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AB223762

Anti-AHNAK antibody

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(1 Publication)

Rabbit Polyclonal AHNAK antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AHNAK aa 5550 to C-terminus.

View Alternative Names

PM227, AHNAK, Neuroblast differentiation-associated protein AHNAK, Desmoyokin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK antibody (AB223762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHNAK antibody (AB223762)

Paraffin-embedded human oral mucosa tissue stained for AHNAK using ab223762 at 1/20 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-AHNAK antibody (AB223762)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AHNAK antibody (AB223762)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for AHNAK (green) using ab223762 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AHNAK aa 5550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q09666

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The AHNAK protein also known as "giant ankyrin repeat-containing protein" or "desmoyokin" has a large molecular mass of approximately 700 kDa. It is expressed in various tissues including heart skeletal muscle and brain. The protein is characterized by its repeated ankyrin-like motifs which are essential for protein-protein interactions. AHNAK is localized to the plasma membrane cytoplasm and sometimes the nucleus indicating its versatile roles within cells.
Biological function summary

AHNAK has key functions in cell structure and signaling. It can form a complex with other proteins such as S100 family proteins influencing calcium binding and signal transduction. This complex plays a role in the regulation of the cytoskeleton and membrane repair processes. Additionally AHNAK participates in cellular stress responses and impacts cell adhesion by interacting with membrane-associated proteins.

Pathways

AHNAK interacts with pathways involving calcium signaling and cytoskeletal organization. It links to the calcium-binding protein S100B facilitating downstream signaling that impacts cellular responses to external stimuli. Another pathway involves plasma membrane repair where AHNAK partners with annexin proteins to reseal damaged membranes and maintain cellular integrity.

AHNAK associates with cardiomyopathies and certain types of cancer. In cardiac conditions its interaction with proteins like dystrophin is important for maintaining muscle cell integrity during contraction. The dysregulation of AHNAK in cancer can influence cell motility and invasiveness highlighting its potential as a biomarker for tumor progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be required for neuronal cell differentiation.
See full target information AHNAK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2179 PubMed35449133

2022

Golgi apparatus-targeted aggregation-induced emission luminogens for effective cancer photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Minglun Liu,Yuncong Chen,Yan Guo,Hao Yuan,Tongxiao Cui,Shankun Yao,Suxing Jin,Huanhuan Fan,Chengjun Wang,Ran Xie,Weijiang He,Zijian Guo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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