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Rabbit Recombinant Monoclonal AHA1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (AB307716), expandable thumbnail
  • Western blot - Anti-AHA1 antibody [EPR26852-47] (AB307716), expandable thumbnail
  • Western blot - Anti-AHA1 antibody [EPR26852-47] (AB307716), expandable thumbnail
  • Western blot - Anti-AHA1 antibody [EPR26852-47] (AB307716), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity (PubMed:29127155). Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360). Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:29127155).

Alternative names

C14orf3, HSPC322, AHSA1, Activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, p38

Recommended products

Rabbit Recombinant Monoclonal AHA1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26852-47
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AHA1 also known as activator of heat shock 90kDa protein ATPase homolog 1 is a co-chaperone protein that plays a role in the regulation of HSP90 ATPase activity. This protein has a molecular weight of approximately 38 kDa. AHA1 expression occurs in various tissues with higher amounts seen in metabolically active tissues. It interacts directly with HSP90 enhancing the chaperone's ability to fold client proteins correctly by stimulating its ATPase activity.

Biological function summary

AHA1 modulates the function of the HSP90 chaperone complex. This complex is important for the stabilization and activation of many client proteins such as steroid hormone receptors and kinases. AHA1 binds to the middle domain of HSP90 contributing to conformational changes necessary for efficient ATP hydrolysis. By influencing these molecular processes AHA1 plays a role in maintaining cellular protein homeostasis under stress conditions.

Pathways

The HSP90-AHA1 interaction is significant in the signaling pathway of cellular stress responses and protein folding. AHA1's upregulating effect on HSP90 ATPase activity facilitates the correct folding and function of client proteins involved in the MAPK/ERK and PI3K/AKT pathways. These pathways commonly involve interactions with proteins such as RAF and PI3K which are important in cell proliferation and survival processes.

Associated diseases and disorders

AHA1 influences the progression of cancer and neurodegenerative diseases. Altered expression or function of AHA1 is linked to the development and progression of cancer as it affects the stability of oncoproteins through its interaction with HSP90. The protein is also connected to neurodegenerative diseases with disruptions in its function or expression impacting the folding and maintenance of neuronal proteins. In these diseases the AHA1-HSP90 interaction remains a critical component of pathogenic pathways affecting disease manifestation and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (ab307716), expandable thumbnail

    Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (ab307716)

    AHA1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg with ab307716 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307716 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg

    Lane 2: ab307716 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of 307716 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    All lanes: Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/30 dilution

    All lanes: HeLa whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 42 kDa

    Exposure time: 15s

  • Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716), expandable thumbnail

    Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 92 seconds

    All lanes: Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/1000 dilution

    Lane 1: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 6: Rat colon tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 42 kDa

    Exposure time: 92s

  • Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716), expandable thumbnail

    Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 70 seconds

    All lanes: Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human tonsil tissue lysate at 20 µg

    Lane 3: Human colon tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 42 kDa

    Exposure time: 70s

  • Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716), expandable thumbnail

    Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/1000 dilution

    Lane 1: 293T transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: 293T transfected with siRNA specifically targeting AHA1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 42 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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