Rabbit Polyclonal AGXT2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Alanine--glyoxylate aminotransferase 2, mitochondrial aa 200 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Alanine--glyoxylate aminotransferase 2, mitochondrial aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q3UEG6
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.20000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-20.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Multifunctional aminotransferase with a broad substrate specifcity (PubMed:23023372, PubMed:23154179, PubMed:23280748, PubMed:27062388). Catalyzes the conversion of glyoxylate to glycine using alanine as the amino donor (By similarity). Catalyzes metabolism of not L- but the D-isomer of D-beta-aminoisobutyric acid to generate 2-methyl-3-oxopropanoate and alanine (PubMed:27062388). Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate (PubMed:27062388). Can metabolize NG-monomethyl-L-arginine (NMMA), asymmetric NG,NG-dimethyl-L-arginine (ADMA) and symmetric NG,N'G-dimethyl-L-arginine (SDMA) (PubMed:23023372, PubMed:23154179, PubMed:23280748). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure (PubMed:23023372).
Alternative names
AGT 2, (R)-3-amino-2-methylpropionate--pyruvate transaminase, Beta-ALAAT II, Beta-alanine-pyruvate aminotransferase, D-3-aminoisobutyrate-pyruvate aminotransferase, D-AIBAT, D-beta-aminoisobutyrate-pyruvate aminotransferase, Agxt2
Recommended products
Rabbit Polyclonal AGXT2 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Alanine--glyoxylate aminotransferase 2, mitochondrial aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Alanine--glyoxylate aminotransferase 2, mitochondrial aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q3UEG6
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab232803 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
AGXT2 also known as alanine--glyoxylate aminotransferase 2 is an enzyme with approximately 56 kDa mass. The enzyme is expressed mainly in the liver kidney and heart. AGXT2 catalyzes the transamination reaction between alanine and glyoxylate to produce pyruvate and glycine. It is critical for the cellular detoxification processes and the regulation of amino acid metabolism.
- Biological function summary
This enzyme contributes to the conversion of glyoxylate therefore preventing its accumulation which can be harmful. AGXT2 is not part of a larger protein complex but it works closely with other enzyme systems involved in amino acid metabolism. Its activity maintains the balance between glycolate and glycine levels within the body which is essential for normal cellular function.
- Pathways
AGXT2 functions in both the glyoxylate and dicarboxylate metabolism pathway as well as the broader amino acid metabolism pathways. Within these pathways its role connects it to other related proteins such as Glyoxylate reductase/hydroxypyruvate reductase (GRHPR) and Lactate dehydrogenase. These interactions contribute to maintaining homeostasis in intermediary metabolism.
- Associated diseases and disorders
AGXT2 abnormalities relate closely to primary hyperoxaluria type 2 and certain cardiovascular diseases. Mutations or deficiencies in AGXT2 can result in the overproduction of oxalate leading to kidney stone formation and renal damage. Additionally reduced AGXT2 activity might influence homocysteine levels potentially linking it to cardiovascular disorders. This relationship places AGXT2 in connection with other proteins such as cystathionine beta-synthase in the context of homocysteine metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-AGXT2 antibody (ab232803)
All lanes: Western blot - Anti-AGXT2 antibody (ab232803) at 2 µg/mL
All lanes: Mouse kidney lysate
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (ab232803)
Paraffin-embedded mouse liver tissue stained for AGXT2 using ab232803 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-AGXT2 antibody (ab232803)
All lanes: Western blot - Anti-AGXT2 antibody (ab232803) at 2 µg/mL
All lanes: Mouse liver lysate
Predicted band size: 57 kDa
Western blot - Anti-AGXT2 antibody (ab232803)
All lanes: Western blot - Anti-AGXT2 antibody (ab232803) at 2 µg/mL
All lanes: Recombinant mouse AGXT2 protein
Predicted band size: 57 kDa
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