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AB249942

Anti-AHAS antibody [EPR12843] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AHAS antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AHAS, ILVBL, 2-hydroxyacyl-CoA lyase 2, Acetolactate synthase-like protein, IlvB-like protein

2 Images
Flow Cytometry (Intracellular) - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)

This data was developed using ab177157, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Hela cells labeling AHAS with ab177157 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

Western blot - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)
  • WB

Supplier Data

Western blot - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)

This data was developed using ab177157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AHAS antibody [EPR12843] (<a href='/en-us/products/primary-antibodies/ahas-antibody-epr12843-ab177157'>ab177157</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12843

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249942 is the carrier-free version of ab177157.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The acetohydroxyacid synthase (AHAS) also known as acetolactate synthase (ALS) is an enzyme that plays an important role in the biosynthesis of branched-chain amino acids. With a molecular mass of approximately 170 kDa AHAS catalyzes the first common step in the biosynthetic pathways of valine leucine and isoleucine. This enzyme is widely expressed in plants bacteria and fungi. AHAS is a holoenzyme that consists of a large catalytic subunit and a smaller regulatory subunit showing its complex structure and diverse regulatory control.
Biological function summary

AHAS facilitates the condensation of two pyruvate molecules to form acetolactate or a pyruvate and ketoisovalerate to form acetohydroxybutyrate. This condensation reaction is a critical step in the synthesis of all three branched-chain amino acids which are essential components for protein construction in various organisms. As a part of a multi-enzyme complex the activity of AHAS is also modulated by feedback mechanisms from the end products of its pathway ensuring the balance in amino acid production and cellular homeostasis.

Pathways

AHAS integrates into the branched-chain amino acid biosynthesis pathway directly taking its place at the start. This pathway connects to amino acid metabolism which is essential for protein synthesis. AHAS is intertwined with proteins like dihydroxyacid dehydratase and branched-chain aminotransferase which continue the pathway downstream. These interactions highlight AHAS's significance in creating amino acids necessary for organismal growth and development.

AHAS relates prominently to conditions involving imbalances in amino acid metabolism. Alterations in AHAS function can lead to metabolic disorders such as maple syrup urine disease although it primarily involves branched-chain alpha-keto acid dehydrogenase complex. In some cases herbicides target AHAS poisoning the mechanism in plants and disrupting their growth. Understanding the function of AHAS helps us to comprehend the architecture of metabolic diseases and assists in designing therapeutic approaches even though the direct connection with human metabolic disorders remains mainly indirect.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum 2-OH acyl-CoA lyase involved in the cleavage (C1 removal) reaction in the fatty acid alpha-oxydation in a thiamine pyrophosphate (TPP)-dependent manner. Involved in the phytosphingosine degradation pathway.
See full target information ILVBL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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