Rabbit Recombinant Monoclonal AGXT antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--western-blot-img148984.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74600.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079)")
![Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--immunoprecipitation-img74162.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109226.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079)")
![Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--western-blot-img158684.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (AB250079)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Expected |
| Mouse | Expected | Expected | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
Serine--pyruvate aminotransferase, SPT, Alanine--glyoxylate aminotransferase, AGT, AGXT, SPAT, AGT1
Recommended products
Rabbit Recombinant Monoclonal AGXT antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR13232
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab250079 is the carrier-free version of Anti-AGXT antibody [EPR13232] ab178708.
Mouse species is recommended by WB application. We have preliminary internal testing data to indicate this antibody may not react with mouse in IHC.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The biological target AGXT also known as alanine-glyoxylate aminotransferase plays an important role in human metabolism. It is a liver-specific enzyme with an approximate molecular weight of 43 kDa. AGXT is expressed primarily in liver peroxisomes where it catalyzes the transamination reaction that converts glyoxylate to glycine. This enzymatic activity is important for detoxifying glyoxylate which otherwise could accumulate and result in harmful effects. The expression level and activity of AGXT can be influenced by various metabolic conditions.
- Biological function summary
The conversion of glyoxylate to glycine mediated by AGXT helps in maintaining cellular homeostasis. AGXT is not part of large protein complexes but it directly influences the pathways leading to amino acid metabolism. By converting glyoxylate into glycine this enzyme plays a part in managing the precursors necessary for the biosynthesis of other important biomolecules including serine and pyruvate. As glyoxylate is an important intermediary its conversion is essential for metabolic flux.
- Pathways
AGXT has a significant role in the glyoxylate metabolic pathway and the glycine serine and threonine metabolism pathway. In these pathways it works closely with other enzymes like glycine decarboxylase and serine hydroxymethyltransferase. The proper functioning of these pathways ensures efficient energy utilization and detoxification in the human body. Any disruption can affect the balance of amino acid concentrations leading to metabolic disturbances.
- Associated diseases and disorders
AGXT is most prominently linked to primary hyperoxaluria type 1 (PH1) which is a rare genetic condition. In PH1 mutations in the AGXT gene lead to its dysfunctional enzyme causing the accumulation of oxalate and subsequent kidney stone formation and renal failure. The disease connection places AGXT in relation to key proteins involved in oxalate metabolism and renal function such as glycolate oxidase. Understanding AGXT's role in these disorders opens pathways for therapeutic interventions and advanced research into metabolic regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--western-blot-img148984.jpg "Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)")
Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
This data was developed using Anti-AGXT antibody [EPR13232] ab178708, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-AGXT antibody [EPR13232] (Anti-AGXT antibody [EPR13232] ab178708) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: Human fetal liver tissue lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74600.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
This data was developed using Anti-AGXT antibody [EPR13232] ab178708, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AGXT with Anti-AGXT antibody [EPR13232] ab178708 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--immunoprecipitation-img74162.jpg "Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)")
Immunoprecipitation - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
This data was developed using Anti-AGXT antibody [EPR13232] ab178708, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (1) Human fetal liver cell lysate or (2) 1X PBS (negative control) immunoprecipitated using Anti-PACAP antibody [EPR11543(B)] ab170891 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-AGXT antibody [EPR13232] (Anti-AGXT antibody [EPR13232] ab178708)
Predicted band size: 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109226.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
This data was developed using Anti-AGXT antibody [EPR13232] ab178708, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with Anti-AGXT antibody [EPR13232] ab178708 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr13232-bsa-and-azide-free-ab250079--western-blot-img158684.png "Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)")
Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
This data was developed using Anti-AGXT antibody [EPR13232] ab178708, the same antibody clone in a different buffer formulation.
Western blot analysis of Mouse liver (20μg) labelling AGXT with Anti-AGXT antibody [EPR13232] ab178708 at 1/2000 in 5% NFDM/TBST. A Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L), (Goat Anti-Rabbit IgG H&L (HRP) ab97051) (1/20000) was used as the secondary antibody.
All lanes: Western blot - Anti-AGXT antibody [EPR13232] - BSA and Azide free (ab250079)
Predicted band size: 43 kDa
Observed band size: 40 kDa
Exposure time: 3min
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