Pierre Sohier,Maud Wursmer,Mireille Vasseur-Cognet,Alain Schmitt,Morgane Le Gall,Marjorie Leduc,François Guillonneau,Jean-Pascal De Bandt,Patrick Mayeux,Béatrice Romagnolo,Serge Luquet,Pascale Bossard,Christine Perret
View all publications
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Product promise

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