Anti-AHA1 antibody [EPR26852-47]

• IP

Supplier Data

Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (AB307716)

AHA1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg with ab307716 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307716 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg Lane 2 : ab307716 IP in HeLa whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of 307716 in HeLa whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/30 dilution

All lanes:

HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 42 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-AHA1 antibody [EPR26852-47] (AB307716)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 70 seconds

All lanes:

Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 42 kDa

false

Exposure time: 70s

• WB

Lab

Western blot - Anti-AHA1 antibody [EPR26852-47] (AB307716)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/1000 dilution

Lane 1:

293T transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

293T transfected with siRNA specifically targeting AHA1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-AHA1 antibody [EPR26852-47] (AB307716)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 92 seconds

All lanes:

Western blot - Anti-AHA1 antibody [EPR26852-47] (ab307716) at 1/1000 dilution

Lane 1:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa

false

Exposure time: 92s