Rabbit Recombinant Monoclonal AGXT antibody. Carrier free. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--flow-cytometry-intracellular-img37256.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110612.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26677.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128040.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (AB261914)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-Fr | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Expected | Expected | Tested |
| Mouse | Not recommended | Tested | Not recommended | Tested | Tested | Tested |
| Rat | Not recommended | Tested | Not recommended | Expected | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). This antibody is not recommended for rat IHC-fr. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Peroxisomal aminotransferase that catalyzes the transamination of glyoxylate to glycine and contributes to the glyoxylate detoxification (PubMed:10960483, PubMed:12777626, PubMed:23229545, PubMed:24055001, PubMed:26149463). Also catalyzes the transamination between L-serine and pyruvate and contributes to gluconeogenesis from the L-serine metabolism (PubMed:10347152).
Alternative names
AGT1, SPAT, AGXT, Alanine--glyoxylate aminotransferase, AGT, Serine--pyruvate aminotransferase, SPT
Recommended products
Rabbit Recombinant Monoclonal AGXT antibody. Carrier free. Suitable for WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22885-58
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not recommended for rat IHC-fr.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab261914 is the carrier-free version of Anti-AGXT antibody [EPR22885-58] ab261910.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The biological target AGXT also known as alanine-glyoxylate aminotransferase plays an important role in human metabolism. It is a liver-specific enzyme with an approximate molecular weight of 43 kDa. AGXT is expressed primarily in liver peroxisomes where it catalyzes the transamination reaction that converts glyoxylate to glycine. This enzymatic activity is important for detoxifying glyoxylate which otherwise could accumulate and result in harmful effects. The expression level and activity of AGXT can be influenced by various metabolic conditions.
- Biological function summary
The conversion of glyoxylate to glycine mediated by AGXT helps in maintaining cellular homeostasis. AGXT is not part of large protein complexes but it directly influences the pathways leading to amino acid metabolism. By converting glyoxylate into glycine this enzyme plays a part in managing the precursors necessary for the biosynthesis of other important biomolecules including serine and pyruvate. As glyoxylate is an important intermediary its conversion is essential for metabolic flux.
- Pathways
AGXT has a significant role in the glyoxylate metabolic pathway and the glycine serine and threonine metabolism pathway. In these pathways it works closely with other enzymes like glycine decarboxylase and serine hydroxymethyltransferase. The proper functioning of these pathways ensures efficient energy utilization and detoxification in the human body. Any disruption can affect the balance of amino acid concentrations leading to metabolic disturbances.
- Associated diseases and disorders
AGXT is most prominently linked to primary hyperoxaluria type 1 (PH1) which is a rare genetic condition. In PH1 mutations in the AGXT gene lead to its dysfunctional enzyme causing the accumulation of oxalate and subsequent kidney stone formation and renal failure. The disease connection places AGXT in relation to key proteins involved in oxalate metabolism and renal function such as glycolate oxidase. Understanding AGXT's role in these disorders opens pathways for therapeutic interventions and advanced research into metabolic regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--flow-cytometry-intracellular-img37256.jpg "Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
Flow Cytometry (Intracellular) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling AGXT with Anti-AGXT antibody [EPR22885-58] ab261910 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGXT antibody [EPR22885-58] ab261910).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110612.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AGXT with Anti-AGXT antibody [EPR22885-58] ab261910 at 1/1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in rat liver (PMID: 29883780) is observed. The section was incubated with Anti-AGXT antibody [EPR22885-58] ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGXT antibody [EPR22885-58] ab261910).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26677.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling AGXT with Anti-AGXT antibody [EPR22885-58] ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in mouse liver (PMID: 29883780) is observed. The section was incubated with Anti-AGXT antibody [EPR22885-58] ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGXT antibody [EPR22885-58] ab261910).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128040.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling AGXT with Anti-AGXT antibody [EPR22885-58] ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human liver cancer (PMID: 29883780) is observed. The section was incubated with Anti-AGXT antibody [EPR22885-58] ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGXT antibody [EPR22885-58] ab261910).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling AGXT with Anti-AGXT antibody [EPR22885-58] ab261910 at 1/ 1000 dilution (0.502ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in human liver (PMID: 29883780) is observed. The section was incubated with Anti-AGXT antibody [EPR22885-58] ab261910 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGXT antibody [EPR22885-58] ab261910).
![Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-frozen-sections-img71449.jpg "Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen section of mouse liver tissue labeling AGXT with Anti-AGXT antibody [EPR22885-58] ab261910 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (green). The nuclear counter stain is DAPI (blue). Positive staining on mouse liver (PMID: 29883780).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-AGXT antibody [EPR22885-58] ab261910).
![Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914), expandable thumbnail](https://content.abcam.com/products/images/agxt-antibody-epr22885-58-bsa-and-azide-free-ab261914--immunohistochemistry-frozen-sections-img71449.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-AGXT antibody [EPR22885-58] - BSA and Azide free (ab261914)")
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