Anti-AGXT2 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-AGXT2 antibody (AB237622)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AGXT2 using ab237622 at a dilution of 1/233 in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

Paraffin-embedded human kidney tissue stained for AGXT2 with ab237622 at a 1/700 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AGXT2 antibody (AB237622)

Paraffin-embedded human liver tissue stained for AGXT2 with ab237622 at a 1/700 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-AGXT2 antibody (AB237622)

All lanes:

Western blot - Anti-AGXT2 antibody (ab237622) at 1/500 dilution

All lanes:

LO2 (human immortal liver cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 57 kDa

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