Rabbit Recombinant Monoclonal AGXT antibody. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Images
![Western blot - Anti-AGXT antibody [EPR22885-58] (AB261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--western-blot-img12099.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-AGXT antibody [EPR22885-58] (AB261910)")
![Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] (AB261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--flow-cytometry-intracellular-img37256.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] (AB261910)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (AB261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110612.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (AB261910)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (AB261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26677.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (AB261910)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (AB261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128040.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (AB261910)")
Publications
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- Biomolecules 12:2022Alanine-Glyoxylate Aminotransferase Sustains Cancer Stemness Properties through the Upregulation of SOX2 and OCT4 in Hepatocellular Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Ye,Xiaoxia Chi,Xiuwen Yan,Fangqin Wu,Zhigang Liang,Wen-Hao YangPubMed 35625596
- Cell reports 36:1095262021Epigenomic and transcriptional profiling identifies impaired glyoxylate detoxification in NAFLD as a risk factor for hyperoxaluria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKathrin Gianmoena,Nina Gasparoni,Adelina Jashari,Philipp Gabrys,Katharina Grgas,Ahmed Ghallab,Karl Nordström,Gilles Gasparoni,Jörg Reinders,Karolina Edlund,Patricio Godoy,Alexander Schriewer,Heiko Hayen,Christian A Hudert,Georg Damm,Daniel Seehofer,Thomas S Weiss,Peter Boor,Hans-Joachim Anders,Manga Motrapu,Peter Jansen,Tobias S Schiergens,Maren Falk-Paulsen,Philip Rosenstiel,Clivia Lisowski,Eduardo Salido,Rosemarie Marchan,Jörn Walter,Jan G Hengstler,Cristina CadenasPubMed 34433051
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Expected | Expected | Tested |
| Mouse | Not recommended | Tested | Not recommended | Tested | Tested | Tested |
| Rat | Not recommended | Tested | Not recommended | Expected | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). This antibody is not recommended for rat IHC-fr. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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5 products for Alternative Product
Target data
Function
Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification (PubMed:10960483, PubMed:12777626, PubMed:23229545, PubMed:24055001, PubMed:26149463). Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism (PubMed:10347152).
Alternative names
AGT1, SPAT, AGXT, Alanine--glyoxylate aminotransferase, AGT, Serine--pyruvate aminotransferase, SPT
Recommended products
Rabbit Recombinant Monoclonal AGXT antibody. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22885-58
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not recommended for rat IHC-fr.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The biological target AGXT also known as alanine-glyoxylate aminotransferase plays an important role in human metabolism. It is a liver-specific enzyme with an approximate molecular weight of 43 kDa. AGXT is expressed primarily in liver peroxisomes where it catalyzes the transamination reaction that converts glyoxylate to glycine. This enzymatic activity is important for detoxifying glyoxylate which otherwise could accumulate and result in harmful effects. The expression level and activity of AGXT can be influenced by various metabolic conditions.
- Biological function summary
The conversion of glyoxylate to glycine mediated by AGXT helps in maintaining cellular homeostasis. AGXT is not part of large protein complexes but it directly influences the pathways leading to amino acid metabolism. By converting glyoxylate into glycine this enzyme plays a part in managing the precursors necessary for the biosynthesis of other important biomolecules including serine and pyruvate. As glyoxylate is an important intermediary its conversion is essential for metabolic flux.
- Pathways
AGXT has a significant role in the glyoxylate metabolic pathway and the glycine serine and threonine metabolism pathway. In these pathways it works closely with other enzymes like glycine decarboxylase and serine hydroxymethyltransferase. The proper functioning of these pathways ensures efficient energy utilization and detoxification in the human body. Any disruption can affect the balance of amino acid concentrations leading to metabolic disturbances.
- Associated diseases and disorders
AGXT is most prominently linked to primary hyperoxaluria type 1 (PH1) which is a rare genetic condition. In PH1 mutations in the AGXT gene lead to its dysfunctional enzyme causing the accumulation of oxalate and subsequent kidney stone formation and renal failure. The disease connection places AGXT in relation to key proteins involved in oxalate metabolism and renal function such as glycolate oxidase. Understanding AGXT's role in these disorders opens pathways for therapeutic interventions and advanced research into metabolic regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--western-blot-img12099.jpg "Western blot - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Western blot - Anti-AGXT antibody [EPR22885-58] (ab261910)
Blocking and diluting buffer and concentration: 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID: 16309382).
Exposure time: Lanes 1/2: 1 second; Lane 3: 59 seconds.
All lanes: Western blot - Anti-AGXT antibody [EPR22885-58] (ab261910) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Rat liver tissue lysate at 20 µg
SecondaryLane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/250 dilution
Lanes 2 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
![Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--flow-cytometry-intracellular-img37256.jpg "Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] (ab261910)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling AGXT with ab261910 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110612.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AGXT with ab261910 at 1/1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in rat liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26677.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in mouse liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128040.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human liver cancer (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human liver (PMID: 29883780) is observed. The section was incubated with ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
![Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-frozen-sections-img71449.jpg "Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse liver tissue labeling AGXT with ab261910 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse liver (PMID: 29883780).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
![Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-ab261910--immunohistochemistry-frozen-sections-img71449.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] (ab261910)")
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